Survey of FecXL Locus of BMP15 Gene and Growth Hormone (GH) Gene and Their Effects on Lambing Rate in Zel Sheep

Document Type: Research Articles

Authors

Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

Abstract

The GH gene and BMP15 gene have been used as candidate genes for ­marker-assisted selection in different livestock species. Random blood samples were obtained from 180 Zel sheep breed to study genetic polymorphism of these genes. DNA was extracted from blood samples and a 365 bp fragment from the exon V of the ovine growth hormone gene and a 312 bp region from exon II of BMP15 gene were amplified by polymerase chain reaction. SSCP analysis showed three conformational patterns (A, B and C) for GH gene but for FecXL locus of BMP15 no banding patterns were observed in the animals tested. Results indicated that there were no significant associations (P>0.05) between polymorphism of the GH loci and lambing rate.

Keywords


Aaltonen J., Laitinen M.P., Vuojolainen K., Jaatinen R., Horelli-Kuitunen N., Seppa L., Louhio H., Tuuri T., Sjoberg J., Butzow R., Hovatta O., Dale L. and Ritvos O. (­1999). Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. J. Clin. Endocrinol. Metabolism. 84, 2744-2750.

Ahani Azari M., Yousefi S. and Dehnavi E. (2011). Evaluation of κ-casein and growth hormone genes polymorphism in native Dalagh sheep. Slovak J. Anim. Sci. 44(4), 129-133.

Baldi A. (1999). Manipulation of milk production and quality by use of somatotropin in dairy ruminants other than cow. Domest. Anim. Endocrinol. 17, 131-137.

Barracosa H. 1996. Estudo de polimorfismos genetic osedasuaassociação com capacidades de produç ãoleiteiraem caprinos de raçaAlgarvia e ovinos de raça Serra da Estrela. Dissertação de Mestrado, Univ. do Algarve, Faro, Portugal.

Bastos E., Cravador A., Azevedo J. and Guedes-Pinto H. (­2001). Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed “Churra da Terra Quente”. Biotechnol. Agron. Soc. Environ. 5, 7-15.

Bauman D.E. (1999). Somatotropin mechanism in lactating cows: from basic science to commercial application. Domest. Anim. Endocrinol. 17, 101-116.

Bodin L., Di Pasquale E., Fabre S., Bontoux M., Monget P., Ersani L. and Mulsant P. (2007). A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. J. Endocrinol. 148, 393-400.

Bodin L., San Cristobal M., Lecerf F., Mulsant P., Bibe B., Lajous D., Belloc J.P., Eychenne F., Amigues Y. and Elsen J.M. (2002). Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep. J. Genet. Selec. Evol. 34, 447-464.

Breier B.H. (1999). Regulation of protein and energy metabolismby the somatotropicaxis. Domest. Anim. Endocrinol. 17, 209-218.

Dube J.L., Wang P., Elvin J., Lyons K.M., Celeste A.J. and Matzuk M.M. (1998). The bone morphogenetic protein 15 gene is X-linked and expressed in oocytes. Mol. Endocrinol. 12, 1809-1817.

Folch J., Ramón J.P., Cocero M.J., Alabart J.L. and Beckers J.F. (2001). Exogenous growth hormone improves the number of transferable embryos in superovulated ewes. Theriogenology. 55(9), 1777-1785.

Galloway S.M., McNatty K.P., Cambridge L.M., Laitinen, M.P.E.J., Juengel L.T., Jokiranta S.R., McLaren J., Luiro K., Dodds K.G., Montgomery W., Beattie A.E., Davis G.H. and Ritvos O. (2000). Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage- sensitive manner. J. Nat. Genet. 25, 279-283.

Gluckman P.D., Mueller P.L., Kaplan S.L., Rudolph A.M. and Grumbach M.M. (1979). Hormone ontogeny in the ovine fetus.I. Circulating growth hormone in mid and late gestation. Endocrinology. 104, 162-168.

Gootwine E., Ofir R. and Yossefi S. (1996). Characterization of PvuII polymorphisms between the ovine growth hormone GH2-N and GH2-Z gene copies. Anim. Biotechnol. 7, 135-143.

Gootwine E., Suttie J.M., McEwan J.C., Veenvliet B.A., Littlejohn R.P., Fennessy P.F. and Montgomery G.W. (1998). The physiological effects of natural variation in growth hormone gene copy number in ram lambs. Domest. Anim. Endocrinol. 14, 381-390.

Hanrahan J.P., Gregan S.M., Mulsant P., Mullen M., Davis G.H., Powell R. and Galloway S.M. (2004). Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovisaries). J. Biol. Reprod. 70, 900-909.

