1Department of Animal Science, Faculty of Agricultural Science, Lorestan University, Khoramabad, Iran
2Department of Veterinary Science, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran
Receive Date: 14 October 2015,
Revise Date: 12 December 2015,
Accept Date: 15 December 2015
Eggs of the indigenous laying hens are preferred by consumers to those obtained from commercial breeds. The present study aimed to compare the fatty acid (FA) composition of yolk lipids of indigenous eggs (Indigenous; n = 20) and conventional eggs (Conventional; n = 20). Indigenous and conventional eggs were collected from Lori layer hens reared under free-range condition and an commercial farm respectively. The FA composition of yolk lipids in indigenous and conventional eggs was determined by gas chromatography. Lori eggs (48.7 g) were significantly lighter than conventional eggs (59.6 g). Total fatty acid content (g/100 g fresh yolk) was similar in Lori and conventional egg yolk with 27.7 and 28.6 respectively. Lori eggs had significantly higher docosahexaenoic (DHA, C22:6n3), α-linoleic (ALA, C18:3n3) and oleic acid (C18:1n9cis) levels and lower linoleic (C18:2n6cis) levels than conventional eggs. Lori eggs had significantly higher content of n-3 and lower content of n-6 FA than the conventional eggs. In conclusion, the n-6/n-3 ratio in Lori eggs (8.1) was significantly lower than that in conventional eggs (17.4) proposing that indigenous eggs may be healthier for the consumers than the conventional eggs.
Rural Indigenous chicken production in Iran goes back a long way (Shariatmadari, 2000; Vali, 2008). Local poultry production plays an important role in livelihood of the farmers not only for their products (meat, eggs) but also for improving farmers’ income (Mwalusanya et al. 2002). Indigenous laying hens are usually kept in free-range condition and they graze mainly green plants and scavenge for earthworms (Mwalusanya et al. 2002). It has been previously reported that FA composition of eggs was affected by laying hens diet (Beynen, 2004; Milinsk et al. 2003), farming conditions (Rizzi et al. 2007) and housing system (Hidalgo et al. 2008; Lopez-Bote et al. 1998). Furthermore, studies have shown that FA composition of the egg was also influenced by the laying hen breed (Millet et al. 2006; Scheideler et al. 1998). Eggs of indigenous laying hens differ from that of commercial hens in term of breed, housing condition, and diets. Consumers mainly prefer eggs of the indigenous laying hens rather than those obtained from commercial breeds. While fatty acid composition of conventional eggs is well studied (Cherian et al. 2002; Samman et al. 2009), data regarding the FA composition of eggs produced by local indigenous breeds are limited. The Lori laying hen is an indigenous breed reared by villagers in the west of Iran, mainly in Lorestan province. The Lori layer hen is a farmyard breed and rears under free-range conditions. The Lori hen produces about 80 to 100 brown shell eggs. In spite of favorability of Lori eggs for the consumers, actual FA composition of Lori eggs has not yet been determined. Thus, the objectives of this study were to determine the FA composition and to compare n-6/n-3 ratio of yolk lipids of indigenous Lori eggs to that of the conventional eggs.
MATERIALS AND METHODS
Sample collection and preparation
Indigenous eggs were collected from Lori layer hens kept under free-range condition in a village near Khorramabad city, Iran. It is a semi-dried area with average annual rainfall of about 472 mm, an altitude of 1125 m and average temperature of 17.3 ˚C. The Lori hens grazed fresh green grass and legumes ad libitum. The predominant plant species of the ranges were ryegrass (Lolium perenne) and clover (Trifolium spp.). Lori hens habitually scavenge earthworms. Conventional eggs were collected from an industrial laying hen farm (Leghorn breed). In total, 40 eggs were collected (20 Lori eggs and 20 commercial Lohmann selected Leghorn eggs). All eggs were weighed and the yolks were separated from the whites and frozen at −20 ˚C pending analysis.
Lipid extraction and FA methylation
Fatty acid methyl esters (FAME) were determined using the procedures described by Sukhija and Palmquist (1988) with some modification. Briefly, about 0.1 g yolk was weighed by digital scale (0.0001, KERN, Germany) and then was dried by means of freeze dryer for 48 h. Freeze-dried samples were weighed again after drying and were placed into culture tube (16×125 mL, Scott glass tube). About one mL heptane including internal standard (C13:0) was added and mixed. Then 0.2 mL of sodium methylate (25%) was added and the tube was put in a 50 ˚C water bath for 10 min. After that, sample was cooled for about 5 min. Then 3 mL of freshly made methanolic HCl 10% (prepared by adding 20 mL of acetyl chloride to 100 mL of anhydrous methanol) was added and vortexed. The tube was put in a 90 ˚C steam bath for 30 min and then the tube sample was cooled. Finally, one mL of heptane and three mL of potassium carbonate 10% was added and mixed for one min. The sample was centrifuged (Centrifuge 5415 R; Rotofix 32A, Germany) for 5 min at 1500 g, and Heptane phase (upper phase) and was transferred to the GC vial (1.5 mL) using a pastor pipette.
