Sperm Transport in the Mare Reproductive Tract: A Review

Document Type: Review Articles

Author

Department of Morphology, Biology Institute, Federal University of Pelotas, Pelotas, RS, Brazil

Abstract

The objective of this study was to review the literature on sperm transport in the mare. During this process the sperm cells must reach the oviducts in order to fertilize the oocyte, but before reaching this site it must go through the uterus that is a hostile environment at this moment since there is an inflammatory reaction in this location that aims to eliminate excess spermatozoa and bacterial contaminants. Sperm cells can be observed 30 minutes after artificial insemination and remain in the uterine tubes for at least 24 hours. It was found that the sperm cells can be observed in 62.6% of the mares after insemination when using light microscopy, both in the uterine glands and the epithelium of the uterus. Uterine glands may act as a reservoir for sperm. After artificial insemination the number of mares with spermatozoa in the uterine epithelium, glands and utero-tubal junction decreases over time.

Keywords


Alghamdi A., Troedsson M.H.T., Laschkwitsch T. and Xue J.L. (2000). Uterine secretion from mares with post-breeding endometritis alters motion characteristics in vitro. Theriogenology.55, 1019-1028.
Allen W.R., Bowen J.M., Frank C.J., Jeffcott L.B. and Rossdale P.D. (1976). The current position of AI in horse breeding. Equine Vet. J. 8, 72-74.
Bader H. (1982). An investigation of sperm migration into the oviducts of the mare. J. Reprod. Fertil. 32, 59-64.
Bader H. and Krause A. (1982). Investigations about the transport, distribution and the fate of the spermatozoa in the genital tract of the mare. Proc. Int. Cong. Anim. Reprod. 5, 197-205.
BedfordS.J. and Hinrichs K. (1994). The effect of insemination volume on pregnancy rates of pony mares. Theriogenology. 42, 571-578.
Brinsko S.P., Varner D.D., Blanchard T.L. and Meyers S.A. (1990). The effect of post breeding uterine lavage on pregnancy rate in mares. Theriogenology. 33, 465-475.
Brinsko S.P., Varner D.D. and Blanchard T.L. (1991). The effect of uterine lavage performed four hours post insemination on pregnancy rates in mares. Theriogenology. 35, 1111-1119.
Demick D.S., Voss J.L. and Picket B.W. (1976). Effect of cooling, storage glycerolization and spermatozoal numbers on equine fertility. J. Anim. Sci. 43, 633-637.
Ebertus R. (1963). The dilution of stallion semen with whole cow milk. Anim. Breed. 31, 313-318.
Fiala S.M., Jobim M.I.M., Gregory R.M. and Mattos R.C. (2007a). Sperm transport in the oviduct and uterus after AI indifferent times. Pferdeheilkunde, Aceito Para Publicação.
Fiala S.M., Pimentel C.A., Mattos A.L.G., Gregory R.M. and Mattos R.C. (2007b). Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare. Theriogenology. 67, 556-562.
Fiala S., Cruz L.A., Rodrigues R., Jobim M.I., Gregory R.M. and Mattos R.C. (2010). Sperm cells in the reproductive tract of the mare: Where can we find them Pferdeheilkunde. 26, 19-21.
Hunter R.H.F. (1981). Sperm transport and reservoirs in the pig oviduct in relation to the time of ovulation J. Reprod. Fertil. 63,109-117.
Jasko D.J., Moran D.M., Farlin M.E. and Squires E.L. (1991). Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen. Theriogenology. 36, 1059-1067.
Jasko D.J., Hathaway J.A., Schaltnbrand V.L., Simper W.D. and Squires E.L. (1992). Effect of seminal plasma end egg yolk on motion characteristics of cooled stallion semen. Theriogenology. 37, 1241-1252.
Jones D. (1995). Fluid distribution and cervical loss following intrauterine infusion in the mare. Eq. Pract. 17, 12-19.
Katila T. (1995). Onset and duration of uterine inflammation response of mares with fresh semen. Biol. Reprod. Mono. 1, 515-517.
Katila T. (1997). Interactions of the uterus and semen. Pferdeheilkunde. 13, 508-511.
Katila T., Sankari S. and Mäkelä O. (2000). Transport of spermatozoa in the reproductive tracts of mares. J. Reprod. Fertil. Suppl. 56, 571-578.
Keller A., Malschitzky E., Hött A., Vieira M.J., Gregory R.M. and Mattos R.C. (2001). Effect of method of seminal plasma removal, extender and length of storage on motility and fertility of equine semen. Anim. Repr. Sci.318-319.
