Intestinal Histomorphology Changes and Serum Biochemistry Responses of Broiler Chickens Fed Herbal Plant (Euphorbia hirta) and Mix of Acidifier

Document Type : Research/Original Article


1 Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

2 Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia

3 Department of Microbiology and Immunology, Golestan University of Medical Science, Gorgan, Iran

4 Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia


The aim of this study was to evaluate the effect of dietary Euphorbia hirta and an acidifier mixture supplementation on gut morphology and some blood parameters of broiler chickens.A total of 240 day old male broiler chicks were randomly assigned to one ofthe four dietary treatment groups including: (1) basal diet (control), (2) basal diet + 7.5 g/kg E. hirta (Eh7.5), (3) basal diet + 1.5 g/kg acidifier (OA) and (4) basal diet + 7.5 g/kg E. hirta and1.5 g/kg acidifier (EhOA). The Eh 7.5, OA and EhOA supplementation significantly improved overall feed conversion ratio compared to the control group. The addition ofEh 7.5, OA and their combination increased the villus height compare to the control birds. Crypt depth was markedly decreased by OA treatment. The highest ratio of villi to crypt was observed in OA fed broilers. Blood serum biochemical parameters did not influenced by the dietary treatments. In conclusion, the results indicated thataddition of Eh 7.5 and OA to the broiler diet enhanced maintenance and function of the small intestine and broiler performance.


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  • Receive Date: 23 September 2012
  • Revise Date: 29 November 2012
  • Accept Date: 12 February 2013
  • First Publish Date: 01 March 2014