1Livestock Genome Analysis Laboratory, National Dairy Research Institute, Karnal, 132001, Haryana State, India
2Department of Animal Genetics and Breeding, Faculty of Veterinary Science, Allahabad Agricultural Institute Deemed University, Allahabad, 211007, Utter Pradesh, India
Receive Date: 24 January 2013,
Revise Date: 13 March 2013,
Accept Date: 16 March 2013
DNA polymorphism within diacylglycerol transferase 2 (DGAT2) / monoacyl glycerol transferases 2 (MOGAT2), leptin and butyrophilin genes were analysed using PCR-SSCP in Murrah buffalo. The single strand conformation polymorphism (SSCP) analysis of amplified gene fragment in exon 5 of MOGAT2, exon 3 of leptin and intron 1 of butyrophilin gene revealed different patterns. A, B and C showed the following frequencies for each candidate gene in 53 (A=0.49, B=0.36 and C=0.15), 65 (A=0.38 and B=0.62) and 55 samples (A=0.6, B=0.31 and C=0.09) from Murrah buffaloes, respectively. The strand conformation polymorphism (SSCP) followed by DNA sequencing revealed one single nucleotide polymorphism (SNP) that was (c.193T>C) in MOGAT2, one single nucleotide polymorphism (SNP) (c.25 T>C) in leptin and one single nucleotide polymorphism (SNP) (c.184C>T>G) in butyrophilin gene confirmed BTI1 SSCP.The statistical analysis using general linear model procedure for association study, indicated that Murrah buffalo monoacyl glycerol transferases 2 (MOGAT2)(c.193T>C) single nucleotide polymorphism (SNP)and (c.25 T>C) in leptingenotypes were not significantly different (P>0.01) in Murrah buffalo for milk production traits milk yield, fat percentage and single nucleotide polymorphism (SNP)percentage. However, the statistical analysis for association study indicated BTI1 SSCP was significantly (P≤0.05) associated with 305-days lactation milk yield. The Murrah buffaloes with BTI1BB genotypes had 683.93 kg and 320.48 kg higher milk yield as compared to BTI1AA and BTI1CC genotypes, respectively. The positive association of butyrophilin single strand conformation polymorphism (SSCP) polymorphism with milk yield will be a useful tool for future selection and breeding strategies and genetic improvement of buffaloes for milk yield.
Block S.S., Butler W.R., Ehrhardt R.A., Bell A.W., Van Amburgh M.E. and Boisclair Y.R. (2001). Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J. Endocrinol.171, 339-348. BorgheseA.M.M. (2005). Buffalo Production and Research.FAO Rome, Regional Office for Europe. Cases S., Smith S.J., Zheng Y.W., Myers H.M., Lear S.R., Sande E., Novak S., Collins C., Welch C.B., Lusis A.J., Erickson S.K. and Farese R.V. (1998). Identification of a gene encoding an acyl-CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Nat. Acad. Sci. 95, 13018-13023. Chamberlain A.J., Hayes B.J., Savin K., Bolormaa S., McPartlan H.C., Bowman P.J., Van Der Jagt C., MacEachern S. and Goddard M.E. (2012). Validation of single nucleotide polymorphisms associated with milk production traits in dairy cattle. J. Dairy Sci. 95(2), 864-875. Clamp P.A., Feltes R., Shalvevet D., Beever J.E., Atac E. and Schook L.B. (1993). Linkage relationship between ALPL, EN01, GPI, PGD, TGFB1 on porcine chromosome 6. Genomics. 17, 324-329. Di Meo G.P., Perucatti A., Uboldi C., Roperto S., Incarnato D., Roperto F., Williams J., Eggen A., Ferretti L. and Iannuzzi L. (2005). Comparative mapping of the fragile histidine triad (FHIT) gene in cattle, river buffalo, sheep and goat by FISH and assignment to BTA22 by RH-mapping: a comparison with HSA3.Anim. Genet.36(4), 363-364. Franke W.W., Heid H.W., Grund C., Winter S., Freudenstein C., Schmid E., Jarasch E.D. and Keenan T.W. (1981). Antibodies to the major insoluble milk fat globule membrane associated protein: specific location in apical regions of lactating epithelial cells. J. Cell. Biol. 89, 485-494. Gallagher D.S.Jr. and Womack J.E. (1992). Chromosome conservation in the Bovidae.J. Hered.83(4), 287-298. Jang G.W., Cho K.H., Kim T.H., Oh S.J., Cheong I.C. and Lee K.J. (2005). Association of candidate genes with production traits in Korean dairy proven and young bulls. Asian-australas J. Anim. Sci.18, 165-169. Liefers S.C., Veerkamp R.F., Te Pas M.F., Delavaud C., Chilliard Y., Platje M. and Van der Lende T. (2005). Leptin promoter mutations affect leptin levels and performance traits in dairy cows. Anim. Genet.36(2), 111-118. Oikonomou G., Michailidis G., Kougioumtzis A., Avdi M. and Banos G. (2011). Effect of polymorphisms at the STAT5A and FGF2 gene loci on reproduction, milk yield and lameness of Holstein cows. Res. Vet. Sci.91, 235-239. Orita M., Suzuki Y., Sekiya T. and Hayashi K. (1989). Rapid and sensitive detection of point mutation and DNA polymorphisms using polymerase chain reaction. Genomics. 5, 874-879. RozenS. and Skaletsky H.J. (1998). Primer 3. Code. available at: http://www.genome.wi.mit.edu/gnome_software/other/primer3.htm. Sambroo kJ. and Russel D.W. (2000). Molecular Cloning: A Laboratory Manual.Cold Spring Harbour, New York. Vallinoto M., Schneider M.P.C., Silva A., Iannuzzi L. and Brenig B. (2004). Molecular cloning and analysis of the swamp and river buffalo leptin gene. Anim. Genet. 35, 462-463. Winter A., Eckeveld Van M., Bininda Emonds O.R.P., Habermann F.A. and Fries R. (2003). Genomic organization of the DGAT2 / MOGAT gene family in cattle (Bos taurus) and other mammals. Cytogen. Genom. Res.102, 42-47. Winter A., Kramer W., Werner F.A., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G. and Fries R. (2002). Association of a lysine-232 / alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Nat. Acad. Sci. 20, 20-27.