Effect of Goat Follicular Fluid on in vitro Production of Embryos in Black Bengal Goats

Document Type: Research Articles

Authors

1 Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

2 Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

3 Department of Animal Breeding and Genetics, Patuakhali Science and TechnologyUniversity, Barisal, 8210, Bangladesh

4 Patuakhali Science and Technology University, Patuakhali, 8210, Bangladesh

Abstract

The study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gFF) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in Black Bengal goats. Follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. Media were prepared using TCM-199 supplemented with 0.5% bovine serum albomin (BSA) plus four levels of gFF at concentrations of 0%, 5%, 10% and 15%. Oocytes were matured for 27 h, fertilized with capacitated fresh semen in Brackett and Oliphant (BO) medium for 6 h and then cultured up to 7 days, at 38.5 ˚C with 5% CO2 under humidified air. It was observed with 0% to 15% of gFF that 53.8-75.0% of the oocytes reached the cumulus cell expansion level-3; 41.5-67.8% reached metaphase-II; 28.6-38.4% exhibited normal fertilization (formation of 2-pronuclei); 12.3-33.7% were 2-cell embryos. The development of embryos was arrested at the 2-cell stage in control media and at the 8-cell stage in 5% media, though morula and blastocyst stages developed in 10% (14.1% and 9.3%, respectively) and 15% gFF media (18.0% and 10.0%, respectively). In comparison, it was observed that the results in all stages of in vitro production of embryos could be significantly increased (P<0.05) by adding 5% gFF to control media. The results could further be improved (P<0.05) by increasing the level of gFF to 10% but no further increment (P>0.05) occurred when gFF increased to 15% level. It is concluded that gFF has a positive effect on in vitro production of embryos in Black Bengal goats and a 10% level of gFF is recommended based on the improvements observed and the associated economic benefits.

