Semen Ejaculates Characteristics, in vitro Fertility and Their Interrelationships in Sahiwal Bull Semen

Document Type : Research Article

Authors

Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Science, WBUAFS, 37, K.B. Sarani, Kolkata, 700037, West Bengal, India

Abstract

This study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of Sahiwal bulls. Semen samples werecollected weekly for 6 weeks from ten mature Sahiwal bulls divided randomly into 5 groups. The mean (±SEM) of ejaculate volume (mL), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/mL), live sperm (%), abnormal sperm (%), intact acrosome (%), hypo-osmotic swelling (HOS) positive sperm (%), sperm penetration distance (SPD, mm) were 4.28, 3.17, 76.73, 1185.53, 83.37, 9.97, 82.70, 79.00 and 56.85, respectively. The bulls varied significantly (P<0.05) in all their ejaculates characteristics Moreover, HOS positive sperm also had significant (P<0.01) positive correlation with mass activity (r=0.756), progressively motile sperm (r=0.374), live sperm (r=0.395) and SPD (r=0.681), and negative correlation with abnormal sperm (r=-0.463). Sperm penetration distance has significant (P<0.05) positive correlation with mass activity (r=0.776), progressively motile sperm (r=0.653), sperm concentration (r=0.365) and HOS positive sperm (r=0.681), and negative correlation with intact acrosome (r=-0.415).Overall semen quality of Sahiwalbulls was found optimum for use in breeding programme.

Keywords


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