The Use of Enterococci as Probiotics in Poultry

Document Type: Review Article

Author

North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

Abstract

Enterococci are members of the lactic acid bacteria family and are responsible for many food spoilage and fermentations. Some strains of this microorganism are used as probiotics in humans and animals to improve host immunity. However, some Enterococci are important pathogens which cause severe infections. Some strains of Enterococci are resistant to common antibiotics. The Enterococcus faecium and Enterococcus faecalis strains are more common probiotics. Such probiotics are used as an alternative to growth promoting antibiotics, which their use has been restricted. In domestic animals, enterococcal probiotics are mostly used to cure or prevent pathogen infections and immune response and growth performance improvement. This review covers the reports on the application of Enterococcus genus as a functional probiotic in poultry. The results suggest that Enterococcus faecium is a safe probiotic and improve the immune system and performance of broiler chickens.

Keywords


INTRODUCTION

The intensive systems of broiler chicken production could be accompanied with some stressor factors (Panda et al. 2006). In the recent decades, the uncontrolled use of growth promoting antibiotics has been increasing the risk of developing of antibiotic resistant pathogens (Sorum and Sunde, 2001). In 1996, the scientific findings and public concerns, was resulted in ban of growth promoter antibiotic application in the European Union. The new situation, was triggered more intensive research to find new safe animal growth promoter alternatives, such as changing the gut microflora using live non-pathogenic microorganisms with promising effects on birds health and performance which are known as probiotics. Probiotics have shown promising effects as alternatives to growth-promoting (Awad et al. 2009). The positive effects of probiotics on immune responses (Capcarova et al. 2008; Lee et al. 2008), decreasing pathogenic flora in the intestine has been widely accepted (Crawford, 1979). The lactic acid producing bacteria are the main probiotics and in particular Lactobacillus acidophilus, L. casei, L. reuteri are the base of most lactic acid bacteria based products (Caglar et al. 2005). Poultry initiates to eat solid feed immediately after hatching, then probiotics consumption must start in the early ages when gut microflora are not still well developed (Vahjen et al. 2002). The probiotic bacteria must also have additional criteria, including resistance to gastrointestinal pH and bile salts which are prerequisites for survival, colonization and action of ingested bacteria in the intestinal tract of the host (Erkkila and Petaja, 2000; Liong and Shah, 2005). The other essential feature to screening bacteria as potential probiotic is sensitivity to antibiotics, because bacteria can contain viru lence factors and antibiotic resistance factors. The most abundant lactic acid bacteria in the intestine of chickens are Lactobacillus and Enterococcus (Mitsuoka, 2002). The present review tries to summarize the findings and reports on the Enterococcus genus a functional probiotic in poultry.

 

Enterococcus genus

Until 1980 s, whole the Gram-positive cocci were known as streptococci, however the microbiological progress was reported them to the new genera Enterococcus, Lactococcus and Streptococcus (Schleifer and Kilpper-Bälz, 1984; Devriese et al. 1993; Devriese and Pot, 1995). The genus Enterococcus is a member of the lactic acid bacteria family and significant bacteria in cheese production, and spoilage. The Enterococcus tolerates to high salt and pH then usually is dominant in fermented foods. More than 37 species have been recognized for the genus Enterococcus and this genus are found in the environment and many animal and human based materials (Devriese et al. 1991; Devriese et al. 2003; Franz and Holzapfel, 2006). The probiotic characteristics have been recognized for some enterococcal strains and they have effectively used in human and animals. However, there are also some photogenic enterococcal strains which are responsible for bacteraemia, endocarditis or urinary tract infections in human. The pathogen Enterococcus strains are usually antibiotic resistance and there are concerns for the secure application as probiotics (Franz et al. 2011). On the other hand, E. faecium, E. faecalis, E. hirae, E. durans, and E. cecorum are natural residents of the farm animal’s intestinal tract, which is an essential factor to probiotic survival (Devriese et al. 1991; Devriese et al. 1994; Leclercq et al. 1996). From a probiotic point of view, the facultative anaerobic bacteria, E. faecium and E. faecalis are the main enterococcal species and E. faecium is permitted by the Association of American Feed Control Officials, fed to broiler chickens as a probiotic supplement (Franz et al. 1999; Foulquié-Moreno et al. 2006; Zhao et al. 2013). The ability of chicken originated Enterococcus spp. (E. faecium EF55) to produce bacteriocins as potential antimicrobial factors could confirm their probiotic effects for poultry (Laukova et al. 2004).

