Effect of Thyroid Activity Modulation on Some Histological and Biochemical Aspects in Broiler Chicks


1 Department of Poultry Production, Faculty of Agriculture, Ain Shams University, Shobra El-Kheima, Cairo, Egypt

2 Department of Animal Production, National Research Centre, Dokki, Cairo, Egypt


An experiment was conducted to investigate the effect of thyroid gland activity modulation (Hyper or hypothyroidism) on energy utilization in broiler chickens. Two hundred and forty one-day-old Cobb broiler chicks were distributed into three dietary metabolizable energy (ME) treatment groups (80 chicks each). The control group (E0) was fed basal diet (3150 kcal/kg diet), the second group (E1) was fed low ME diet (3000, kcal/kg diet) with different thyroidal treatments and the third group (E2) was fed very low ME diet (2850 kcal/kg diet) with thyroidal treatments. Thyroidal treatments were applied at the beginning of the 2nd week, where (T0) was a control treatment; two hyperthyroidism groups induced by administration of Eltroxin (T1) or calcium iodide (T2), and hypothyroidism group induced by carbimazole administration (T3). Results showed that plasma thyroidal hormones (T3, T4) concentrations and their ratio T3 / T4 showed considerable changes related to thyroidal treatments. Plasma glucagon (G) level was significantly increased while insulin (I) level and I / G ratio were significantly decreased as affected by lowenergy diets. Carbimazole administration group had the lowest plasma insulin level and I / G ratio compared to other treatments. Moreover, calcium iodide significantly increased adenosine triphosphate (ATP), Total adenylate(TA) and phosphatepotential(PP) while carbimazole significantly decreased adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Histological examination of thyroid gland sections reflect the beneficial use of calcium iodide (CaI) as a safe additive without hazards effect on thyroid gland histology. Results suggested that calcium iodide could be used to maximize the utilization of lowenergy diets, via its modulating action of thyroid gland activity.


Abdelatif A.N. and Elkhair M.N. (2009). Effect of seasonal change in the thermal environment on physiological responses of unsexed broilers to dietary supplementation of antithyroid drug carbimazole. Middle-East J. Scientific Res. 4(2), 122-126.
Akhlaghi A. and Zamiri M.J. (2007). Effect of transient prepubertal hypothyroidism on serum testosterone level and seminal characteristics of chickens.Iranian J. Vet. Res. 8(1), 23-31.
Akiba Y., Jensen L.S., Bart C.R. and Kraeling R.R. (1982). Plasma estradiol, thyroid hormones and liver lipids determination in birds. J. Nutr. 112, 299-308.
Atkinson D.E. and Walton A.W. (1974). The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifier. Biochem. (7), 4030-4034.
Blem C.R. (1990). Avian energy storage. Pp. 59-113 in Current Ornithology. D.M. Power, Ed. vol. 7. Plenum, New york.
Buyse J., Decuypere E. and Simon J. (1990). The effect of thyroid hormone status on plasma glucose-insulin interrelationship in broiler chickens. Reprod. Nutr. Dev. 30(6), 683-692.
Chopra G.N., Chua-Teco J.B., Eisenberg W.M. and Solomon D.H. (1982). Structure-activity relationship of inhibition of hepatic monodeiodination of thyroxine to 3,5,3-triodothyronine by thiouracil and related compounds. Endocrinology. 110, 163-168.
Colca J.R. and Hazelwood R.L. (1982). Persistence of immunoreactive insulin, glucagon and pancreatic polypeptide of plasma in depancreatized chickens. J. Endocrinol. 92, 317-326.
Danforth E.J. and Burger A. (1984). The role of thyroid hormones in the control of energy expenditure. Clin. Endocrinol. Metab. 13, 581-595.
Duncan D.B. (1955). Multiple range and multiple F tests. Biometrics. 11, 1- 42.
Gilman A.G. and Murad F. (1975). Thyroid and antithyroid drugs. Pp. 1398-1422 in Pharmacological Basis of Therapeutics. Goodman, L.S. and A.G. Gilman Ed. Macmillan. New York.
Hazelwood R.L. (1989). Somatostatin: Regulator of I / G molar ratios. Pp. 144-146 in The Endocrine Pancreas, A.M. Gladsa, Ed. Prentice- Hall, New Jersey.
Hazelwood R.L. (1993). The pancreatic polypeptide family: vascular, gastrointestinal, and feeding behavioral implications. Proc. Soc. Exp. Biol. Med. 202, 44-63.
Hazelwood R.L. (2000). Pancreas. Pp. 539-555 in Sturkie’s Avian Physiology, G Causey Whittow. Ed. 5th Ed. Academic Press. CA.
Henry R.J. (1974). Clinical Chemistry: Principles and Techniques. NY: Harper and Row. New York.
Jaworek D., Gruber W. and Bermeyer H.U. (1974). Adenosine- 5- diphosphate and adenosine- 5- monophosphate. Pp. 432-533 in Methods of Enzymatic Analysis. H.U. Bergmeyer, Ed. Verlage Chemie Weinheim, Academic Press, London.
Lamprecht W. and Trautschold I. (1974). Determination of adenosine- 5- triphosphate with hexokinase and glucose- 6- phosphate dehydrogenase. Pp. 432-533 in Methods of Enzymatic Analysis. H.U. Bergmeyer, Ed. Verlage Chemie Weinheim, Academic Press, London.
Liu J.S., Chen Y.Q. and Li M. (2006). Thyroid hormones increase liver and muscle thermogenic capacity in the little buntings (Emberiza pusilla). J. Thermal. Biol. 31, 386-393.
Martin D.W. (1976). Nucleotides. Pp. 122-131 in Review of physiological chemistry. H.A. Harper. and Associate Authors. Ed. 16th Ed., Los Altos, California.
McNabb F.M.A. (2000). Thyroids. Pp. 461-471 in Sturkie’s Avian Physiology. GC Whittow. Ed. San Diego: Academic Press.
Moravej H., Khazali H., Shivazad M. and Yeganeh H.M. (2006). Plasma concentrations of thyroid hormone and growth hormone in Lohmann male broilers fed on different dietary energy and protein levels. Int. J. Poult. Sci. 5(5), 457-462.
Rosebrough R.W. and McMurtry J.P. (2003). Methimzole and thyroid hormone replacement in broilers. Domest. Anim. Endocrinol. 24, 231-242.
Rosebrough R.W., McMurtry J.P., Mitchell A.D. and SteeleN.C. (1988). Chicken hepatic metabolism in vitro. Protein and energy relations in the broiler chicken. -6. Effect of dietary protein and energy restrictions on in vitro carbohydrate and lipid metabolism and metabolic hormone profiles. Comp. Biochem. and Physiol. B-Comp. Biochem. 90(2), 311-316.
SAS (1994). SAS/STAT®User's Guide: Statistics Ver. 6.04, 4th ed. SAS Institute Inc. Cary, NC.
Shimizu T., Sasakuma F. and Ishikawa O. (1994). Assessment of immunoassay for insulin in diagnostic tests. Diab. Res. Clin. Pract. 26(2), 149-152.
Tur J.A., Rial R.V., Tur J. and Planas B. (1987). Effect of thyroidal state on the gastrointestinal transit and emptying of young broilers.Comp. Biochem. Physiol. 87(3), 665-670.
Volume 2, Issue 4 - Serial Number 4
December 2012
Pages 367-375
  • Receive Date: 23 May 2011
  • Revise Date: 22 June 2011
  • Accept Date: 15 July 2011
  • First Publish Date: 01 December 2012