Keywords


Alia A., Coenena K., Daniel B. and Marc-Andre S. (2004). Origin of bovine follicular fluid and its effect during in vitro maturation on the developmental competence of bovine oocytes. Theriogenology. 62, 1596-1606.
Avery B., Strobech L., Jacobsen T., Bogh I.B. and Greve T. (2003). In vitro maturation of bovine cumulus-oocyte complexes in undiluted follicular fluid, effect on nuclear maturation, pronucleus formation and embryo development. Theriogenology. 59, 987-999.
Bogh I.B., Bezard J., Duchamp G., Baltsen M.,Gerard N., Daels P. and Greve T. (2002). Pure preovulatory follicular fluid promotes in vitro maturation of in vivo aspirated equine oocytes. Theriogenology. 57, 1765-1779.
Brackett B.G., Bousquet D., Boice M.L., Donawick W.J., Evans J.F. and Dressel M.A. (1982). Normal development following in vitro fertilization in the cow. Biol. Reprod. 27, 147-158.
Cha K.Y., Koo J.J., Choi D.H., Han S.Y. and Yoon T.K. (1991). Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil. Steril. 55, 109-113.
Chi H.J., Kim D.H., Koo J.J. and Chang S.S. (1998). The suitability and efficacy of human follicular fluid as a protein supplement in human in vitro fertilization programs. Fertil. Steril. 70, 871-877.
Cognié Y., Baril G., Poulin N. and Mermillod P. (2003). Current status of embryo technologies in sheep and goat. Theriogenology. 59, 171-188.
Cognié Y., Poulin N., Locatelli Y. and Mermillod P. (2004). State-of-the-art production, conservation and transfer of in vitro produced embryos in small ruminants. Reprod. Fertil. Dev. 16, 437-445.
Driancourt M.A. and Thuel B. (1998). Control of oocyte growth and maturation by follicular cells and molecules present in follicular fluid. A review.Reprod. Nutr. Dev. 38, 345-362.
Edwards R.G. (1965). Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature. 208, 349-351.
Ferdous J. (2006). Collection, grading and evaluation of goat cumulus-oocyte-complexes (COCs) in view of in vitro maturation, fertilization and culture. MS. Thesis.Department of Animal Breeding and Genetics. BangladeshAgricultural University, Mymensingh.
FukuiY. and Ono H. (1989). Effects of sera, hormones and granulosa cells added to culture medium for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil. 86, 501-506.
GardnerD.K., Lane M., Spitzer A. and Batt P.A. (1994). Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells, amino acids, vitamins, and culturing embryos in groups stimulate development. Biol. Reprod. 50, 390-400.
Goel A.K. and Agrawal K.P. (2000). Distribution pattern of estrous in Jakhrana goats. Indian J. Anim. Sci. 70, 589-590.
Islam M.R., Khandoker M.A.M.Y., Afroz S., Rahman M.G.M. and KhanR.I.(2007). Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. J. Zhejiang. Univ. 8, 465-469.
Ito M., Iwata H., Kitagawa M., Kon Y., Kuwayama T. and Monji Y. (2008). Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes. Anim. Reprod. Sci. 106, 421-430.
Jiménez-Macedo A.R., Izquierdo D., Anguita B. and Paramio M.T. (2005). Comparison between intracytoplasmic sperm injection and in vitro fertilization employing oocytes derived from prepubertal goats. Theriogenology. 64, 1249-1262.
Jiménez-Macedo A.R., Paramio M.T., Anguita B., Morato R. and Romaguera R. (2007). Effect of ICSI and embryo biopsy on embryo development and apoptosis according to oocyte diameter in prepubertal goats. Theriogenology. 67, 1399-1408.
John C.M., Dumoulin E.C., Marijke B., Lucie C.P., Van W., Rosie I.V., Jansen J.M.B., Josien G.D., Joep P.M.G. and Johannes L.H.E. (2000). Comparison of in vitro development of embryos originating from either conventional in vitro fertilization or intracytoplasmic sperm injection. Hum. Reprod. 15(2), 402-409.
Khandoker M.A.M.Y., Imai K., Takahashi T. and Hashizume K. (2001). Role of gelatinase on follicular atresia in the bovine ovary. Biol. Reprod. 65, 720-732.
Kharche S.D., Goel A.K., Jindal S.K. and Sinha N.K. (2006). In vitro maturation of caprine oocytes in different concentrations of estrous goat serum. Small Rumin. Res. 64, 186-189.
Kharche S.D., Goel A.K., Jindal S.K., Yadav E.N., Yadav P., Sinha R. and Sinha N.K. (2009). Effect of serum albumin supplementation on in vitro capacitation and fertilization of caprine oocytes. Small Rumin. Res. 81,85-89.
