1Biomin Holding GmbH, Industriestrasse 21, 3130, Herzogenburg, Austria
2Department of Animal Nutrition and Feeds, Institute of Animal Science of Lithuanian Veterinary Academy, R. Žebenkos 12, 82317, Baisogala, Lithuania
Receive Date: 30 November 2011,
Revise Date: 27 December 2011,
Accept Date: 28 December 2011
A silage inoculant [Biomin® BioStabil Plus, BSP, a blend of Enterococcus faecium (DSM 3530), Lactobacillus brevis (DSM 19456) and Lactobacillus plantarum (DSM 19457)], was used on legume-grass silage [32% of dry matter (DM)] vs. an untreated control silage (CT). The material had mean crude protein (CP) and water soluble carbohydrate (WSC) concentrations of 174 and 88 g/kg,respectively. BSP resulted in significantly higher CP (159 vs. 149 g/kg DM; P<0.05) and digestible protein concentrations (117.8 vs. 108.9 g/kg DM; P<0.01). Inoculant increased fermentation rates, resulting in a significant decrease in pH (P<0.05) and a significant increase of total fermentation acids concentration (P<0.05), as well as higher quantity of lactic acid (P<0.01) and higher content of acetic acid compared to CT. Butyric acid and Ammonia N concentrations were significantly decreased (P<0.01) through the use of BSP. Dry matter (DM) losses were significantly lower (P<0.01) usingBSP treated grass-legume silages. The digestible energy (P<0.01) and net energy lactation (P<0.05) were higher in the inoculated silage compared to the CT (2.1 and 1.25%, respectively). The inoculation of silage with BSP also improved stability. Twenty-four dairy cows were fed with both type of silages and their productivity response was evaluated over a 92-days feeding period. Animals were assigned to two treatments in a randomized-block design experiment. Silage was offered ad libitum to animals in both treatments and cows were supplemented with 4 kg DM of a commercial compound feed per day. The silage DM feed intake was higher in BSP (+6.5%), whereas the energy corrected milk (ECM) production for treated silages increased by 1.4 kg per cow per day. The milk fat and protein content were numerically higher in animals under the treatment with BSP and the efficiency of the conversion of feed-NEL into milk was significantly higher (P<0.05) in cows which were fed with the silage treated using BSP.
DannerH., Holzer M., Mayrhuber E. and Braun R. (2003). Acetic acid increases stability of silage under aerobic conditions. App. Environ. Microb. 69(1), 562-567. Filya I., Ashbell G., Hen Y. and Weinberg Z.G. (2000). The effect of bacterial inoculants on the fermentation and aerobic stability of whole crop wheat silage. Anim. Feed Sci. Technol.88, 39-46. Inglis G.D., Yanke L.J., Kawchuk L.M. and Mc Allister T.A. (1999). The influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. Canadian J. Microbiol. / Rev. Canadian Microbiol. 45(1), 77-87. Jatkauskas J. and Vrotniakiene V. (2006). Effect of Lactobacillus rhamnosus andPropionibacterium freudenreichii inoculated silage on nutrient utilization by dairy cows. Veterinarija ir Zootechnika. 36(58), 17-20. Kramer W. (2002). Neue Entwicklungen und Strategien im Bereich der Silierzusätze. 8. Alpenländisches Expertenforum, 9-10 April, Bundsanstalt für alpenländische Landwirtschaft, Österreich. Kung L.Jr. (2000). Use of forage additives in silage fermentation. 2000-2001. Pp. 39-44in Direct-fed Microbial, Enzyme and Forage Additive Compendium.The Miller Publishing Company, Minnesota, USA. Kung L.Jr. and Muck R.E. (1997). Animal responses to silage additives. Pp. 200-210in Proc. From the Silage: Field to Feedbunk North American Conference, Hershey, Pennsylvania, USA. Merry R.J., Jones R. and Theodorou M.K. (2000). The conservation of grass. Pp. 196-228 in Grass, Its Production and Utilisation, Hopkins A. (ed.), 3rd Ed., Oxford: UK, Blackwel Science Ltd. Site of Publication. Muck R.E. and Kung L.Jr. (1997). Effects of silage additives on ensiling. Pp. 187-199 in Silage: Field to Feedbunk. NRAES-99. Northeast Reg. Agric. Eng. Serv., Ithaca, New Yprk. Ohmomo S., Tanaka O., Kitamoto H.K. and Cai Y. (2002). Silage and microbial performance, old story but new problems. Jap. Agric. Res. 36(2), 59-71. Pahlow G., Rammer C., Slottner D. and Tuori M. (2002). Ensiling of legumes. Landbauforschung Voelkenrode Sonderheft. 234, 27-32. Pennington J. (2011). Reducing somatic cell count in dairy cattle. Cooperative Extension Service, Division of Agriculture, Uni-versity of Arkansas, FSA 4002. http://www.uaex.edu/Other_Areas/publications/PDF/FSA4002.pdf Accessed 01.11.2011. Muck R.E., Filya I. and Contreras-Govea F.E. (2007). Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production. J. Dairy Sci.90, 5115-5125. Ranjit N.K. and Kung L.Jr. (2000). The effect of Lactobacillus buchneri, Lactobacillus plantarum, or chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci.83, 526-535. Schukken Y.H., Wilson D.J., Welcome F., Garrison-Tikofsky L. and Gonzalez R.N. (2003). Monitoring udder health and milk quality using somatic cell counts. Vet. Res. 34, 579-596. Weddell J.R., Agnew R. and Cottrill B. (2002). The UK Forage Additives Approval Scheme - Developments and Products Approvals. Pp. 230-231 in Proc. 8th International Silage Conference. Auchincruive, Scotland. Weinberg Z.G., Ashbell G., Hen Y., Azrieli A., Szakacs G. and Filya I. (2002). Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri. J. Ind. Microbiol. Biotechnol.28, 7-11. Weinberg Z.G., Muck R.E., Weimer P.J., Chen Y. and Gamburg M. (2004). Lactic acid bacteria used in inoculants for silage as probiotics for ruminants. App. Biochem. Biotechnol.118, 202-209. Wilkins R.J., Syrjala-Qvist L. and Bolsen K.K. (1999). The future role of silage in sustainable animal production. Pp. 23-40 inProc. 7th International Silage Conference. Uppsala, Sweden. Winters A.L., Fychan R. and Jones R. (2001). Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance. Grass.Forage.Sci. 56, 181-192. Winters A.L., Lloyd J., Leemans D., Lowes K. and Merry R. (2002). Effect of inoculation withLactobacillus plantarum on protein degradation during ensilage of red clover.Pp. 108-109 in Proc. 8th International Silage Conference,Auchincruive, Scotland. Ziggers D. (2003). Good or bad guys determine silage quality. Dairy beef.2, 27-29.