Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction

Document Type : Short Communication

Author

Department of Animal Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Abstract

The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from 67.5% (81/120) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48.1% (39/81) of the isolates were determined to have this virulence factor. Correct amplification with a molecular length of 384 bp was obtained in the analysis of all the isolates by species-specific PCR, which confirmed the results of the detection of the eaeA gene in Escherichia coli. The eaeA gene, which is mainly responsible for the virulence of Escherichia coli, is commonly present in Escherichia coli strains isolated from this region and the significance of this situation for animal and public health was discussed.

Keywords


Bi Z., Nagayama K., Akeda Y., Cantareli V., Kodama T., Takarada Y., Shibata S. and Honda T. (1999). Development of an enzymelabeled oligonucleotide probe for detecting the Escherichia coli attaching and effacing A gene. Microbiol. Immunol. 43, 663-667.
Debroy C. and Maddox C.W. (2001). Identification of virulence attributes of gastrointestinal Escherichia coli isolates of veterinary significance. Anim. Health Res. Rev. 2, 129-140.
Gannon V.P.J., Rashed M., King R.K. and Thomas E.J.G. (1993). Detection and characterization of the eae gene of Shiga-like toxin producing Escherichia coli using polymerase chain reaction. J. Clin. Microbiol. 31, 1268-1274.
Kariuki S., Gilks C., Kimari J., Muyodi J., Getty B. and Hart C.A. (2002). Carriage of potentially pathogenic Escherichia coli in chickens. Avian Dis. 46, 721-724.
O’Brien A.D., Holmes R.K. (1987) Shiga and Shiga-like toxins. Microbiol. Rev. 51, 206-220.
Zahraei T., Safarchi A., Peighambari S.M., Mahzoumieh M. and Rabbani M. (2007) Detection of Stx1 eae, espb and hly genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. J. Vet. Res. 62(2), 37-42.
Zakeri A. and Kashefi P. (2012) Isolation, Serotyping and drug resistance patterns of Escherichia coli from cases of colibacillosis in Tabriz. Wulfenia J. 19(9), 244-253.