Total Antioxidant Capacity and Malondialdehyde Level in Plasma of Broiler Chicks Fed Diet Containing Different Levels of Ginger (Zingiber officinale)

Document Type : Research Articles


1 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Agricultural, Medical and Industrial Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Karaj, Iran


This study was conducted to assess the effect of ginger supplementation on malondialdehyde (MDA) level, as an oxidative stress marker, and total antioxidant capacity (TAC) in broiler chicks. Two hundred male 1-day-old chicks were assigned randomly to four dietary treatments (basal diet as control group and basal diet containing 2.5, 5.0 and 7.5 g/kg ginger, respectively), with five replicates and ten birds per replicate. The levels of MDA and TAC were measured at days 21 and 42 of age. At day 21, mean MDA levels in chicks fed diet containing 2.5 g/kg ginger decreased numerically, and decreased significantly (P<0.05) in chicks fed diet supplemented with 5 and 7.5 g/kg ginger, compared with that of control diet. At day 42, plasma MDA levels in chicks fed diets containing ginger decreased as compared with that of the control group. There were no differences for plasma MDA level among chicks fed diet containing ginger. There were significant differences (P<0.05) among treatments for TAC level. At day 21, the highest mean of TAC was found in plasma of chicks fed diet containing 7.5 g/kg ginger, and the lowest one was for chicks in the control group. At this period there was no difference between TAC level of chicks fed 2.5 and 5.0 g/kg ginger. At day 42, the similar results were observed, except that ginger supplementation over 5.0 g/kg had no significant effect on TAC level. The results showed that ginger supplementation, at and over 5.0 g/kg, caused improvement in the plasma of broiler chicks, with a decrease in MDA and an increase of (TAC).


Afshari A.T., Shirpoor A., Farshid A., Saadatian R., Rasmi Y., Saboory E., Ilkhanizadeh B. and Zllameh A. (2007). The effect of ginger on diabetic nephropathy, plasma antioxidant capacity and lipid peroxidation in rats. Food Chem. 101, 148-153.
Afzal M., Al-Hadidi D., Menon M., Pesek J. and Dhami M.S. (2001). Ginger: an ethno medical, chemical and pharmacological review. Drug Metabol. Drug Interact. 18, 159-190.
Ali B.H., Blunden G., Tanira M.O. and Nemmar A. (2008). Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale roscoe): a review of recent research. Food Chem. Toxicol. 46, 409-420.
Badreldin, H.A., Gerald, B., Musbah, O. and Nemmar A. (2008) Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem. Toxicol. 46, 409-420.
Bayraktar H., Altan Ö., Açıkgöz Z., Baysal S.H. and Şeremet C. (2011). Effects of oxidized oil and vitamin E on performance and some blood traits of heat-stressed male broilers. South African J. Anim. Sci. 41, 321-329.
Benzie I.F. and Strain J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power: the FRAP assay. Anal. Biochem. 239, 70-76.
Chrubasik S., Pittler M.H. and Roufogalis B.D. (2005).A comprehensive review on the ginger effect and efficacy profiles. Phytomed. 12, 684-701.
Erdogan Z., Erdogan S., Aksu T. and Baytok E. (2005). The effects of dietary lead exposure and ascorbic acid on performance, lipid peroxidation status and biochemical parameters of broilers. Turk J. Vet. Anim. Sci. 29, 1053-1059.
Fuhrman B., Rosenblat M., Hayek T., Coleman R. and Aviram M. (2000). Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apo lipoprotein E-deficient mice. J. Nutr. 130, 1124-1131.
Kikuzaki H. and Nakatani N. (1996). Cyclic diarylheptanoids from rhizomes of Zingiber officinale. Phytochem. 43, 273-277.
Kota N., Krishna P. and Polasa K. (2008). Alterations in antioxidant status of rats following intake of ginger through diet. Food Chem. 106, 991-996.
Krishnakantha T.P. and Lokesh B.R. (1993). Scavenging of superoxide anions by spice principles. Ind. J. Biochem. Biophys. 30, 133-134.
Mahady G.B., Pendland S.L., Yun G.S., Lu Z.Z. and Stoia A. (2003). Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of Cag A+ strains of Helicobacter pylori. Anticancer. Res. 23, 3699-3702.
Mallikarjuna K., Chetan P.S., Reddy K.S. and Rajendra W. (2008). Ethanol toxicity: rehabilitation of hepatic antioxidant defense system with dietary ginger. Fitoterapia. 79, 174-178.
Ozata M., Mergen M., Oktenli C., Aydin A., Sanisoglu S.Y., Bolu E., Yilmaz M.I., Sayal A., Isimer A. and Ozdemir I.C. (2002). Increased oxidative stress and hypozincemia in male obesity. Clin. Biochem. 35, 627-631.
Placer Z.A., Cushman L.L. and Johnson B.C. (1966). Estimation of product of lipid peroxidation (malondialdehyde) in biochemical systems. Anal. Biochem. 16, 359-364.
Rababah T.M., Hettiarachchy N.S. and Horax R. (2004). Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola, and ginkgo extracts, vitamin E, and tert-butylhydroquinone. J. Agric. Food Chem. 52, 5183-5186.
Roberts C.K. and Sindhu K.K. (2009). Oxidative stress and metabolic syndrome. Life. Sci. 84, 705-712.
SAS Institute. (1998). SAS User’s Guide: Statistics. SAS Institute Inc. USA.
Zhang G.F., Yang Z.B., Wang Y., Yang W.R., Jiang S.Z. and Gai G.S. (2009). Effects of ginger root (Zingiber officinale) processed to different particle sizes on growth performance, antioxidant status, and serum metabolites of broiler chickens. Poult. Sci. 88, 2159-2166.