 

Effect of enterococcal probiotics on intestinal microbial population

Vahjen et al. (2002) reported that dietary supplementation of E. faecium SF68 increased the lactic acid bacteria population in turkey small intestine. This confirms that the enterococci can tolerate turkey gastrointestinal tract condition and control pathogenic bacteria. Samli et al. (2007) found that dietary supplement of E. faecium could increase lactic acid bacteria colonization in the ileum, and increased their excretion (Samli et al. 2007). A multi species probiotic containing Enterococcus, Bifidobacterium and Pediococcus strains applied in the feed and water manipulated the cecal microbial population, such that increased the Lactobacilli, Bifidobacteria and gram-positive cocci and also reduced the Salmonella population in turkey and broilers (Mountzouris et al. 2007; Grimes et al. 2008; Capcarova et al. 2010). In the study of Samli et al. (2010), the E. faecium probiotic improved the ileal and cecal microbial population and significantly reduced the Escherichia coli population. Kralik et al. (2004) also demonstrated the effect of dietary supplementation of E. faecium on E. coli reduction in broilers. The positive effect of E. faecium on fecal microflora of broiler has also reported by Kacaniova et al. (2006). In another study, E. faecium CCM8558 effectively colonized in the intestinal tract of chickens and reduced the Campylobacter spp load (Laukova et al. 2017). Cao et al. (2013) fed E. faecium to broilers and showed lower E. coli and C. perfringens population and higher Lactobacillus and Bifidobacterium population in the cecum contents. Levkut et al. (2009) observed that the E. faecium EF55 decreased the cecal population of Salmonella in the infected broilers. Similarly, other researchers have reported that Lactobacillus acidophilus and E. faecium based probiotics, decrease the Campylobacter jejuni count in chicks (Willis and Reid, 2008; Ghareeb et al. 2012). Table 1 shows the effects of E. faecium based probiotics on intestinal microbial population.

 

Effect of enterococcal probiotics on poultry performance

Samli et al. (2007) reported that dietary supplementation of E. faecium NCIMB 10415 improved broiler chickens weight gain and feed efficiency. The same findings have been reported by Mountzouris et al. (2007) and Awad et al. (2009). In another study using a multi species containing Lactobacillus, Pediococcus, Bifidobacterium and Enterococcus strains in feed and water, Mountzouris et al. (2007) found a growth rate comparable to feeding 2.5 mg/kg avilamycin antibiotic. Surprisingly, probiotic included in drinking water was more effective than dietary route. Demeterová et al. (2009) studied the supplementation of E. faecium DSM 7134 and natural humic substances in broiler chickens. The improved feed conversion ratio in birds fed both the supplements together was attributed to the increased phagocytes (Demeterová et al. 2009). Capcarova et al. (2010) used E. faecium probiotic in the diet of broiler chickens and found a decrease in feed intake without any change in feed conversion ratio. Luo et al. (2013) reported a normal growth rate in broiler chickens fed E. faecium supplement, and Zheng et al. (2015) found that dietary E. faecium inclusion did not influence the weight gain and feed intake of broilers, however, the feed conversion ratio was improved.

