Immune Response and Productive Performance of Dairy Buffaloes and their Offspring Supplemented with Black Seed Oil


Department of Animal Production, Facultyof Agriculture, AinShamsUniversity, Cairo, Egypt


Twenty pregnant buffaloes, eight weeks before the expected calving date, were assigned into two homogeneous groups of 10 animals each, according to their season of lactation (2nd to 8th season of lactation); The first group (G1) were fed on a ration consisting of concentrate feed mixture (22% yellow corn, 35% cotton seed meal, 33% wheat bran, 4% rice bran, 3% molasses, 2% limestone and 1% sodium chloride), rice straw and berseem (in winter) berseem hay or corn silage (in summer) according to the feeding regime of the traditional farm system. The second group (G2) was fed the control ration plus 10 ml black seed oil (BSO)/head/day. After calving, the offspring of buffaloes of each group were divided into two similar groups of five animals each, according to their birth weight. Calves of T1 and T2 represented the offspring of G1 buffaloes (the control group) while those of T3 and T4 belonged to buffaloes of G2. The results showed that nutrients digestibility were nearly similar for both G1 and G2 (P>0.05). Total plasma protein, albumin, GPT, GOT, plasma immunoglobulin and blood hematocrit for lactating buffaloes tended to be higher (P<0.05) in G2 than those of G1. Four days after parturition showed insignificant differences regarding total solid, fat, solid not fat, total protein, lactose and ash. The treated group (G2) showed higher (P<0.05) total solid, fat, solid not fat, total protein and ash content than those of G1, while lactose value was nearly similar. The results of buffalo calves showed that the highest (P<0.05) values of  dry matter, organic matter, crude protein, crude fiber, nitrogen free extract (except that of ether extract; P>0.05) digestibility were recorded for T2 compared to those of the other groups (T1, T3 and T4), but those in T3 showed the lowest values. Calves fed supplemented ration with BSO (T2 and T4) had higher nutrient digestibility values than those of the non supplemented groups (T1 and T3). No significant differences (P>0.05) were observed in plasma total protein, albumin, urea, creatin, total lipids, glucose, glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, triglyceride, hematocrite and neutrophil to lymphocyte ratio (NLR). On the contrary, plasma cholesterol concentration was decreased in treated groups (T2 and T4), while plasma immunoglobulin concentration was increased, regarding to calves, performance during the suckling period.


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Volume 1, Issue 4 - Serial Number 4
December 2011
Pages 227-234
  • Receive Date: 20 February 2011
  • Revise Date: 17 March 2011
  • Accept Date: 02 May 2011
  • First Publish Date: 01 December 2011