Effects of Calcium, Phosphorus and Zinc in Wheat-Based Diets on Broiler Chickens’ Performance, Immunity and Bone Parameters

Document Type : Research Article


Department of Animal Science, University of Ilam, Ilam, Iran


An experiment was conducted to investigate the effect of zinc (Zn) supplementation and different concentrations of calcium (Ca) and phosphorus (P) in wheat-based diets on the performance, immune responses and bone parameters of broiler chickens. A randomized complete block design with factorial arrangement was used (three concentrations of Zn supplementation×two concentrations of dietary Ca-P), 300 day-old broilers were assigned to six dietary treatments with five replicates of ten birds. Dietary treatments were the basal diet (control; TRT1), control plus 50 ppm Zn (TRT2), control plus 70 ppm Zn (TRT3), low Ca-P diet (0.60 to 0.30%; TRT4), low Ca-P diet plus 50 ppm Zn (TRT5) and low Ca-P diet plus 50 ppm Zn (TRT6). Ca and P in the control diet were 0.90 and 0.45% in the grower phase and 0.85 and 0.42% in the finisher phase. Changes in dietary Ca-P had no effect on body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR) or serum Ca and P concentrations (P>0.05) whereas Zn supplementation increased FI (P<0.05). The addition of 50 ppm Zn increased serum P concentration (P<0.05) and dietary treatments had no effect on antibody titers against sheep red blood cells (SRBC) (P>0.05). The lowest blood heterophil (H) and the highest lymphocyte (L) percentages and lowest H:L ratio were observed in birds fed with the diet containing a standard Ca-P with 70 ppm Zn supplementation (P<0.05). Dietary treatments had no effect on bone length, thickness and breaking strength (P>0.05). Tibia and fibula ash decreased by feeding lower Ca-P than the standard diet (P<0.05). It is concluded that low Ca-P diets did not have a detrimental effect on performance or blood and bone parameters and that Zn supplementation did not improve those parameters when feed was low in Ca-P.


AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Bao Y., Choct M., Iji P. and Bruerton K. (2009). Optimal dietary inclusion of organically complexed zinc for broiler chickens. Br. Poult. Sci. 50, 95-102.
Barr V.A., Malide D., Zarnowski M.J., Taylor S.I. and Cushman S.W. (1997). Insulin stimulates both leptin secretion and production by rat white adipose tissue. Endocrinology. 138, 4463-4472.
Boskey A., Wright T. and Blank R. (1999). Collagen and bone strength. J. Bone Min. Res. 14, 330-335.
Brandão-Neto J., Stefan V., Mendonça B.B., Bloise W. and Castro A.V.B. (1995). The essential role of zinc in growth. Nutr. Res. 15, 335-358.
Burrell A., Dozier W., Davis A., Compton M., Freeman M., Vendrell P. and Ward T. (2004). Responses of broilers to dietary zinc concentrations and sources in relation to environmental implications. Br. Poult. Sci. 45, 225-263.
Cheema M., Qureshi M. and Havenstein G. (2003). A comparison of the immune response of a 2001 commercial broiler with a 1957 randombred broiler strain when fed representative 1957 and 2001 broiler diets. Poult. Sci. 82, 1519-1529.
Delhanty J. and Solomon J. (1966). The nature of antibodies to goat erythrocytes in the developing chicken. Immunology. 11, 103-105.
Dołęgowska B., Machoy Z. and Chlubek D. (2003). Changes in the content of zinc and fluoride during growth of the femur in chicken. Biolog. Trace Element. Res. 91, 67-76.
Harland B. and Oberleas D. (1999). Phytic acid complex in feed ingredients. Pp. 69-76 in Phytase in Animal Nutrition and Waste Management-a BASF Reference Manual. M.B. Coelho and E.T. Kornegay, Eds. BASF Corp., Mount Olive.
Hoehler D., Lemme A., Ravindran V., Bryden W. and Rostagno H. (2005). Feed formulation in broiler chickens based on standardized ileal amino acid digestibility. Pp. 78-91 in Proc. 3rd Mid-Atlantic Nutr. Conference.
Kennedy K.J., Rains T.M. and Shay N.F. (1998). Zinc deficiency changes preferred macronutrient intake in subpopulations of Sprague-Dawley outbred rats and reduces hepatic pyruvate kinase gene expression. J. Nutr. 128, 43-49.
Kidd M., Anthony N. and Lee S. (1992). Progeny performance when dams and chicks are fed supplemental zinc. Poult. Sci. 71, 1201-1206.
Kidd M., Ferket P. and Qureshi M. (1996). Zinc metabolism with special reference to its role in immunity. World's Poult. Sci. J. 52, 309-324.
Kidd M., Anthony N., Newberry L. and Lee S. (1993). Effect of supplemental zinc in either a corn-soybean or a milo and corn-soybean meal diet on the performance of young broiler breeders and their progeny. Poult. Sci. 72, 1492-1499.
Kidd M., Qureshi M., Ferket P. and Thomas L. (2000). Turkey hen zinc source affects progeny immunity and disease resistance. J. Appl. Poult. Res. 9, 414-423.
Lee R.G., Rains T.M., Tovar-Palacio C., Beverly J.L. and Shay
      N.F. (1998). Zinc deficiency increases hypothalamic neuropeptide Y and neuropeptide Y mRNA levels and does not block neuropeptide Y–induced feeding in rats. J. Nutr. 128, 1218-1223.
Liu B.L., Rafiq A., Tzeng Y.M. and Rob A. (1998). The induction and characterization of phytase and beyond. Enzyme Microb. Tech. 22, 415-424.
Mangian H.F., Lee R.G., Paul G.L., Emmert J.L. and Shay N.F. (1998). Zinc deficiency suppresses plasma leptin concentrations in rats. J. Nutr. Biochem. 9, 47-51.
Mocchegiani E., Muzzioli M. and Giacconi R. (2000). Zinc and immunoresistance to infection in aging: new biological tools. Trend. Pharmacol. Sci. 21, 205-208.
Moran E. and Todd M. (1994). Continuous submarginal phosphorus with broilers and the effect of preslaughter transportation: Carcass defects, further-processing yields, and tibia-femur integrity. Poult. Sci. 73, 1448-1457.
NRC. (1994). Nutrient Requirements of Poultry, National Research Council. National Academy Press Washington, USA.
O'Dell B.L. (1992). Zinc plays both structural and catalytic roles in metalloproteins. Nutr. Rev. 50, 48-50.
Ott E.S. and Shay N.F. (2001). Zinc deficiency reduces leptin gene expression and leptin secretion in rat adipocytes. Exp. Biol. Med. 226, 841-846.
Pallauf J. and Rimbach G. (1997). Nutritional significance of phytic acid and phytase. Arch. Anim. Nutr. 50, 301-319.
Qureshi M. and Havenstein G. (1994). A comparison of the immune performance of a 1991 commercial broiler with a 1957 randombred strain when fed “typical” 1957 and 1991 broiler diets. Poult. Sci. 73, 1805-1812.
Rama Rao S., Raju M., Reddy M. and Pavani P. (2006). Interaction between dietary calcium and non-phytate phosphorus levels on growth, bone mineralization and mineral excretion in commercial broilers. Anim. Feed Sci. Technol. 131, 135-150.
Ravindran V., Bryden W. and Kornegay E. (1995). Phytates: occurrence, bioavailability and implications in poultry nutrition. Poult. Avian. Biol. Rev. 6, 125-143.
Ravindran V., Cadogan D., Cabahug M., Bryden W. and Selle P. (1999). Effects of phytic acid on the performance of poultry and swine. Pp. 93-99 in Phytase in Animal Nutrition and Waste Management: A BASF Reference. M.B. Coelho and E.T. Kornegay, Eds. BASF Corp., Mount Olive.
Sadoval M., Henry P., Littell R., Miles R., Butcher G. and Ammerman C. (1999). Effect of dietary zinc source and method of oral administration on performance and tissue trace mineral concentration of broiler chicks. J. Anim. Sci. 77, 1788-1799.
Sahin K. and Kucuk O. (2003). Zinc supplementation alleviates heat stress in laying Japanese quail. J. Nutr. 133, 2808-2811.
SAS Institute. (2001). SAS/WATTM User’s Guide. SAS Institute, Inc., Cary NC.
Sebastian S., Touchburn S., Chavez E. and Lague P. (1996). Efficacy of supplemental microbial phytase at different dietary calcium levels on growth performance and mineral utilization of broiler chickens. Poult. Sci. 75, 1516-1523.
Selvais P.L., Labuche C., Ninh N.X., Ketelslegers J.M., Denef J.F. and Maiter D.M. (1997). Cyclic feeding behaviour and changes in hypothalamic galanin and neuropeptide Y gene expression induced by zinc deficiency in the rat. J. Neuroendocrinol. 9, 55-62.
Sohail S. and Roland D. (1999). Influence of supplemental phytase on performance of broilers four to six weeks of age. Poult. Sci. 78, 550-555.
Sunder G.S., Panda A., Gopinath N., Rao S.R., Raju M., Reddy M. and Kumar C.V. (2008). Effects of higher levels of zinc supplementation on performance, mineral availability and immune competence in broiler chickens. J. Appl. Poult. Res. 17, 79-86.
Thorp B. and Waddington D. (1997). Relationships between the bone pathologies, ash and mineral content of long bones in 35-day-old broiler chickens. Res. Vet. Sci. 62, 67-73.
Williams B., Solomon S., Waddington D., Thorp B. and Farquharson C. (2000). Skeletal development in the meat-type chicken. Br. Poult. Sci. 41, 141-149.