1Department of Animal Science, Faculty of Agriculture, GonbadUniversity, Gonbad, Iran
2Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
Receive Date: 24 September 2012,
Revise Date: 23 November 2012,
Accept Date: 31 December 2012
In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (SSCP) method on polyacrylamide gel. The results indicated that there are four (G1, G2, G3 and G4) conformational patterns with frequencies 0.38, 0.21, 0.23 and 0.18 for Alpine goat and 0.48, 0.21, 0.17 and 0.14 for Saanen goat, respectively. These results revealed that GH gene was polymorph in this studyand showed that PCR-SSCP is an appropriate tool for detecting polymorphism and evaluating genetic variability.
Ahani Azari M., Yousefi S. and Dehnavi E. (2011). Evaluation of K-casein and growth hormone genes polymorphism in native Dalagh sheep. Slovak J. Anim. Sci. 44, 129-133. American Dairy Goat Association. (2012). Available at: http://www.adga.org. htm Accessed Jan. 2012. Ayuk J. and Sheppard M.C. (2006). Growth hormone and its disorders. Postgrad. Med. J. 82, 24-30. Baldi A. (1999). Manipulation of milk production and quality by use of somatotropin in dairy ruminants other than cow. Domest. Anim. Endocrinol. 17, 131-137. Barracosa H. (1996). Estudo de polimorfismos genéticos eda sua associação com capacidades de pro dução leiteira em caprinos de raça Algarvia e ovinos de raça Serra da Estrela. MS Thesis. de Mestrado, Univ., do Algarve, Faro, Portugal. Bastos E., Cravador A., Azevedo J. and Guedes Pinto H. (2001). Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breeds “Churra da Terra Quente”. Biotech. Agron. Soc. Environ. 5, 7-15. Bauman D.E. (1999). Somatotropin mechanism in lactating cows: from basic science to commercial application. Domes. Anim. Endocrinol. 17, 101-116. BreierB.H. (1999). Regulation of protein and energy metabolism by the somatotropic axis. Domes. Anim. Endocrinol. 17, 209-218. Gootwine E., Suttie J.M., McEwan J.C., Veenvliet B.A., Littlejohn R.P., Fennessy P.F. and Montgomery G.W. (1998). The physiological efects of natural variation in growth hormone gene copy number in ram lambs. Domest. Anim. Endocrinol. 14, 381-390. Gupta N., Ahlawat S.P.S., Kumar D., Gupta S.C., Pandey A. and Malik G. (2007). Single nucleotide polymorphism in growth hormone gene exon-4 and exon-5 using PCR-SSCP in Black Bengal goats A prolific meat breed of India. Meat Sci. 76, 658-665. Lagziel A., Lipkin E. and Soller M. (1996). Association between SSCP haplotypes at the bovine growth hormone gene and milk protein percentage. Genet. 142, 945-951. Ling Jiang M., Mei Yu L., Qiang S.G., Qing Jie P.and Hong C.(2005). Relationship between polymorphism of growth hormone gene and production traits in goats. Act.Genet.Sini. 32, 650-654. Malveiro E., Pereira M., Marques P.X., Santos I.C., Belo C., Renaville R. and Cravador A. (2001). Polymorphisms at the five exons of the growth hormone gene in the Algarvia goat: possible association with milk traits. Small Rumin. Res. 41, 163-170. Marques M.R., Santos I.C., Belo C.C. and Cravador A. (2001). Associations between SSCPs in the GH gene and milk traits in “Serra da Estrela” ewes. .Pp57-82 in Proc. 4th Int. Conf., Farm Anim. Endocrinol., Gembloux, Belgium. Marques P.X., Pereira M., Marques M.R., Santos I.C., Belo C.C., Renaville R. and Cravador A. (2003). Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Rumin. Res. 50, 177-185. Miller S.A., Dykes D.D. and Polesky H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucl. Acid. Res. 16(3), 12-15. Mousavizadeh A., Mohammad Abadi M., Torabi A., Nassiry M.R., Ghiasi H. and Koshkoieh A.E. (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian J. Biotech. 7(1), 51-53. Ofir R. and Yossefi S. (1996). Characterization of PvuII polymorphisms between the ovine growth hormone GH2-N and GH2-Z gene copies. Anim. Biotech. 7, 135-143. Scaramuzzi R.J., Murray J.F., Downing J.A. and Campbell B.K. (1999). The effects of exogenous growth hormone on follicular steroid secretion and ovulation rate in sheep. Domes. Anim. Endocrinol. 17, 269-277. Shiri S.A.K., Saghi D.A., Nasiri M.R., Emrani H., Montazer Torbati F. and Mohammadzade M. (2006). Survey of genetic diversity growth hormone and growth hormone receptor genes in Iranian indigenous sheep breed (Kordian sheep) using a non-radioactive SSCP. Pp. 21-28 in Proc. 57th Ann. Meet. Europ. Asso. Anim. Prod. Antalya, Turkey. Tahmorespoor M., Taheri A., Gholami H. and Ansary M. (2011). PCR-SSCP variation of GH and STAT5A genes and their association with estimated breeding values of growth traits in baluchi sheep. Anim. Biotechnol. 22(1), 37-43. Zhang C., Liu Y., Huang K., Zeng W., Xu D., Wen Q. and Yang L. (2011). The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds.Genet. Mol. Biol. 34(1), 49-55.