 

Table 1 The effects of Enterococcus probiotic on poultry intestinal microbial population

 

Cao et al. (2013) found that E. faecium probiotic improved the growth rate of chickens experimentally infected with pathogenic E. coli K88. In the study of Majidi-Mosleh et al. (2017), in ovo injection of E. faecium had no effect on hatchability our growth performance in broiler chickens. Zheng et al. (2016) suggested that dietary E. faecium feeding may change the partitioning of nutrients, consequently, improve nutrient utilization. Table 2 shows the effects of E. faecium based probiotics on performance traits of broilers.

 

Effect of enterococcal probiotics on poultry meat quality

Zheng et al. (2015) used 2D-DIGE-based proteomics to study the proteome changes in the meat of broilers fed E. faecium probiotic. The E. faecium supplement increased pH, water holding capacity and meat colour of pectoral muscle, however reduced abdominal fat content. They suggested that meat quality alterations following E. faecium feeding were due to changes in expression of 22 proteins in the pectoral muscle, such that dietary E. faecium probiotic improved meat quality of broilers. This was due to the changes in expression of proteins responsible for energy and carbohydrate metabolism, cytoskeleton, and also molecular chaperones. These proteins are the main controllers of pH and water holding capacity of meat. The pectoral muscle of broiler chickens fed E. faecium supplement had also reduced the cooking loss and drip loss (Zheng et al. 2015).

 

Effect of enterococcal probiotics on intestinal morphology in poultry

The gastrointestinal tract is responsible for the uptake of nutrients, remove pathogens and immune response. Luo et al. (2013) reported that dietary supplementation of E. faecium increased gut microvilli and influenced immune organ development and mucosal structure chickens. They also showed that dietary supplement of E. faecium had a pronounced effect on genes expression related to the intestinal tissue development and epithelium maturation, and genesresponsible for digestion and absorption of nutrients. The mucin is a very glycosylated protein that is synthesized by the goblet cell in epithelial tissues and acts as a protective barrier (Marin et al. 2008). In the study of Luo et al. (2013), E. faecium supplementation led to down-regulation of mucin-2 which is a member of mucin protein family, which can combine to pathogens as part of the immune response (Johansson et al. 2011). Samli et al. (2007) also reported that dietary supplementation of E. faecium increased the villus height in jejunum and ileum of broilers, which could enhance the digestive and absorptive capacity of the intestinal tract because of a higher absorptive surface area, up-regulation of brush border enzymes and enhancing nutrient transport mechanisms (Amat et al. 1996). In the study of Cao et al. (2013), dietary inclusion of E. faecium increased villi height and decreased crypt depth in the jejunum and the same effect was observed using an antibiotic. Chichlowski et al. (2007) found that a multi-strain probiotic containing Lactobacilli, Thermophilum, Bifidobacterium, and E. faecium increased villus height and decreased the crypt depth in jejunum, compared with the control group or birds fed salinomycin. Therefore, it seems that dietary E. faecium probiotic could play a positive role in the small intestinal morphology of broilers.

 

Effect of enterococcal probiotics on immune responses in poultry

There have are several reports in the literature of the positive effects of enterococcal probiotics on poultry immune response. Zheng et al. (2016), suggested that the improved production efficiency in the broiler chickens fed with E. faecium supplement could be attributed to lower nutrient costs for immune response and more available nutrient for growth of birds.

 

Table 2 The effects of Enterococcus probiotic on performance of broilers

 

 