Hazout A., Junca A.M., Ménézo Y., Demouzon J. and Cohen-Bacrie P. (2009). Effect of growth hormone on oocyte competence in patients with multiple IVF failures. Reprod. Biomed. Online. 18(5), 664-670.

Jaatinen R., Bondestam J., Raivio T., Hilden K., Dunkel L., Groome N. and Ritvos O. (2002). Activation of the bone morphogenetic protein signaling pathway induces inhibinbeta (B)-subunit mRNA and secreted inhibin B levels in cultured human granulosalutealcells. J. Clin. Endocrinol. Metab. 87, 1254-1261.

Koch J.M., Wilmoth T.A. and Wilson M.E. (2010). Periconceptional growth hormone treatment alters fetal growth and development in lambs. J. Anim. Sci. 88, 1619-1625.

Laitinen M., Vuojolainen K., Jaatinen R., Ketola I., Aaltonen J., Lehtonen E., Heikinheimo M. and Ritvos O. (1998). A novel growth differentiation factor-9 (GDF-9) 19 related factor is co-expressed with GDF-9 in mouse oocytes during folliculogenesis. Mech. Dev. 78, 135-140.

Marques M.R., Santos I.C., Belo C.C., Cravador A., (2001). Associations between SSCPs in the GH gene and milk traits in “Serra da Estrela” ewes. Pp. 57-82 in Proc. Int. Conf. Farm Anim. Endocrinol. BASE, Gembloux, Belgium.

Monteagudo L.V., Ponz R., Tejedor M.T., Lavina A. and SierraI. (2009). A 17 bp deletion in the bone morphogenetic protein 15 (BMP15) gene is associated to increased prolificacy. Anim. Reprod. Sci. 110, 139-146.

Nejati Javaremi A., Rahimi G. and Amiri S. (2007). Detection of polymorphism in FecXL gene assocaiated with twining in LouriBakhtiary sheep using PCR-SSCP. Pp. 55-63 in Prpc. 5th Nation. Biotecnol. Cong. Tehran. Iran.

Polly S., De S., Brahma B., Mukherjee A., Vinesh P.V., Batabyal S., Arora J.S., Pan S., Kumar Samanta A., Kumar Datta T. and Lal Goswami S. (2010). Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garolesheep. J. Trop. Anim. Health. Prod. 42, 985-993.

Saadat Noori M. and Siah Mansoor S. (1995). Sheep Husbandary and Management, 5th Ed. Afshari Publication, Tehran.

Santos I.C., Marques M.R., Belo C.C. and Cravador A. (2004). Polymorphism analysis at the growth hormone gene in Merino da Beira Baixa ewes. Biotechnol. Agron. Soc. Environ. 8, 40-41.

SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC.

Scaramuzzi R.J., Murray J.F., Downing J.A. and Campbell B.K. (1999). The effects of exogenous growth hormone on follicular steroid secretion and ovulation rate in sheep. Domest. Anim. Endocrinol. 17, 269-277.

Shiri S.A.K., Saghi D.A., Nasiri M.R., Emrani H., Montazer Torbati F. and Mohammadzade M. (2006). Survey of genetic diversity growth hormone and growth hormone receptor genes in Iranian indigenous sheep breed (kordian sheep) using a non-radioactive SSCP. Pp. 21-28 in Proc. 57th Ann. Meet. Europ. Asso. Anim. Prod. Antalya, Turkey.

Souza C.J.H., Mac Dougall C., Campbell B.K., Mc Neilly A.S. and Baird D.T. (2001). The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR-1B) gene. J. Endocrinol. 169, 1-6.

Tahmorespoor M., Ansary M., Taheri A. and Gholami H. (2011). PCR-SSCP variation of GH and STAT5A genes and their association with estimated breeding values of growth traits in Baluchi sheep. Anim. Biotechnol. 22, 37-43.

Veldhuis J.D., Anderson S.M., Shah N., Bray M., Vick T., Gentili A., Mulligan T., Johnson M.L., Weltman A., Evans W.S. and Iranmanesh A. (2001). Neuro physiological regulation and target-tissue impact of the pulsatile mode of growth hormone secretion in the human. Growth Horm. IGF Res. 11, 25-37.

Zare Y., Nejati Javaremi A. and Rahimi G. (2007). Detection of polymorphisms in two points of the gene assocaiated with twining (BMP15) in Shalsheep. Pp. 72-81 in Proc. 5th National Biotecnol. Cong. Iran. Thehran, Iran.