1Department of Veterinary Biochemistry and Physiology, GBPUA and T Pantnagar, Uttarakhand, India
2Department of Veterinary Gynaecology and Obstetrics, GBPUA and T Pantnagar, Uttarakhand, India
Receive Date: 02 March 2012,
Revise Date: 11 April 2012,
Accept Date: 01 May 2012
The present work was carried out to study the variation in biochemical properties of cervico-vaginal mucus of pregnant and non pregnant animals which could help to design suitable tool in future for early pregnancy diagnosis in buffaloes. Cervico-vaginal mucus samples were obtained from 30 buffaloes (12 pregnant and 18 non pregnant) and some biochemical parameters were compared. Means of pH, sodium, potassium and chloride had no significant difference between the estrous cycle and pregnancy. Total sugar content showed significant differences in pregnant and non pregnant animals from 6th to 21st day (P<0.05). In pregnant animals, continuously increasing trend was observed from day 0 to day 21 (25.22±0.5 mg/100 mL on day 0 to 130.5±1.1 mg/100 mLon day 6 and later 156.4±1.8 mg/100 mL on day 21) whereas in non pregnant animals the concentration of total sugar increased from day 0 to day 12 and then started decreasing (24.33±0.6 mg/100 mL on day 0, 118.1±1.7 mg/100 mLon day 6, 127.9±1.5 mg/100 mL on day 12 and then decreased to 90.8±1.2 mg/100 mLon day 21). Protein content of vaginal mucus was different in pregnant and non pregnant animals from 12 to 21 day of experiment. A continuously decreasing trend of protein content was observed in non pregnant group after 12th day (14.3±0.43 mg/mL on day 12 and 9.9±0.55 mg/mL on day 21) whereas in pregnant group, the concentration remained higher up to 21st day without any significant change (26.8±0.33 mg/mL on day 12 to 29.6±0.12 mg/mL on day 21). However, protein content initially increased in both pregnant (42.4±0.22 mg/mL) and non pregnant (40.2±0.03 mg/mL) group and showed highest values on third day. Consequently, significant variation in total sugar and protein in cervico-vaginal mucus could be helpful in designing future biochemical tool for early pregnancy diagnosis in buffalo.
Bane A and Rajakoski E. (1961). The bovine estrous cycle. Cornell Vet.51, 77-95. Bhatia S. (2007). Electrolytes of cervico-vaginal mucus and blood during estrous cycle in normal and repeat breeding Rathi cows. Indian J. Anim. Sci. 53, 66-68. El-Naggar M.A. and Baksai Horvath E. (1975). Biochemical changes in the cervico-vaginal mucus of infertile cows. Act. Vet. Acad. Sci. Hung.22, 31-35. Lowry C.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275. Peterson G.L.A. (1977). Simplification of proein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346-356. Prasad A., Kalalyan N.R., Bachlaus N.K., Arora R.C. and Pandey R.S. (1981). Biochemical changes in the cervical mucus of buffalo after induction of oestrous with prostaglandin F2a and chlorprostenol. J. Reprod. Fertil.62, 583-587. Roughan P.G. and Batt R.D. (1968). Quantitative analysis of sulpholipid (sulfo-quinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissue. Biochem. J.73, 217-225. Sood P., Vasishta N.K., Singh M.M. and Nigam J.M. (1950). Relationship of certain biochemical attributes in cervical mucus with conception rate in cows. Indian J. Anim. Reprod. 21, 57-58. Tsiligianni T.H., Karagiannidis A. and Saratsis P.H. (2001). Chemical properties of bovine cervical mucus during normal estrus and estrus induced by progesterone and / or PGF2a. Theriogenology.56, 41-50. Zaaijer D., Counotte G.H., Sol J.J., Smidt W.J. and Broadbent P.J. (1993). Changes in the composition of cervical mucus of the cow during the estrous cycle as parameters for predicting potential fertility. Theriogenology.39, 569-580.