Myostatin Gene Polymorphism and Its Association with Production Traits in Western Azerbaijan Native Chickens

Document Type: Research Article

Authors

1 Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

In the present study, the polymorphism of myostatin gene (MSTN) in native chickens of Western Azerbaijan Rearing and Breeding Institute was investigated. The blood samples were collected from eighty two randomly selected hens. Genomic DNA was extracted from blood samples and a fragment of myostatin including 599 bp in promoter and exon 1 was amplified using PCR method. Breeding values for body weight and carcass traits were predicted by univariate animal mixed model analysis, using WOMBAT software. The effects of different SSCP genotypes on breeding and phenotypic values of the studied traits were evaluated by general linear model analysis. Three different single strand conformational polymorphism(SSCP) genotypes as AA, AB and AC were identified, with frequencies of 0.244, 0.549 and 0.207, respectively. Shannon and Nei gene diversity indices and number of effective alleles in the studied population were 0.88, 0.53 and 2.2, respectively, which indicated a high diversity of the studied population. Moreover, the studied population was not in Hardy-Weinberg equilibrium. The effect of the SSCP genotypes on breeding and phenotypic values was significant only in the case of breeding value for body weight at 12 weeks of age, whereas, the AC genotype individuals, significantly (P<0.05) had the lowest breeding value for body weight at 12 weeks of age. Based on the results obtained, it could be concluded that the studied fragment of myostatin gene is polymorphic in native chickens of Azerbaijan and could be used for marker assisted selection.

Keywords


Aljanabi S.M. and Martinez I. (1997). Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucl. Acid. Res. 25, 4692-4693.
Baron E.E., Wenceslau A.A., Alvares L.E., Nones K., Ruy D.C., Schmidt G.S., Zanella E.L., Coutinho L.L. and Ledur M.C. (2002). High level of polymorphism in the myostatin chicken gene. Pp. 19-23 in Proc. 7th World Congr. Genet. Appl. Livest. Prod. Montpellier, France.
Barroso A., Dunner S. and Canon J. (1998). Detection of bovine kappa casein variants A, B, C and E by means of polymerase chain reaction- single strand conformation polymorphism. J. Anim. Sci. 76, 1535-1538.
Bassam B.J., Caetano-Anolles G. and Gresshoff P.M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196, 80-83.
Grobet L., Poncelet D., Royo L.J., Brouwers B., Pirottin D., Michaux C., Ménissier F., Zanotti M., Dunner S. and Georges M. (1998). Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double muscling in cattle. Mammal. Genome. 9, 210-213.
Gu Z., Zhang H., Zhu D. and Li H. (2002). Single nucleotide polymorphism analysis of the chicken myostatin gene in different chicken lines. Yi Chuan. Xue. Bao. 29, 599-606.
Kambadur R., Sharma M., Smith T.P.L. and Bass J.J. (1997). Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Res. 7, 910-915.
Karim L., Coppieters W., Grobet L., Valentini A. and Georges M. (2000). Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay. Anim. Genet. 31, 396-399.
Marchitelli C., Savarese M.C., Crisa A., Nardone A., Marsan P.A. and Valentini A. (2003). Double muscling in Marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene. Mammal. Genome. 14, 392-395.
Mc Croskery S., Thomas M., Maxwell L., Sharma M. and Kambadur R. (2003). Myostatin negatively regulates satellite cell activation and self-renewal, J. Cell. Biol. 162, 1135-1147.
Mc Pherron A.C., Lawler A.M. and Lee S.J. (1997). Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 387, 83-90.
Mc Pherron A.C. and Lee S.J. (1997). Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. 94, 12457-12461.
Meyer K. (2009). Wombat a program for mixed model analyses by restricted maximum likelihood. at: http://agbu.une.edu.au/~kmeyer/download.php? file= Wombat Manual.pdf.
Orita M., Iwahana H., Kanazawa H., Hayashi K. and Sekiya T. (1989a). Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. 86, 2766-2770.
Orita M., Suzuki Y., Sekiya T. and Hayashi K. (1989b). Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics. 5, 874-879.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC.
Thomas M., Langley B., Berry C., Sharma M., Kirk S., Bass J. and Kambadur R. (2000). Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation.J. Biol. Chem. 275, 40235-40243.
Ye X., Brown S.R., Nones K., Coutinho L.L., Dekkers J.C. and Lamont S.J. (2007). Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens. Genet. Sel. Evol. 39, 73-89.
Yeh F.C., Yang R.C., Boyle T.B.J., Ye Zh. and MaoJ.X. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Canada.
Zhang G., Ding F.,Wang J.,Dai G.,Xie K.,Zang L.,Wang W. and Zhou S.h. (2011). Polymorphism in exons of the myostatin gene and its relationship with body weight traits in the Bian chicken. Biochem. Genet. 49, 9-19.
Zhiliang G., Dahai Z., Ning L., Hui L., Xuemei D. and Changxin W. (2004). The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth. Sci. China C. Life Sci. 47, 25-30.