In the study of Luo et al. (2013), the relative weights of intestine, spleen and Bursa Fabricius were heavier in chickens fed E. faecium probiotic, they concluded that dietary supplementation of E. faecium could improve immune organ development. They also found that the E. faecium probiotic decreased the inflammation and oxygen stress conditions in the intestinal mucosa of broilers. This means less energy costs and probably explains the improved feed conversion ratio. In the study of Majidi-Mosleh et al. (2017), the antibody titre against Newcastle disease virus, antibody titre against sheep red blood cells and cell-mediated immune response was not influenced in E. faecium fed broilers. Cao et al. (2013), studied the pattern of immune system related gene expression in response to dietary E. faecium supplementation. They observed an up-regulation of Interleukin 4 (IL-4) which has a key role in the immune responses, in the jejunal mucosa of chicks fed E. faecium probiotic. In birds treated with E. faecium, the expression of tumor necrosis factor alpha (TNF-α), a key cytokine responsible for systemic inflammation and is one of the cell signaling proteins involved in the acute phase reaction, was also increased in the jejunal mucosa. Birds fed E. faecium probiotic had higher levels of secretory immunoglobulin A (S-IgA) in jejunal mucosa, which is an important factor in protecting organs such as oral cavity, intestine, and lungs from invading pathogens. As a matter of fact, the majority of invading pathogens makes first contacts with the host at mucosal level, particularly in the agasreo-intestinal tract, and S-IgA is known as the first protective ban (Muir et al. 1998). Phagocytic action is an important constituent of the cellular innate immunity system and has a vital role in host protection against pathogens. Laukova et al. (2017) reported that Phagocytic activity was considerably increased in chickens fed E. faecium probiotic and attributed it to the ability of E. faecium CCM8558 strain to promote the toll-like receptors (TLRs). The TLRs are pattern detection receptors that act as pathogens invading sensors and are vital for the start the innate inflammatory and adaptive immune reactions (Shang et al. 2008). There are also reports on the effects of E. faecium probiotic on up-regulation of MIF, IFN-β, MD-2, and CD14 immune system related proteins in chickens (Karaffova et al. 2017). Table 3 shows the effects of E. faecium based probiotics on immune system of broilers.

 

Effect of enterococcal probiotics on blood parameters in poultry

There are reports on the effects of probiotics in altering the chicken blood lipid fractions as an index of body metabolism (Panda et al. 2006). Capcarova et al. (2010) showed that the E. faecium M 74 probiotic reduced levels of total cholesterol and also total lipids in blood plasma. The blood cholesterol concentration is an important factor to prevent atherosclerosis, and it’s known that atherosclerosis could be controlled by adjusting the blood cholesterol level (Kapila et al. 2009). De Smet et al. (1994) found that probiotics increase the production of unconjugated bile acids, therefore involve in the regulation of blood cholesterol, which is the precursor substance of bile acids. Capcarova et al. (2008) reported that blood bilirubin concentration increased in broilers fed E. faecium M 74 and addition of a probiotc containing L. fermentum and E. faecium caused in an increase in serum calcium and iron concentration and a lower blood triglyceride level. The higher serum calcium concentration could be a positive effect for the animals to reach a more strength bone and growth rate. Capcarova et al. (2010) studied the effect of E. faecium M74 strain on blood parameters of laying hens, and found a reduced levels of calcium, lipids, cholesterol, haematocrit values and leucocyte counts in plasma, although the triglyceride level was not altered and the erythrocyte counts were greater than before.

 

Table 3 The effects of Enterococcus probiotic on immune system responses of broilers

 

 

Probiotic application was not changed the egg production parameters. The effect of E. faecium CCM8558 strain on blood lipid fractions could be attributed to the fact that the probiotic is a lactic acid producing bacteria, release bile degrading enzymes, deconjugates bile, and reduces pH. These alterations could decrease blood triglycerides or cholesterol levels (Bovdisova and Capcarova, 2015). In the experiment of Capcarova et al. (2008), supplementation of E. faecium M 74 strain in drinking water had no effect on serum hepatic enzymes, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (GGT) and glutamate dehydrogenase (GLDH). However, the antioxidant potential of E. faecium M 74 strain added was proved, such that multivalent anti-oxidativity (TAS) was increased, which is an index to inhibit the exogenous and endogenous oxidative stress.

 

CONCLUSION

To date, the Enterococcus faecium are known as safe probiotic microorganisms which enhance the immune system and performance of broiler chickens. However, various factors may affect the potential probiotic effects and more investigations may be needed to conclusively reveal the involved mechanisms.

