Relationship among Body Weight, Testicular Traits and Linear Body Measurements of Red Sokoto Bucks FedDifferent Levels of Sabara (Guiera senegalensis) Leaf Meals

Document Type: Research Article

Authors

1 Department of Animal Science, Federal University, Dutse P.M.B. 7156, Jigawa State, Nigeria

2 Department of Animal Science, University of Maiduguri, P.M.B. 1056, Borno State, Maiduguri, Nigeria

3 Department of Animal Science, Kano University of Science and Technology, Wudil, P.M.B. 3244, Kano State, Nigeria

Abstract

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II stage was 61.41 ± 1.97%. The matured COCs were fertilized for 5 hours in Brackett and Oliphant media using fresh and frozen thawed semen separately. After fertilization the oocytes were cultured in TCM-199 for 48 hours to observe the cleavage rate. The maturation, fertilization and culture were performed in an incubator at 38.5 ˚C with 5% CO2 in humidified air. After fertilization cleavage rates were observed to check the fitness of zygotes to be morula and blastocyst. It was observed that the rates of normal fertilization (2 PN formations) for fresh and frozen semen were 36.02 ± 2.79 and 34.73 ± 2.58%, respectively and the cleavage rates were 25.19 ± 2.5 and 21.01 ± 2.8%, respectively. No significant differences (P>0.05) was observed between fresh and frozen semen in the efficiency of in vitro fertilization and subsequent development of goat embryos. It can be concluded that, both fresh and frozen semen can be used for IVF and subsequent development of goat embryos.

Keywords


Baldassarre H. and Karatzas C.N. (2004). Advanced assisted reproduction technologies (ART) in goats. Anim. Reprod. Sci. 82, 255-266.
Ball G.D., Leibried M.L., Ax R.L. and First N.L. (1984). Maturation and fertilization of bovine oocytes in vitro. J. Dairy Sci. 67, 2775-2785.
Brackett B.G., Bousquet D., Boice M.L., Donawick W.J., Evans J.F. and Dressel M.A. (1982). Normal development following in vitro fertilization in the cow. Biol. Reprod. 27, 147-158.
Carolon C., Monaghan P., Mehmood A., Lonergan P., Gallagher M. and Gordon I. (1992). Slicing of bovine ovaries as a means of oocyte recovery. J. Reprod. Fertil. 9, 51-52.
Danilda H. (2000). Technical aspect of in vitro embryo production. Duran Embryo Biotechnology Laboratory, Philippine Carabao Center, National Headquarters abd Gene Pool Munoz, Nueva Ecija 3120 Phillipine.
De S.V., Ahmed A.M., Martino A. and Cognie Y. (1992). In vitro maturation and fertilization of goat oocytes. Theriogenology. 37, 1049-60.
FAO. (2003). Food and Agriculture Organization of United Nations, Rome, Italy.
Ferdous J. (2006). Collection, grading and evaluation of goat cumulus-oocyte-complexes (COCs) in view of in vitro maturation, fertilization and culture. MS Thesis. Dep. Anim. Breed. Genetic.Bangladesh Agricultural Univ., Mymensingh.
Fuki Y. and Ono H. (1989). Effect of sera, hormones and granulosa cells added to culture media for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil. 86, 501-506.
Ge-Li JunL.Q., Gao-Jun Feng C.A.Q. and Zheng Xiao Feng R.R. (2009). Effect of semen quality on in vitro production of porcine embryos. Act. Agric. Shangha. 25(3), 6-9.
Hafez E.S.E. (1993). Reproduction in Farm Animals. Lea and Febriger. Philadelphia.
Holtz W. (2005). Recent developments in assisted reproduction in goats. Small Rum. Res. 60, 95-110.
Hoque S.A.M. (2009). Effect of collection techniques and goat follicular fluid onin vitro maturation and fertilization of oocytes. MS Thesis. Dep. Anim. Breed. Genetic. Bangladesh Agricultural Univ., Mymensingh.
Islam M.R., Khandoker M.A.M.Y., Afroz S., Rahman M.G.M. and Khan R.I. (2007). Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. J. Zhe. Univ. 8, 465-469.
KhandokerM.A.M.Y., Imai K., Takahashi T. and Hashizume K. (2001). Role of gelatinase on follicular atresia in the bovine ovary. Biol. Reprod. 65, 720-732.
Mondal A., Khandoker M.A.M.Y., Rahman A.H.M.S., Apu A.S. and Pervage S. (2008). In vitro production of goat embryos. Bang. J. Anim. Sci. 37, 1-9.
Pawshe C.H., Totey S.M. and Jain S.K. (1994). A comparison of three methods of recovery of goat oocytes for in vitro maturation and fertilization. Theriogenology. 42, 117-125.
Rho G.J., Hahnel A.C. and Betteridge K.J. (2001). Comparisons of oocyte maturation and of three methods of sperm preparation for their effects on the production of goat embryos in vitro. Theriogenology. 56, 503-516.
SAS. (1998). User’s guide. SAS Institute Inc. Version 6.12. Cary. NC. USA.
Silva M.A.C., Carnevale E.M., MacLellan L.J., Preis K.A., Leao K.M. and Squires E.L. (2002). Use of fresh, cooled and frozen semen during gamete intrafallopian transfer in mares. Theriogenology. 58, 763-766.
Wang Z.G., Xu Z.R. and Yu S.D. (2007). Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat. Czech J. Anim. Sci. 52, 21-25.
Wani N.A., Wani G.M., Khan M.Z. and Salahudin S. (2000). Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep. Small Rumi. Res. 36, 63-67.
Webb R., Campbell B.K., Garveric H.A. and Gong J.G. (1999). Molecular mechanisms regulating follicular recruitment and selection. J. Reprod. Fert. 45, 123-126.