Determination of FA composition
FAME were analyzed by gas chromatography with flame ionization detection (GC-FID; HP 6890 chromatograph, Hewlett-Packard, Avondale, PA, USA) using a Chrompack CP-Sil 88 TM fused silica capillary column (100 m×0.25 mm internal diameter , 0.2 mm film thickness; Varian Inc., Walnut Creek, CA, USA). Briefly, the oven temperature was initially 150 ˚C (held for 5 min), then increased for 5 ˚C/min to 180 ˚C (held for 30 min), then increased by 1 ˚C/min to 190 ˚C (held for 5 min) and finally increased by1 ˚C/min to 200 ˚C (held for 35 min). Hydrogen was used as the carrier gas at a flow rate of 1.0 mL/min. The injector and detector temperatures were maintained at 280 and 300 ˚C respectively. Identification of common FA was accomplished by comparison of sample peak retention times with those of FAME standard mixtures (SupelcoTM 37 component FAME Mix, Supelco-47885-U, Sigma-Aldrich Chemie GmbH, Germany). Quantification of total FAME was calculated using tridecanoic acid (C13:0) as internal standard (Fluka-91988, Sigma-Aldrich Chemie GmbH, Germany). The FA content of yolk egg was calculated as concentration (mg/g)= peak area of a given FA × concentration of internal standard (mg/mL) / peak area of internal standard / sample weight (g).
Data were analyzed by SAS/STAT software (SAS, 2001) using t-test procedure. Normality of residuals was tested using a Shapiro-Wilk test. Statistical differences with (P<0.05) were considered significant.
RESULTS AND DISCUSSION
The weight of indigenous Lori eggs were lower compared to the conventional white Leghorn eggs (48.7±3.6 vs. 59.6±3.2, P<0.01). The weight of the Lori eggs was in line with the reported range of eggs weigh for the other Iranian indigenous laying hens (Vali, 2008). In agreement with the finding of the present study, Araucana (a fancy breed) hens produced lighter egg compared to the commercial breeds (Millet et al. 2006). Basically, eggs weight is determined by laying hen breed, feed intake, and housing conditions (Hughes etal. 1985; Millet et al. 2006). Thus, the lower weight of indigenous eggs compared to the conventional eggs could be partly explained by feed intake and the type of breed. The FA compositions of indigenous Lori and conventional egg yolks both as percentage (wt.%) and as content (g) in 100 g fresh yolk are shown in Table 1 and Table 2 respectively. In Lori eggs similar to the commercial eggs, palmitic acid (C16:0) and stearic acid (C18:0) were the predominant saturated FA, which was in agreement with the previously reported values (Hidalgo et al. 2008; Samman et al. 2009; Simčič et al. 2011). Lori egg yolk had about 20% higher stearic acid (C18:0) than the conventional egg (9.98 vs. 8.43 wt.%) albeit total saturated FA (wt. %) did not differ between two type of eggs. The higher stearic acid in indigenous egg might be due the fact that Lori hens consumed animal fats with high content of stearic acid found in worms (Hansen and Czochanska, 1975) and insects (Raksakantong et al. 2010).
Table 1 Fatty acid composition of yolk (wt. %) of indigenous Lori and white Leghorn hens reared under indigenous and conventional methods respectively
n: number of samples and SD: standard division.
* (P<0.05); ** (P<0.01) and *** (P<0.001).
NS: non significant.
Table 2 Fatty acid content of yolk egg (g/100 g yolk) of indigenous Lori and white Leghorn hens reared under indigenous and conventional methods respectively
n: number of samples and SD: standard division.
* (P<0.05); ** (P<0.01) and *** (P<0.001).
NS: non significant.