Amat C., Planas J.M. and Moreto M. (1996). Kinetics of hexose uptake by the small and large intestine of the chicken. American J. Physiol. 271, 1085-1089.

Awad W., Ghareeb K., Abdel-Raheem S. and Böhm J. (2009). Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult. Sci. 88, 49-56.

Bovdisova I. and Capcarova M. (2015). Effect of a probiotic product on the content of cholesterol and triglycerides in the blood serum of laying hens. Pp. 42-45 in Proc. Conf. Young Sci., Nitra, Slovak Republic.

Caglar E., Kargul B. and Tanboga I. (2005). Bacteriotherapy and probiotics’ role on oral health. Oral Dis. 11, 131-137.

Cao G.T., Zeng X.F., Chen A.G., Zhou L., Zhang L., Xiao Y.P. and Yang C.M. (2013). Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poult. Sci. 92, 2949-2955.

Capcarova M., Kolesarova A., Massanyi P. and Kovacik J. (2008). Selected blood biochemical and haematological parameters in turkeys after experimental probiotic Enterococcus faecium M 74 strain administration. Int. J. Poult. Sci. 7, 1194-1199.

Capcarova M., Weiss J., Hrncar C., Kolesarova A. and Pal G. (2010). Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens. J. Anim. Physiol. Anim. Nutr. 94, 215-224.

Chichlowski M., Croom W.J., Edens F.W., MacBride B.W., Qiu R., Chiang C.C., Daniel L.R., Havenstein G.B. and Koci M.D. (2007). Microarchitecture spatial relationship between bacteria and ileal, cecal colonic in chicks fed a direct-fed microbial, PrimaLac, and salinomycin. Poult. Sci. 86, 1121-1132.

Crawford J.S. (1979). Probiotics in animal nutrition. Pp. 45-55 in Proc. Arkansas Nutr. Conf., Arkansas, USA.

Demeterová M., Mariscáková R., Pistl J., Nad P. and Samudovská A. (2009). The effect of the probiotic strain Enterococcus faecium DSM 7134 in combination with natural humic substances on performance and health of boiler chickens. Berl. Munch. Tierarztl. Wochenschr. 122, 370-377.

De Smet I., Van Hoorde L., De Saeyer M., Van De Woeslyne M. and Verstraele W. (1994). In vitro study of bile salt hydrolase (BSH) activity of BSH isogenic Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH activity. Microb. Ecol. Health Dis. 7, 315-329.

Devriese L.A., Pot B. and Collins M.D. (1993). Phenotypic identi fi cation of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J. Appl. Bacteriol. 75, 399-408.

Devriese L.A., Hommez J., Pot B. and Haesebrouck F. (1994). Identification and composition of the streptococcal and enterococcal flora of tonsils, intestines and faeces of pigs. J. Appl. Bacteriol. 77, 31-36.

Devriese L.A. and Pot B. (1995). The genus Enterococcus. Pp. 327-367 in The Lactic Acid Bacteria. : The Genera of Lactic Acid Bacteria. B.J.B. Wood and W.H. Holzapfel, Eds. Blackie Academic, London.

Devriese L.A., Hommez J., Wijfels R. and Haesebrouck F. (1991). Composition of the enterococcal and streptococcal intestinal flora of poultry. J. Appl. Bacteriol. 71, 46-50.

Devriese L., Baele M. and Butaye P. (2003). The genus Enterococcus. Pp. 101-107 in The Prokaryotes. M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer and E. Stackebrandt, Eds. Springer-Verlag Berlin Heidelberg Publisher, Germany.

Erkkila S. and Petaja E. (2000). Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Sci. 55, 297-300.

Foulquié-Moreno M.R., Sarantinopoulos P., Tsakalidou E. and De Vuyst L. (2006). The role and application of Enterococci in food and health. Int. J. Food Microbiol. 106, 1-24.

