Microsatellite Analysis DNA for Controlled Breeding of Cervidae and Genetic Breeding of White Colored Cervus elaphus in Czech Republic

Document Type: Research Articles


1 Department of ForestProtection and Game Management FFWT, MendelUniversity, Brno, Czech Republic

2 Department of Animal Morphology, Physiology and Genetics FA, MendelUniversity, Brno, Czech Republic

3 Xcell SlovakiaBreeding Services, SlovakRepublic

4 Department of Hygiene and Food Safety, Faculty of Biotechnology and Food Science, SlovakUniversityof Agriculture, SlovakRepublic

5 Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy in Hradec Králové, Charles University, Prague, CzechRepublic


A territorial isolation is a danger to the game population, causing deterioration called the inbreeding depression. The typical signs are anomalous body proportions, such as short lower jaw, shortened lifetime, weak physique and lower birth rate. This is caused by low number of game in the community. White colored deer is in the location of the CzechRepublicbred in separation since 18th century. For their preservation, it was necessary to refresh the population by 'wild colored' Cervus elaphus. By this way, it was reduced the inbreeding depression and population was saved. Simultaneously white colored, beige colored, red colored, combined colored and spotted individuals started to appear. For further breeding development (retention of good health condition, fertility, inbreeding reduction and increase of white colored deer population), practical exploitation of molecular genetics methods were made by deer identification and verification of family relationships. Proven results were used for compilation of breeding groups with target of gradual reduction in: inbreeding, negative consequences of inbreeding depression and increase of white deer population (the origin from Kashmiror Persia). These breeding mechanisms are considered as breeding with controlled reproduction. This project is researching genetic variances within separated white deer population in game-preserve Žleby from 2004 to 2010. The idiotypes have been determined for thirteen microsatellite frequencies (BM888, OarFCB5, RM188, RT1, RT13, T26, T156, T193)and the genetic diversity, heterozygote contribution, polymorphic information content (PIC) and inbreeding factor (f) have been assessed during the project elaboration. Software Power Marker V 3.25 was used for elaboration of obtained results. The project results demonstrated the exploitation of molecular genetic methods for controlled game reproduction.


Belkhir K., Borsa P., Chichi L., Raufaste N. and Bonhomme F. (1996). Genetics 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, DNRS UMR 5171, Université de Montpellier II, Montpellier France. Available: http://www.univ-montp2.fr/~genetix/ genetix/genetix.htm.
Broders H.G., Mahoney S.P., Montevecchi W.A. and Davidson W.S. (1999). Population genetic structure and the effect of founder events on the genetic variability of moose, Alces alces, in Canada. Mol. Ecol. 8, 1309-1315.
Frantz A.C., Hamann J.L. and Klein F. (2008). Fine-scale genetic structure of red deer(Cervus elaphus) in a French temperate forest. Eur. J. Wildl. Res. 54, 44-52.
Hmwe S.S., Zachos F.E., SaleJ.B., Rose H.R. and Hartl G.B. (2006). Genetic variability and differentiation in red deer(Cervus elaphus) from Scotlandand England. J. Zoology. 270(3), 479-487.
Lenny Williams C., Sarfass T.L., Cogan R. and Rhodes O.W. (2002). Microsatellite variation in the reintroduced Pennsylvaniaelk her. Mol. Ecol. 11, 1299-1310.
Liu K. and Muse S.V. (2005). Power Marker: Integrated analysis environment for genetic marker data. Bioinformatics. 21, 2128-2129.
Pérez-Espona S., Pérez-Barbería F.J., McLeod J.E., Jiggins C.D., Gordon I.J. and Pemberton J.M. (2008). Landscape features affect gene flow of Scottich Highland red deer(Cervus elaphus). Mol. Ecol. 17(4), 981-996.
Slate J. and Pemberton J.M. (2007). Admixture and patterns of linkage disequilibrium in a free-living vertebrate population. J. Evol. Biol. 20(4), 1415-1427.
Tullová M. (2008). Genetická premenlivosť populácií jeleňa lesnného (Cervus Elaphus L.) na Slovensku a v Európe. Act. Fac.Forest. Zvolen. 50, 7-18.
Webley L.S., Zenger K.R., English A.W. and Cooper D.W. (2004). Low levels of genetic variation within introduced Javan rusa deer (Cervus timorensis russa) in Australia. Eur. J. Wildl. Res. 50, 137-140.
Webley L.S., Zenger K.R., Hall G.P. and Cooper D.W. (2007). Genetic structure of introduced European fallow deer (Dama dama dama) in Tasmania, Australia. Eur. J. Wildl. Res. 53, 40-46.
Zachos F.E., Althoff C., Von Steynitz Y., Eckert I.and Hartl G.B. (2007). Genetic analysis of an isolated red deer(Cervus elaphus) population showing signs of inbreeding depression. Eur. J. Wildl. Res. 53, 61-67.