The higher stearic acid in Lori eggs compared to the conventional eggs could have health beneficial effects for the consumers, because steraic acid has a neutral effect on diet-related disease risk (Baum, et al. 2012). Arachidonic acid (C20:4 n-6) and docosahexanoic acid (C22:6 n-3) were the most abundant long chain unsaturated FA in both indigenous and conventional eggs. Lori egg yolks had significantly higher α-linolenic acid (ALA, C18:3 n-3) and higher docosahexanoic acid (DHA, C22:6 n-3) but lower linoleic acid (LA, C18:2 n-6) than the conventional egg yolks. When values are presented as g per 100 g fresh yolk, Lori yolk eggs had twice much as high docosahexanoic acid (C22:6 n-3, 250 vs. 150 mg/100 g yolk) and relatively higher α-linolenic acid (C18:3 n-3) compared to the conventional eggs. On the other hand, conventional eggs had higher linoleic acid (C18:2 n-6). Consequently, Lori egg yolks had higher n-3 FA (400 vs. 200 mg/100g yolk) levels but lower n-6 FA (3.3 vs. 4.3 g/100 g yolk) levels than the conventional egg yolks. In agreement with the present results, eggs from Araucana compared to commercial Lohmann selected Leghorn had about 120 mg/100 g yolk higher docosahexanoic acid (Millet et al. 2006). Higher values of n-3 fatty acids found in Lori yolk compared to the conventional eggs might be due to the fact that Lori laying hens grazed on fresh green grass and legumes. Fresh green plants contain higher proportion of polyunsaturated FA especially α-linoleic acid than the commercial mixed diet (Lopez-Bote et al. 1998). The eggs of white Leghorn laying hens fed with grass and a commercial mixed diet under free-range conditions had more n-3 FA as well as α-Linolenic acid and docosahexanoic acid than the eggs of the hens fed only with a commercial diet (Lopez-Bote et al. 1998). Interestingly, the n-6/n-3 ratio in the Lori eggs (8.1) was lower than that in the conventional eggs (17.4). The lower ratio of n-6/n-3 in Lori egg compared to the value in commercial egg is considered healthier from the nutritional point of view. In the present study, the n-6/n-3 ratio found in the conventional egg was in good agreement with previously reported value for market egg (Samman et al. 2009). The n-6/n-3 ratio in human nutrition is important for human health (Simopoulos, 2008). A lower n-6/n-3 ratio of FA is desirable due to the links between unbalanced n-6/n-3 ratio and prevalence of many of the human chronic diseases (Simopoulos, 2008). The evidence have shown that there are relations between unbalanced n-6/n-3 ratio and cardiovascular disease, cancer, insulin resistance, depression, early aging, diabetes, and obesity (Adkins and Kelley, 2010; Bourre, 2004; Simopoulos, 2008). In recent decades, n-6 FA consumption had increased by 250%, whilen-3 FA had decreased by 30% in the human diets (Kouba and Mourot, 2011). Findings of the present study revealed that indigenous Lori layer hens produce eggs with healthier n-6/n-3 ratio compared to market eggs (commercial leghorn eggs). However, it should be mentioned that again the good nutritional characteristics of indigenous eggs might be due to the hens’ diet rather than the hens’ breed. In conventional production system, hens are fed with formulated mixed feeds, whereas indigenous breeds had free access to the green forages. Basically, green forages have high content of LA (7 to 37 g), ALA (2 to 10 g) and palmitic acid (3 to 8 g) (Clapham et al. 2005) and most likely, secondary metabolites in legumes may affect FA metabolism. Karsten et al. (2010) reported that eggs of pastured hens had twice as much n-3 fatty acids, and 2.5 fold more total n-3 fatty acids compared to eggs of caged hens (Karsten et al. 2010). Further research is needed to determine whether the healthy n-6/n-3 ratio found in indigenous eggs was due to the laying hens’ diets or to the breed per se.
This study showed that eggs produced by indigenous Lori layers reared under free-range condition had higher stearic acid and higher n-3 fatty acids compared to the market eggs. The n-6/n-3 ratio in Lori eggs (8.1) was significantly lower than that in conventional eggs (17.4) proposing that indigenous eggs may be healthier for the consumers than the conventional eggs.
This study was totally sponsored (grant number 9060285) by Research Deputy of Lorestan University.
Adkins Y. and Kelley D.S. (2010). Mechanisms underlying the cardioprotective effects of omega-3 polyunsaturated fatty acids. J. Nutr. Biochem.21, 781-792.
Baum S.J., Kris-Etherton P.M., Willett W.C., Lichtenstein A.H., Rudel L.L., Maki K.C., Whelan J., Ramsden C.E. and Block R.C. (2012). Fatty acids in cardiovascular health and disease: a comprehensive update. J. Clin. Lipidol.6, 216-234.