Franz C.M., Holzapfel W.H. and Stiles M.E. (1999). Enterococci at the crossroads of food science? Int. J. Food Microbiol. 47, 1-24.

Franz C.M. and Holzapfel W.H. (2006). The enterococci. Pp. 557-613 in Emerging Foodborne Pathogens. Y. Motarjemi and M. Adams, Eds. Woodhead Publishing, Sawston, United Kingdom.

Franz C.M., Huch M., Abriouel H., Holzapfel W. and Gálvez A. (2011). Enterococci as probiotics and their implications in food safety. Int. J. Food Microbiol. 151, 125-140.

Ghareeb K., Awad W.A., Mohnl M., Porta R., Biarnés M., Böhm J. and Schatzmayr G. (2012). Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens. Poult. Sci. 91, 1825-1832.

Grimes J.L., Rahimi S., Oviedo E., Sheldon B.W. and Santos F.B.O. (2008). Effects of a direct-fed microbial (Primalac) on turkey poult performance and susceptibility to oral Salmonella challenge. Poult. Sci. 87, 1464-1470.

Johansson M.E., Larsson J.M. and Hansson G.C. (2011). The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host-microbial interactions. Proc. Natl. Acad. Sci. USA. 108(1), 4659-4665.

Kacaniova M., Kmet V. and Cubon J. (2006). Effect of Enterococcus faecium on the digestive tract of poultry as a probiotic. Turkish J. Vet. Anim. Sci. 30, 291-298.

Kapila S., Vibha P. and Sinha R. (2009). Antioxidative and hypocholesterolemic effect of Lactobacillus casei ssp. Casei (biodefensive properties of Lactobacilli). Indian J. Med. Sci. 60, 361-370.

Karaffova V., Marcinkova E., Bobikova K., Herich R., Revajova V., Stasova D., Kavulova A., Levkutova M., Levkut Jr M., Laukova A., Sevcikova Z. and Levkut S.M. (2017). TLR4 and TLR21 expression, MIF, IFN-β, MID-2, CD14 activation, and sIgA production in chickens administered with EFAL41 strain challenged with Campylobacter jejuni. Folia Microbiol. 62, 89-97.

Kralik G., Milakovic Z. and Ivankovic S. (2004). Effect of probiotic supplementation on the performance and the composition of the intestinal microflora in broilers. Acta Agraria Kaposvá Riensis. 8, 23-31.

Laukova A., Guba P., Nemcova R. and Marekova M. (2004). Inhibition of Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails. Vet. Med. Czech. 49, 47-51.

Laukova A., Pogany Simonova M., Chrastinova L., Kandri-cakova A., Scerbova J., Placha I., Cobanova K., Formelova Z., Ondruska L., Strkolcova G. and Strompfova V. (2017). Beneficial effect of bacteriocin strain Enterococcus du-rans ED26E/7 in model experiment using broiler rabbits. Czech J. Anim. Sci. 62, 168-177.

Leclercq H., Devriese L.A. and Mossel D.A.A. (1996). Taxonomical changes in intestinal (faecal) Enterococci and streptococci: Consequences on their use as indicators of faecal contamination in drinking water. J. Appl. Bacteriol. 81, 459-466.

Lee N.K., Yun C.W., Kim S.W., Chang H.I., Kang C.W. and Paik H.D. (2008). Screening of Lactobacilli derived from chicken feces and partial characterization of Lactobacillus acidophilus A12 as an animal probiotics. J. Microbiol. Biotechnol. 18, 338-342.

Levkut M., Pustl J., Lauková A., Revajova V., Herich R., Ševcíková Z., Strompfova V., Szaboova R. and Kokincakova T. (2009). Antimicrobial activity of Enterococcus faecium 55 against Salmonella enteritidis in chicks. Acta Vet. Hung. 57, 13-24.

Liong M.T. and Shah N.P. (2005). Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. J. Dairy Sci. 88, 55-66.

