Genotypic and Allelic Frequencies of IGF1 and IGF2 Genes in Broilers Analysed by Using PCR‐RFLP Techniques


1 Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India

2 Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India

3 Department of Genetics, ARIBAS, New V.V. Nagar, Anand, India


A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 butA-allele (0.5611) IGF2 in the broiler population.


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Volume 2, Issue 4 - Serial Number 4
December 2012
Pages 357-360
  • Receive Date: 08 September 2011
  • Revise Date: 29 October 2011
  • Accept Date: 30 October 2011
  • First Publish Date: 01 December 2012