Kneissl S. (1993). Tiefgefrierkonservierung von Pferdesperma: Einfluss der Samen- entnahmetechnik, Zentrifugation, Konfetionerungsform und Einfriermethode auf die Motilität und Membranintegritaet der Samenzellen. Tese (Doutorado em Medicina Veterinária) Hannover, Tierärztl. Hochsch.
Kotilainen T., Huhtinen M. and Katila T. (1994). Sperm-induced leucocytosis in the equine uterus. Theriogenology.41, 629-636.
Leblanc M.M., Neuwirthl L. and Asbury A.C. (1994). Scintigraphic measurement of uterine clearance in normal mares and mares with recurrent endometritis. Equine Vet. J. 26, 109-113.
Mann T., Polge C. and Rowson L.E.A. (1956). Participation of seminal plasma during the passage of spermatozoa in the female reproductive tract of the pig and horse. J. Endocr. 13, 133-140.
Marden W. and Werthessen N.T. (1956). Influence of seminal fluid on sperm motility. Fertil. Steril. 7, 508-515.
Merkt H., Bader H. and Klug E. (1982). Die bedeutung klinisch andrologischer untersunchungen bei Hengsten für deren praktischen Zuchteinsatz. Dtsch. Tierarztl. Wschr. 89, 219-223.
Padilla A.W. and Foote R.H. (1991). Extender and centrifugation effects on the motility patterns of slow-cooled stallion spermatozoa. J. Anim. Sci.69, 3308-3313.
Pickett B.W. and Back D.G. (1973). Procedures for preparation, collection evaluation and insemination of stallion semen. ColoradoState UniversityExp. Sta. Anim. Reprod. Lab. Gen. Series. Pp. 935.
Pickett B.W., Back D.G., Burwash L.D. and Voss J.L. (1974). The effect of extenders, spermatozoal numbers and rectal palpation on equine fertility. Proc. NAAB Tech. Conf. Artif. Insem. Reprod. 5, 47-58.
Pickett B.W., Sullivan J.J., Byers W.W., Pace M.M. and Remmenga E.E. (1975). Effect of centrifugation and seminal plasma on motility and fertility of stallion and bull spermato zoa. Am. Fert. Soc.26, 743-746.
Pickett B.W. and Amann R.P. (1987). Extension and storage of stallion spermatozoa: A review. J. Equine Vet. Sci.7, 289-302.
Rowley M.S., Squires E.L. and Pickett B.W. (1990). Effect of insemination on embryo recovery in mares. Equine. Vet. Sci. 10,298-300.
Scott M.A., Liu I.K.M., Robertson K.R., Hanrath M., Overstrett J.W. and Drobnis E.Z. (1994). Acrossomal status and movement characteristics of sperm in the oviducts of normal mares. Pp. 173-174 inProc. Int. Symposium Eq. Reprod., Caxambu, Brasil,
Scott M.A., Liu I.K.M. and Overstreet J.W. (1995). Sperm transport to the oviducts: abnormalities and their clinical implications. Proc. Ann Conv. Am. Assoc. Eq. Pract. 41, 1-2.
Scott M.A. (2000). A glimpse at aperm function in vivo: Sperm transport and epithelial interaction in the female reproductive tract. Anim. Reprod. Sci. 60-61, 337-348.
Sieme H., Schäfer T., Stout T.A.E., Klug E. and Waberski D. (2003). The effects of diferent insemination regimes on fertility in mares. Theriogenology. 60, 1153-1164.
Squires E.L., Amann R.P., Mckinnon A.O. and Pickett B.W. (1998). Fertility of equine spermatozoa cooled to 5 or 20 ˚C. In:Haia. Proc. Inter. Cong. Anim. Reprod. Art. Insem. 3, 297-299.
Thomas P.G.A., Ball B.A. and Brinsko S.P. (1994). Interaction of equine spermatozoa with oviduct epithelial cell explants is affected by estrous cycle and anatomic origin of explant. Biol. Reprod. 51, 221-228.
Troedsson M.H.T. (1995). Uterine response to semen deposition in the mare. Pp. 130-134 inProc. For Ann. Meet. Soc. Theriog.
Troedsson M.H.T., Steiger B.N, Ibraihm N.M., Foster D.N. and Crabo B.G. (1995a). Mechanism of sperm-induced endometritis in the mare. Biol. Reprod. Suppl. 52, 307.
Troedsson M.H.T., Crabo B.G, Ibraihm, N.M., Scott M. and Ing M. (1995b). Mating induced endometritis: Mechanisms, clinical importance, and consequences. Proc. Am. Assoc. Eq. Pract. 41, 11-12.
Troedsson M.H.T. (1997). Therapeutic considerations for mating induced endometritis. Pferdeheilkunde. 13, 516-520.
Troedsson M.H.T., Liu I.K.M. and Crabo B.G. (1998). Sperm transport and survival in the mare. Theriogenology. 49, 905-915.
Troedsson M.H.T., Franklin R.K. and Crabo B.G. (1999). Suppression of PMN-chemotaxis by different molecular weight fractions of equine seminal plasma. Pferdeheilkunde. 15, 568-573.
Varner D.D., Blanchard T.L., Love C.L., Garcia M.C. and Kenney R.M. (1987). Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Theriogenology. 28, 709-723.
Watson E.D. and Nikolakopoulos E. (1996). Sperm longevity in the mare’s uterus. J. Equine Vet. Sci.16, 390-392.