Luo J., Zheng A., Meng K., Chang W., Bai Y., Li K., Cai H., Liu G. and Yao B. (2013). Proteome changes in the intestinal mucosa of broiler (Gallus gallus) activated by probiotic Enterococcus faecium. J. Proteomics. 91, 226-241.

Majidi-Mosleh A., Sadeghi A., Mousavi S.N., Chamani M. and Zarei A. (2017). Ileal MUC2 gene expression and microbial population, but not growth performance and immune response, are influenced by in ovo injection of probiotics in broiler chickens. British Poult. Sci. 58, 40-45

Marin F., Luquet G., Mari B. and Medakovic D. (2008). Molluscan shell proteins: primary structure, origin, and evolution. Curr. Top. Dev. Biol. 80, 209-276.

Mitsuoka T. (2002). Research in intestinal flora and functional foods. J. Int. Microbiol. 15, 57-89.

Mountzouris K.C., Tsistsikos P., Kalamara E., Nitsh S., Schatzmayr G. and Fegeros K. (2007). Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modualting cecal microflora composition and metabolic activities. Poult. Sci. 86, 309-317.

Muir W.I., Bryden W.L. and Husbandw A.J. (1998). Evaluation of the efficacy of intraperitoneal immunization in reducing Salmonella typhimurium infection in chickens. Poult. Sci. 77, 1874-1883.

Panda A.K., Ramarao S.V., Raju M.V.L.N. and Sharma S.R. (2006). Dietary supplementation of Lactobacillus sporogenes on performance and serumbiochemico-lipid profile of broiler chickens. J. Polt. Sci. 43, 235-240.

Samli H.E., Senkoylu N., Koc F., Kanter M. and Agma A. (2007). Effects of Enterococcus faecium and dried whey on broiler perforance, gut histomorphology and intestinal microbiota. Arch. Anim. Nutr. 61, 42-49.

Samli H.E., Dezcan S., Koc F., Ozduven M.L., Okur A.A. and Senkoylu N. (2010). Effects of Enterococcus faecium supplementation and floor type on performance, morphology of erythrocytes and intestinal microbiota in broiler chickens. British Poult. Sci. 51, 564-568.

Schleifer K.H. and Kilpper-Bälz R. (1984). Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nomrev as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int. J. Syst. Bacteriol. 34, 31-34.

Shang L., Fukata M., Thirunarayanan N., Martin A.P., Arnaboldi P., Maussag D., Berin C., Unkeless J.C., Mayer L., Abreu M.T. and Lira S.A. (2008). TLR signaling in small intestinal epithelium promotes B cell recruitment and IgA production in lamina propria. Gastroenterology. 135, 529-538.

Sorum H. and Sunde M. (2001). Resistance to antibiotics in the normal flora of animals. Vet. Res. 32, 227-241.

Vahjen W., Jadamus A. and Simon O. (2002). Influence of probiotic Enterococcus faecium strain on selected bacterial groups in the small intestine of growing turkey poults. Arch. Anim. Nutr. 56, 419-429.

Willis W.L. and Reid L. (2008). Investigating the effects of dietary probiotic feeding regimens on broiler chicken production and Campylobacter jejuni presence. Poult. Sci. 87, 606-611.

Zhao X., Guo Y., Guo S. and Tan J. (2013). Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler chickens. Appl. Microbiol. Biotechnol. 97, 6477-6488.

Zheng A., Luo J., Meng K., Li J., Zhang S., Li K., Liu G., Cai H., Bryden W.L. and Yao B. (2015). Proteome changes underpin improved meat quality and yield of chickens (Gallus gallus) fed the probiotic Enterococcus faecium. BMC Genomics. 15, 1167-1171.

Zheng A., Luo J., Meng K., Li J., Bryden W.L., Chang W., Zhang S., Wang L.X.N., Liu G. and Yao B. (2016). Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus). BMC Genomics. 17, 89-97.