1Department of Animal Science, Federal University, Dutse P.M.B. 7156, Jigawa State, Nigeria
2Department of Animal Science, University of Maiduguri, P.M.B. 1056, Borno State, Maiduguri, Nigeria
3Department of Animal Science, Kano University of Science and Technology, Wudil, P.M.B. 3244, Kano State, Nigeria
Receive Date: 26 July 2013,
Revise Date: 01 October 2013,
Accept Date: 15 October 2013
This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II stage was 61.41 ± 1.97%. The matured COCs were fertilized for 5 hours in Brackett and Oliphant media using fresh and frozen thawed semen separately. After fertilization the oocytes were cultured in TCM-199 for 48 hours to observe the cleavage rate. The maturation, fertilization and culture were performed in an incubator at 38.5 ˚C with 5% CO2 in humidified air. After fertilization cleavage rates were observed to check the fitness of zygotes to be morula and blastocyst. It was observed that the rates of normal fertilization (2 PN formations) for fresh and frozen semen were 36.02 ± 2.79 and 34.73 ± 2.58%, respectively and the cleavage rates were 25.19 ± 2.5 and 21.01 ± 2.8%, respectively. No significant differences (P>0.05) was observed between fresh and frozen semen in the efficiency of in vitro fertilization and subsequent development of goat embryos. It can be concluded that, both fresh and frozen semen can be used for IVF and subsequent development of goat embryos.
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