Kim K.S., Mitsumizo N., Fujita K. and Utsumi K. (1996). The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos. Theriogenology. 45,787-799.
Knight P.G., Muttukrishna S. and Groome N.P. (1996). Development and application of a two-site enzyme immunoassay for the determination of ‘total’ activin-A concentrations in serum and follicular fluid. J. Endocrinol. 148, 267-279.
Kruip T.A.M. and Dieleman S.J. (1982). Macroscopic classification of bovine follicles and its validation by micromorphological and steroid biochemical procedures. Reprod. Nutr. Dev. 22, 465-473.
Larocca C., Kmaid S. and Calvo J. (1993). Effect of follicular fluid and estrous cow serum on maturation, fertilization and development of the bovine oocyte in vitro. Theriogenology. 39. 253 (Abstr.).
Leibfried-Rutledge M.L., Critser E.S., Eyestone W.H., Northey D.L. and First N.L. (1987). Development potential of bovine oocytes matured in vitro or in vivo. Biol. Reprod. 36, 376-383.
Lobo R.A., Di Zerega G.S. and Marrs R.P. (1985). Follicular fluid steroid levels in dysmature and mature follicles from spontaneous and hyperstimulated cycles in normal and anovulatory women. J. Clin. Endocrinol. Metab. 60, 81-87.
Malik R.K., Lohan I.S., Dhanda O.P., Hoodaand O.K. and Singh S. (1999). Peritoneal fluid from rabbits or goats as media for in vitro maturation, fertilization and initial culture of caprine oocytes. Anim. Reprod. Sci. 54, 195-201.
Martino A., Mogas T., Palomo M.J. and Paramino M.T. (1995). In vitro maturation and fertilization of prepubertal goat oocytes. Theriogenology. 43, 473-485.
Mondal A., Khandoker M.A.M.Y., Mondal M.A., Rahman A.H.M.S., Apu A.S. and Pervage S. (2008). In vitro production of goat embryos. Bang. J. Anim. Sci. 37, 1-9.
Morgan P.M., Boatman D.E. and Bavister B.D. (1990). Relationships between follicular fluid steroid hormone concentrations, oocyte maturity, in vitro fertilization and embryonic development in the rhesus monkey. Mol. Reprod. Dev. 27, 145-151.
Naito K., Fukuda Y. and IshibashiI.(1989). Developmental ability of porcine ova matured in porcine follicular fluid in vitro and fertilized in vitro. Theriogenology. 31, 1049-1057.
Nandi S., Raghu H.M., Ravindranatha B.M., Gupta P.S.P. and Sarma P.V. (2004). In vitro development of buffalo oocytes in media-containing fluids from different size class follicles. Reprod. Domest. Anim. 39, 33-38.
Rahman A.N.M.A., Abdullah R.B. and Wan-Khadijah W.E. (2008).In vitro maturation of oocytes with special reference to goat: A review. Biotechnology. 7, 599-611.
Rahman M.G.M., Goswami P.C., Khandoker M.A.M.Y., Tareq K.M.A. and Ali S.Z. (2004). Collection of bovine cumulus-oocyte complexes (COCs) from slaughterhouse ovaries in Bangladesh. Pak. J. Bio. Sci. 6, 2054-2057.
Rajikin M.H., Yusoff M. and Abdullah R.B. (1994). Ultrastructural studies of developing goat oocytes in vitro. Theriogenology. 42, 1003-1016.
RhoG.J., Hahnel A.C. and Betteridge K.J. (2001). Comparisons of oocyte maturation and of three methods of sperm preparation for their effects on the production of goat embryos in vitro. Theriogenology. 56, 503-516.
Rodriguez G.E., Lopez B.M., Velilla E. and Paramio M.T. (2001). A Blue Cresyl Brilliant (BCB) test and its maturation in the presence of cysteamine for the improvement of the efficiency of in vitro production of embryos from prepubertal goat oocytes. IX Jornadas sobre produccion animal, Zaragoza, Spain. ITEA. 22, 796-798.
SAS. Statistical Analysis System, Version 6.03. SAS Institute Inc. CaryNC, 1998; 25-109 USA.
Shabankareh K.H. and Sarsaifi K. (2008). Developement potential of ovine oocytes cultured in different maturation media, Effect of human monoposal serum. J. Biotec. 10, 10-16.
Suchanek E., Simunic V., Juretic D. and Grizelj V. (1994). Follicular fluid contents of hyaluronic acid, follicle-stimulating hormone and steroids relative to the success of in vitro fertilization of human oocytes. Fertil. Steril. 62, 347-352.
Tajik P. and EsfandabadiN.S.(2003). In vitro maturation of caprine oocytes in different culture media. Small Rumi. Res. 47, 155-158.
Wang Z.G., Xu Z.R. and YuS.D.(2007). Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat. Czech J. Anim. Sci. 52, 21-25.
Yoshida M., Ishigaki Y., Kawagishi H., Bamba K. and Kojima Y. (1992). Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizability and developmental capacity in vitro. J. Reprod. Fertil. 95, 481-488.