Beynen A.C. (2004). Fatty acid composition of eggs produced by hens fed diets containing groundnut, soya bean or linseed. NJAS-Wagen. J. Life Sci.52, 3-10.
Bourre J.M. (2004). Roles of unsaturated fatty acids (especially omega-3 fatty acids) in the brain at various ages and during ageing. J. Nutr. Health Aging. 8, 163-174.
Cherian G., Holsonbake T.B. and Goeger M.P. (2002). Fatty acid composition and egg components of specialty eggs. Poult. Sci.81, 30-33.
Clapham W.M., Foster J.G., Neel J.P.S. and Fedders J.M. (2005). Fatty acid composition of traditional and novel forages. J. Agric. Food Chem.53, 10068-10073.
Hansen R.P., and Czochanska Z. (1975). The fatty acid composition of the lipids of earthworms. J. Sci. Food Agric. 26, 961-971.
Hidalgo A., Rossi M., Clerici F. and Ratti S. (2008). A market study on the quality characteristics of eggs from different housing systems. Food Chem. 106, 1031-1038.
Hughes B.O., Dun P. and McCorquodale C.C. (1985). Shell strength of eggs from medium-bodied hybrid hens housed in cages or on range in outside pens. Br. Poult. Sci.26, 129-136.
Karsten H.D., Patterson P.H., Stout R. and Crews G. (2010). Vitamins A, E and fatty acid composition of the eggs of caged hens and pastured hens. Renew. Agric. Food Syst. 25, 45-54.
Kouba M. and Mourot J. (2011). A review of nutritional effects on fat composition of animal products with special emphasis on n-3 polyunsaturated fatty acids. Biochimie. 93, 13-17.
Lopez-Bote C.J., Sanz Arias R., Rey A.I., Castano A., Isabel B. and Thos J. (1998). Effect of free-range feeding on n-3 fatty acid and α-tocopherol content and oxidative stability of eggs. Anim. Feed Sci. Technol.72(1), 33-40.
Milinsk M.C., Murakami A.E., Gomes S.T.M., Matsushita M. and de Souza N.E. (2003). Fatty acid profile of eggs yolk lipids from hens fed diets rich in n-3 fatty acids. Food Chem. 83(2), 287-292.
Millet S., De Ceulaer K., Van Paemel M., Raes K., De Smet S. and Janssens G.P. (2006). Lipid profile in eggs of Araucana hens compared with Lohmann Selected Leghorn and ISA Brown hens given diets with different fat sources. Br. Poult. Sci. 47(3), 294-300.
Mwalusanya N.A., Katule A.M., Mutayoba S.K., Mtambo M.M.A., Olsen J.E. and Minga U.M. (2002). Productivity of local chickens under village management conditions. Trop. Anim. Health Prod. 34, 405-416.
Raksakantong P., Meeso N., Kubola J. and Siriamornpun S. (2010). Fatty acids and proximate composition of eight Thai edible terricolous insects. Food Res. Int. 43, 350-355.
Rizzi C., Marangon A. and Chiericato G.M. (2007). Effect of genotype on slaughtering performance and meat physical and sensory characteristics of organic laying hens. Poult. Sci. 86, 128-135.
Samman S., Kung F.P., Carter L.M., Foster M.J., Ahmad Z.I., Phuyal J.L. and Petocz P. (2009). Fatty acid composition of certified organic, conventional and omega-3 eggs. Food Chem. 116, 911-914.
SAS Institute. (2001). SAS®/STAT Software, Release 8.2. SAS Institute, Inc., Cary, NC. USA.
Scheideler S.E., Jaroni D. and Froning G. (1998). Strain and age effects on egg composition from hens fed diets rich in n-3 fatty acids. Poult. Sci. 77, 192-196.
Shariatmadari F. (2000). Poultry production and the industry in Iran. World's Poult. Sci. J. 56, 55-65.
Simčič M., Stibilj V. and Holcman A. (2011). Fatty acid composition of eggs produced by the Slovenian autochthonous Styrian hen. Food Chem. 125, 873-877.
Simopoulos A.P. (2008). The importance of the omega-6 / omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp. Biol. Med. 233, 674-688.
Sukhija P.S. and Palmquist D.L. (1988). Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. J. Agric. Food Chem.36(6), 1202-1206.
Vali N. (2008). Indigenous chicken production in Iran: a review. Pakistan J. Biol. Sci. 11, 2525-2531.