Comparative Study of Probiotic, Acidifier, Antibiotic Growth Promoters and Prebiotic on Activity of HumoralImmune and Performance Parameters of Broiler Chickens


1 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

2 Department of Animal Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

3 Department of Animal Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran


The aims of this study were the comparative study of probiotic, acidifier,antibiotic growth promoters and prebiotic on activity of humoral immune and performance parameters of broiler chickens.500 one day old male broilers (308 Ross strain) were divided into 5 groups: A, B, C, D and E. Each group with equal numbers of male included 4 replicates (25 chicks per replicate). Group A was as control and the other groups were administrated with distinct dose of drugs, comprised: Virginamycine 150 gr/ton, protexin 100 gr/ton, salkil 6 kg/ton, immunoval 1 kg/ton, respectively. 40 broilers were selected from each group randomly and performance parameters such as weight, mortality, amount of grain for consumption, feed conversion rate (FCR) were calculated by means of scale and reference formula. Moreover, at three time points (9th, 17th and 24th days) about 1 cc of blood was taken from broilers’ brachial vein, within each of the five groups. Laboratory analysis conducted on blood samples featured Hemagglutination Inhibition (HI) test performed on sera and humoral immunity assessed as antibody production to Newcastle disease virus. The results showed that the group C and the group E were the best groups in terms of the performance parameters and the HI rate.


Bailey J.S., Blankenship L.C. and Cox N.A. (1991). Effect of fructooligosaccharideson Salmonella colonization of the chicken intestine. Poult. Sci. 70, 2433-2438.
Bedford M. (2000). Removal of antibiotic growth promoters from poultry diets: implications and strategies to minimise subsequent problems. World 's Poult. Sci. J. 56, 347-365.
Bello F.D., Walter J., Hertel C. and Hammes W.P. (2001). In vitro study of prebiotic properties of levan-type exopolysaccharides from Lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis, Syst. Appl. Microbiol. 24, 232-237.
Chen Y.C., Nakthong C. and Chen T.C. (2005). Improvement of laying hen performance by dietary prebiotic chicory oligofructose and inulin. Int. J. Poult. Sci. 4, 170-178.
Cotter P.F., Malzone A., Paluch B., Lilburn M.S. and Sefton A.E. (2000). Modulation of humoral immunity in commercial laying hens by a dietary prebiotic. Poult. Sci. 79, 38-44.
Elwinger K., Berndtson E., Engstrom B., Fossum O. and Waldenstedt L. (1998). Effect of antibiotic growth promoters and anticoccidials on growth of Clostridium perfringens in the caeca and on performance of broiler chickens. Acta. Vet. Scand. 39, 433-441.
George B.A., Quarles C.L. and Fagerberg D.J. (1982). Virginiamycin effects on controlling necrotic enteritis infection in chickens. Poult. Sci. 61, 447-450.
Hofacre C.L., Beacorn T., Collett S. and Mathis G. (2003). Using competitive exclusion, mannan-oligosaccharide and other intestinal products to control necrotic enteritis. J. Appl. Poult. Res. 12, 60-64.
Lemieux F.M., Southern L.L. and Binder T.D. (2003). Effect of mannanoligosaccharides on growth performance of weanling pigs. J. Anim. Sci. 81, 2482-2487.
Lutful Kabir S.M. (2009). The role of probiotics in the poultry industry. Int. J. Mol. Sci. 10, 3531-3546.
Milner J.A. and Roberforid M. (1999). Nutritional properties of inulin and oligofructose. J. Nutr. 129, 395-502.
Macfarlance G.T. and Cummings J.H. (1999). Probiotics and Prebiotics: can regulating the activities of intestinal bacteria benefit health. Est. J. Med. 171, 187-191.
Panda A.K., Reddy M.R., Rama Rao S.V., Raju M.V.L.N. and Praharaj N.K. (2000). Growth, carcass characteristics, immunocompetence and response to Escherichia coli of broilers fed diets with various levels of probiotic. Arch. Für Geflügelkunde. 64, 152-156.
Patterson J.A. and Burkholder K.M. (2003). Application of prebiotics and probiotics in poultry production. Poult. Sci. 82, 627-631.
Roberfroid M.B. ( 1998). Prebiotics and synbiotics: concepts and nutritional properties. Br. J. Nutr. 80, 197-202.
Salminen S., Bouley C. and Boutron-ruoult M.C. (1998). Function food science and gostroinestinal physiology and function. Bry. J. Nutr. 80, 147-171.
Savage T.F., Cotter P.F. and Zakrzewska E.I. (1996). The effect of feeding mannan oligosaccharide on immunoglobulins, plasma IgG and bile IgA, of Wrolstad MW male turkes. Poult. Sci. 75, 143-148.
Vegad J.L. (2004). Prebiotics, probiotic, acidfires and antibiotic growth promotors. Pp. 339-342, 343-346 in Poultry Diseases a Guide for Farmers and Poultry Professional. Int. Book Dist. Co. USA.
Zoppi G. (1998). Probiotics, prebiotics, synbiotics and eubiotics. Pediatr. Med. Chir. 20, 13-16.
  • Receive Date: 19 June 2012
  • Revise Date: 05 August 2012
  • Accept Date: 27 September 2012
  • First Publish Date: 01 June 2013