Lack of Association between Somatotropin Receptor Gene Polymorphism and Birth Weight of Iranian Indigenous Sistani Cattle

Document Type: Research Article

Authors

1 Department of Animal Science, University of Tehran, Karaj, Iran

2 Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran

Abstract

The objective of the present study was to determine polymorphism within the promoter region of somatotropin receptor genes in indigenous Sistani cattle (Bos indicus) and associations between this polymorphism and breeding value of birth weight. The pedigree structure was included by considering 1173 animals with 600 progeny birth weight data obtained from a Zhark breeding station in Sistan and Baluchistan. Heritability was estimated for birth weight using different univariate models with the derivative-free approach of restricted maximum likelihood algorithm (DFREML). The average weight of each birth was 23.9 ± 3.16 kg. The effects of non-genetic factors were significant (P<0.01) for birth weight. Direct heritabilities (h2) in single trait analyses were 0.31 ± 0.06. Genomic DNA was isolated from blood and semen using conventional methods. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype this candidate gene in 72 individuals with the birth weight record. The observed allele size was similar to that reported in the literature. The Sistani cattle showed higher frequency of alleles ALuI (+) than ALuI (-) in population. Statistical analysis was conducted to test the association of this polymorphism with the breeding value of birth weight data. There was not significant association between producing genotypes and birth weight trait. Future research on another candidate gene and growth trait strongly is encouraged to deep in the understanding of growth pattern in this breed.

Keywords


Aggrey S.E., Yao S., Sabour M.P., Lin C.Y., Zadworny D., Hayes J.F. and Kuhnlein U. (1999). Markers within the regulatory region of the growth hormone receptor gene and their association within milk-relater in Holstein. J. Her. 90(1), 148-151.
Assan N., Dube B., Ngwenya S., Nyoni K., Tambo G. and Matshe F. (2011). Sex specific genetic and environmental trends for birth weight in indigenous Nguni conservation cattle herd of Zimbabwe. J. Cell Anim. Biol. 5, 20-26.
Bayram B. and Aksakal V. (2009). Estimates of genetic and phenotypic parameters for the birth weight of calves of Holstein Friesian cattle reared organically. J. Anim. Vet. Adv. 8, 568-572.
Falaki M., Gengler N., Sneyers M., Prandi A., Massart S., Formigoni A., Burny A., Portetelle D. and Renaville R. (1996). Relationship of polymorphisms of growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci. 79, 1446-1453.
Ge W.M., Davis E., Hines H.C. and Irvin K.M. (1999). Two-allelic DGGE polymorphism detected in the promoter region of the bovine GHR gene. Anim. Gen. 30, 71.
Habib M.A., Bhuiyan A.K.F.H. and Amin M.R. (2009). Birth weight and its non-genetic effect in red Chittagong cattle (RCC) in a closed nucleus herd. Int. J. Biol. Res. 1, 35-39.
Harvey S., Scanes C.G. and Daughadary W.H. (1994). Growth Hormone. CRC Press, London.
Heap D., Lucy M.C., Collier R.J., Boyd C.K. and Warren W.C. (1995). Nucleotide sequence of the promoter and first exon of the somatotropin receptor gene in cattle. J. Anim. Sci. 37, 1529.
Kaygisiz A., Bakir G. and Vanli Y. (2010). Estimation of variance components and genetic parameters for direct and maternal effects on birth weight in brown swiss. Pakistan Vet. J. 31, 70-74.
Lucy M.C., Johnson G.S., Shibuya H., Boyd C.K. and Herring W.O. (1998). Polymorphic (GT) n microsatellite in the bovine somatotropin receptor gene promotor. J. Anim. Sci. 76, 2209-2210.
Meyer K. (2002). Programms to Estimate Variance Components for Individual Animal Models by Restricted Maximum Likelihood (REML) Ver. 3.1.0. Users Notes. Inst. Anim. Sci., Armidale.
Misio S., Elo K., Kantanen J. and Vilkki J. (1998). Polymorphism within the 3-flanking region of the bovine growth hormone receptor gene. Anim. Gen. 29, 55-57.
Moody D.E., Pomp D. and Barends W. (1995b). Restriction fragment length polymorphism in amplification products of the bovine growth hormone releasing hormone gen. J. Anim. Sci. 73, 3789-3793.
Olawumi S.O. and Salako A.E. (2010). Genetic parameters and factors affecting reproductive performance of white Fulani cattle in southwestern, Nigeria. Global Vet. 5, 255-258.
Raja T.V., Venkatachalapathy R.T. and Kannan A. (2010). Estimates of genetic and phenotypic parameters on birth weight of crossbred cattle raised under organized farm conditions. J. Anim. Vet. Adv. 9, 2275-2278.
SAS. (2009). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC.
Shahzad F., Yaqoob M., Younas M., Farooq U. and Sher F. (2010). Factors affecting the birth weight of cholistani cattle calves. Pakistan. Vet. J. 30, 247-248.
Tavakolian J. (2009). An Outlook toward the Livestock and Poultry Genetic Resources of Iran. Animal Sciences Research Institute, Karaj, Iran (in Persian).
Topal M., Aksakal V., Bayram B. and Yaganoglu A.M. (2010). An analysis of the factors affecting birth weight and actual milk yield in Swedish red cattle using regression tree analysis. J. Anim. Plants. Sci. 20, 63-69.
Unalan A. (2009). Estimation of genetic parameters and correlations among some body measurements of Holstein calves and effects of these measurements on calving difficulty. J. Anim. Vet. Adv. 8, 1589-1594.
Van der Werf J.H.J., Marshall K. and Lee S. (2007). Methods and experimental designs for detection of QTL in sheep and goats. Small Rumin. Res. 70(1), 21-31.
Yeh F.C., Yang R.C., Boyle T.B.J., Ye Z.H. and Mao J.X. (1997). PopGene, the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.