Association of Melatonin Receptor 1A Gene Polymorphisms with Production and Reproduction Traits in Zandi Sheep

Document Type: Research Article

Authors

Department of Animal Science, Faculty of Animal Science and Fisheries, Sari University of Agricultural Science and Natural Resources, Sari, Iran

Abstract

Melatonin regulates some major physiological processes such as maturation and function of reproductive system, pubertal development, seasonal reproduction and adaptation. The activation of melatonin hormone is mediated by melatonin receptor. Previous studies showed melatonin receptor 1A gene (MTNR1A) is highly polymorphic in seasonally breeding species. The aim of the present study was detection of MTNR1Apolymorphism and its association with production (body weights at birth, 1, 3, 6, 9 and 12 month of age) and reproduction (litter size) traits in Zandi sheep using PCR-RFLP methodology. Hundred blood samples were randomly collected and genomic DNA was isolated using modified salting out method. A large fragment of exon 2 of MTNR1A gene was amplified by PCR using specific primer pairs. After amplification, the PCR product was digested with MnII endonuclease. Restricted digestion allowed the determination of two alleles (M, m) and two genotypes (MM, Mm) with frequencies of 0.91, 0.09 and 0.82, 0.18, respectively. Least square means showed that MM individuals had higher body weight at one month of age (BW1) than Mm individuals (P<0.05). No associations were found between observed genotypes and other studied traits.In the subsequent studies using a large number of samples along with the other important parameters such as sire effect and the lamb health status which may influence lamb body weight is recommended.

Keywords


Barrett P., Conaway S., Jockers R., Strosberg A.D., Guardiola-Lemaitre B., Delagrange P. and Morgan P.J. (1997). Cloning and functional analysis of a polymorphic variant of the Ovine mella melatonin receptor. Biochim. Biophys. Acta. 1356, 299-307.
Bartness T., Bittman E.L., Hastings M.H., Powers J.B. and Goldman B. (1993). Timed melatonin infusion paradigm for melatonin delivery: what has it taught us about the melatonin signal, its reception and the photoperiodic control of seasonal responses? J. Pineal Res. 15,161-190.
Chu M.X., Ji C.L. and Chen G.H. (2003). Association between PCR-RFLP of melatonin receptor 1a gene and high prolificacy in Small Tail Han sheep. Asian-australas J. Anim. Sci. 16, 1701-1704.
Chu M.X., He Y.Q., Cheng D.X., Ye S.C., Fang L. and Wang J.Y. (2007). Association between expression of reproductive seasonality and alleles of melatonin receptor 1A in goats. Anim. Reprod. Sci. 101, 276-284.
Edyta M., Genowefa B., Aneta Z., Tomasz M., Henryk P. and Dorota Z. (2011). Effect of day length and exogenous melatonin on chemical composition of sheep milk. Arch. Tierzucht.  54(2), 177-187.
Gall C.V., Stehle G.H. and Weaver D.R. (2002). Mammalian melatonin receptors: molecular biology and signal transduction. Cell Tiss. Res. 309, 151-162.
GhiasI H., Nasiry M.R., Mousavi A.R.H., Mousavizadeh A.A. and Javad Manesh A.(2006). Genetic polymorphism of the melatonin receptor 1A locus in Iranian Shall and Karakul sheep. Iran J. Biotechnol.  4(3), 201-203.
Hernandez X., Bodin L., Chesneau D., Guillaumw D., Chemineau P., Malpaux B. and Migaud M. (2005). Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Île-de-France ewes. Reprod. Nutr. Dev. 45, 151-162.
Kaczor U., kamiecik M., Molik E. and Rycheusz T. (2006). Polymorphism in the melatonin receptor gene MT1 (locus MTNR1A) in sheep. Arch. Tierz. 49, 257-262.
Lincoln G.A. and Ebling F.J.P. (1985). Effect of constant release implants of melatonin on seasonal cycles in reproduction, prolactin secretion and moulting in rams. J. Reprod. Fertil.  73, 241-253.
Malpaux B., Migaud M., Tricoire H. and Chemineau P. (2001). Biology of mammalian photoperiodism and the critical role of the pineal gland and melatonin. J. Biol. Rhythms. 16, 336-347.
Messer A.L., Wang L., Tuggle C.K. and Rothschild M.F. (1997). Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep and cattle. Mammal. Genome. 8, 368-370.
Mura M.C., Luridiana S., Vacca G.M., Bini P.P. and Carcangiu V. (2010). Effect of genotype at the MTNR1A locus and melatonin treatment on first conception in Sarda ewe lambs. Thriogen. 74,1579-1586.
Notter D.R., Cockett N.E. and Hadfield T.S. (2003). Evaluation of melatonin receptor 1a as a candidate gene influencing reproduction in an autumn-lambing sheep flock. J. Anim. Sci. 81, 912-917.
Pelletier J., Bodin L., Hanocq E., Malpaux B., Teyssier J., Thimonier J. and Chemineau P. (2000). Association between expression of reproductive seasonality and alleles of the gene for Mella receptor in the ewe. Biol. Reprod. 62, 1096-1101.
Reppert S.T., Weaverv D.R. and Ebisawa T. (1994). Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron. 13(5), 1177-1185.
SAS Institute. (2000). SAS/STAT User’s Guide: Version 8.1st Edn. SAS Institute Inc., Cary, North Carolina, USA.
Teyssier J., Migaud M., Debus N., Maton C., Tillard E., Malpaux B., Chemineau P. and and Bodin L. (2010). Expression of seasonality in Merinos d’Arles ewes of different genotypes at the MT1 melatonin receptor gene. Animal.  5(3), 329-336.
Trecherel E., Batailler M., Chesneau D., Delagrange P., Malpaux B., Chemineau P. and Migaud M. (2010). Functional characterization of polymorphic variants for ovine MT1 melatonin receptors: possible implicationfor seasonal reproduction in sheep. Anim. Reprod. Sci. 122, 328-334.