Genetic Analysis of Three Structural Proteins in Iranian Infectious Bronchitis Virus Isolate

Document Type : Research Article


Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran


Infectious bronchitis virus (IBV) is a contagious pathogen in fowl that results in economic loss in the poultry industry. In this study, the amino acids sequences of three structural proteins M, N, and S1 for five Iranian IBV isolated during 1998-2011 have been analyzed. Conserved and variable regions, hydrophobic characteristics and identity matrix were determined after alignment by Bioedit ver The phylogenetic tree was obtained by using the neighbor-joining method within MEGA4. Similarity for M and S1 protein was lowest for IR-3654-VM and TW2575 / 98 isolate (0.862) and IR1061-PH and Georgia 1998 isolate / strain (0.41), respectively. For the N protein, highest similarity was found between Ur1 / 09 and Arkansas DPI (0.948). Four conserved regions for M and N proteins were recognized. In the S1 protein three hypervariable regions were detected in 52-90, 124-150 and 265-315 residues. In a phylogeny analysis all proteins were distributed in three clusters. Iranian IBV belonged to Mass cluster in phylogeny tree of M and N proteins. But S1 protein showed a close relationship with the California serotype and was distantly related to Mass serotype. The results showed that the Iranian IBV isolate was probably diverted from Mass strain that might be brought to Iran as a vaccine strain.


Adzhar A.B., Shaw K., Britton P. and Cavannagh D. (1995). Avian infectious bronchitis virus, differences between 793/B and other strains. Vet. Rec. 136-548.
AghakhanS., Afshar Fereidouni M., Marunesi N.A. and Khodashenas M. (1994). Studies on avian viral infections in Iran. Arch. de L, Inst. Razi. 44(45), 1-10.
BeaudetteF.R. and Hudson C.B. (1937). Cultivation of the virus of infectious bronchitis. J. Am. Vet. Med. Assoc. 90, 51-58.
ChenH., Gill A., Dove B.K., Emmett S.R., Kemp C.F., Ritchie M.A., Dee M. and Hiscox J.A. (2005). Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. J. Virol. 79, 1164-1179.
ChenH.W., Huang Y.P. and Wang C.H. (2010). Identification of intertypic recombinant infectious bronchitis viruses from slaughtered chickens. Poult. Sci. 89, 439-446.
ClewleyJ.P., Morser J., Avery R.J. and Lomniczi B. (1981). Oligonucleotide fingerprinting of the RNA of different strains of infectious bronchitis virus. Infect. Immun. 32, 1227-1233.
CorseE. and Machamer C.E. (2003). The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction. Virology. 312, 25-34.
HaqshenasG., Assasi K. and Akrami H. (2005). Isolation and molecular characterization of infectious bronchitis virus isolate Shiraz 3. IBV by RT-PCR and restriction enzyme analysis. Iran J. Vet. Res. 6(2), 9-15.
JayaramJ., Youna S. and Collisson E.W. (2005). The virion N protein of infectious bronchitis virus is more phosphorylated than the N protein from infected cell lysates. Virology. 339, 127-135.
KustersJ.G., Niesters H.G.M., Lenstra J.A., Horzinek M.C. and Van Der Zeijst B.A.M. (1989). Phylogeny of antigenic variants of avian Coronavirus IBV. Virology. 169, 217-221.
LaiM.C. (1992). RNA recombination in animal and plant viruses. Microbial. Rev. 56, 61-79.
LeeS.k., Sung H.W. and Kwon H.M. (2004). S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea. Arch. Virol. 149, 481-494.
LinlinL., Chunyi X., Feng C., Jianping Q., Qingmei X., Yingzuo B. and Yongchang C. (2010). Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in south China during 2004-2008. Vet. Microbiol. 143, 145-154.
LiuH.J., Lee L.H., Shih W.L., Lin M.Y. and Liao M.H. (2003). Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis. J. Virol. Methods. 109, 31-37.
MaedaJ., Repass J.F., Maeda A. and Makino S. (2001). Membrane topology of coronavirus E protein. Virology. 281, 163-169.
MichaelA.J., Catherine P., Jagoda I. and Scott G.T. (2003). A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus. Vaccine. 21, 2730-2736.
NdifunaA., Waters A.K., Zhou M. and Collisson E.W. (1998). Recombinant nucleocapsid protein is potentially an inexpensive, effective sero-diagnostic reagent for infectious bronchitis virus. J. Virol. Methods. 70, 37-44.
Park J.Y., Pak S.I., Sung H.W., Kim J.H., Song C.S., Lee C.W. and Kwon H.M. (2005). Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea. Virus Genes. 31(2), 153-162.
ParkerM.M. and Masters P.S. (1990). Sequence comparison of the N genes of 5 strains of the Coronavirus mouse hepatitis-virus suggests a 3 domain-structure for the nucleocapsid protein. Virology. 179, 463-468.
RenX., Yin J., Ma D. and Li G. (2009). Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06. Virus Genes. 38, 39-45.
RoussanD.A., Ghassan Y.K. and Shaheen I.A. (2009). Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection. Can. Vet. J. 50, 77-80.
SapatsS.I., Ashton F., Wright P.J. and Ignjatovic J. (1996). Sequence analysis of the S1 glycoprotein of infectious bronchitis viruses: identification of a novel genotypic group in Australia. J. Gen. Virol. 77, 413-418.
ShenX., Xue J.H., Yu C.Y., Luo H.B., Qin L., Yu X.J., Chen J., Chen L.L., Xiong B., Yue L.D., Cai J.H., Shen J.H., Luo X.M., Chen K.X., Shi T.L., Li Y.X., Hu G.X. and Jiang H.L. (2003). Small envelope protein E of SARS: cloning, expression, purification, CD determination, and bioinformatics analysis. Acta. Pharmacol. Sin. 24(6), 505-511.
ShoshtariA.H., Toroghi R., Momayez R. and Pourbakhsh S.A. (2008). 793 / B type, the predominant circulating type of avian infectious bronchitis viruses 1999-2004 in Iran: a retrospective study. Arch. Razi Inst. 63(1), 1-5.
SpencerK.A. and Hiscox J.A. (2006). Characterization of the RNA binding properties of the coronavirus infectious bronchitis virus nucleocapsid protein amino-terminal region. FEBS. Letters. 580, 5993-5998.
Wang C.H. and Huang Y.C. (2000). Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus. Arch. Virol. 145, 291-300.
WangL., Junker D., Hock L., Ebiary E. and Collisson E.W. (1994). Evolutionary implications of genetic variations in the Sl gene of infectious bronchitis virus. Virus. Res. 34, 327-338.
WilliamsA.K., Wang L., Sneed L.W. and Collisson E.W. (1992). Comparative analysis of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronavirus. Vi-rusRes. 25, 213-222.
XuC.J., Zhao X. and Zhang H.G. (2007). Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene. Vet. Microbiol. 122, 61-71.
ZhangP., Zhao S., Wang H., Zeng Z., Feng L., Liu Y. and Cao H. (2010). Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the avian infectious bronchitis virus. Afr. J. Biotechnol. 9(49), 8398-8404.
ZhouM. and Collisson E.W. (2000). The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3' genomic RNA. Virus. Res. 67, 31-39.