Polymorphism of DGAT1 Gene and Its Relationship with Carcass Weight and Dressing Percentage in Moghani Sheep Breed

Document Type: Research Article


Department of Animal Science, University of Tabriz, Tabriz, Iran


The diacylglycerol acyltransferase 1 gene (DGAT1) was identified as a strong candidate gene affecting mutton quality traits in sheep. Single nucleotide polymorphism creates a single base mutation (C to T) in AGCT site of endonuclease AluI. DGAT1 is one of the candidate genes to improve carcass characteristics in feedlot animals. In order to study area T487C in exon 17 of the DGAT1 polymorphism, Iranian Moghani sheep breeds randomly slaughtered in the abattoir were recorded. DNA was extracted from 150 samples of Moghani sheep. Polymerase chain reaction to amplify 309 bp of exon 17 DGAT1 gene using a pair of specific primers was performed. Genotypes obtained from method PCR-RFLP and directly from agarose gel. Two alleles T and C with frequencies of 0.829 and 0.171 were observed respectively. Statistical analysis showed polymorphism in exon 17 region of the gene significantly correlated with carcass weight and dressing percentage (P<0.05). So that the CC genotypes of the significant mean carcass weight and dressing percentage heavier than had TT genotypes (P<0.05). Of polymorphism can be observed that improvement in breeding programs to improve carcass weight and dressing percentage through selection in favor of superior genotypes be used.


Barillet F., Arranz J.J. and Carta A. (2005). Mapping quantitative trait loci for milk production andgenetic polymorphisms of milk proteins in dairy sheep. Genet. Sel. Eval. 37, 109-123.
Buhman K.K., Smith S.J., Stone S.J., Repa J.J. and Wong J.S. (2002). DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis. J. Biol. Chem. 277, 25477-25479.
Cases S., Smith S.J., Zhang Y.W., Meyers H.M., Lear S.R.E., Novak S.S., Welch C.C., Lusis A.J. and Erickson S.K. (1998). Identification of a gene encoding an acyl CoA: diacylglycerolacyltransferase, a key enzyme in triacyl glycerol synthesis. Proc. Natio. Acad. Sci. USA. 95, 13018-13023.
Chen H.C. and Farese R.V. (2000). DGAT and triglyceride synthesis: a new target for obesity treatment?. Trend. Cardio. Med. 10, 188-192.
Chen H.C., Jensen D.R., Myers H.M., Eckel R.H. and Farese R.V. (2002 ). Increased insulin and leptin sensitivity in mice lacking acyl CoA: diacylglycerol acyltransferase1. J. Clin. Invest. 109, 1049-1055.
Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni M., Reid S., Simon P., Spelman R., Georges M. and Snell R. (2002). Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yeild and composition. Genom. Res. 12, 222-231.
Meuwissen T.H.E., Hayes B.J. and Goddard M.E. (2001). Prediction of total genetic value usinggenome-wide marker maps. Genetics. 157, 1819-1829.
Mohammadi H., Shahrebabak M.M. and Sadeghi M. (2011). Study of polymorphism (T487C) exon 17 DGAT1 gene and carcass traits in two Iranian sheep breeds. J. Agric. Biotechnol. 1, 77-89.
Moioli B., Andrea M.D. and Pilla F. (2007). Candidate genes affecting sheep and goat milk quality. Small Rumin. Res. 68, 179-192.
Pannier L., Mullen A.M., Hamill R.M., Stapleton P.C. and Sweeney T. (2010). Association analysis ofsingle nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscularfat incrossbred Bos Taurus cattle. Meat Scie. 85, 515-518.
SASInstitute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC.
Schennink A., Stoop W.M., Visker M.H., Heck J.M., Bovenhuis H., van der Poel J.J., van Valenberg H.J. and van Arendonk J.A. (2007). DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Anim. Genet. 38, 467-473.
Souza F.R.P., Mercadante M.E.Z., Fonseca L.F.S., Ferreira L.M. S., Regatieri I.C., Ayres D.R., Tonhati H., Silva S.L., Razook A.G. and Albuquerque L.G. (2010). Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and theirrelationship with growth and carcass traits. J. Anim. Scie. 88, 435-441.
Winter A., Krämer W., Werner F.A.O., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G. and Fries R. (2002). Association of a lysine-232 / alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natio. Acad. Sci. USA. 14, 9300-9305.
Xu Q.L., Chen Y.L., Ma R.X. and Xue P. (2009). Polymorphism of DGAT1 associated with intramuscular fat-mediated tenderness in sheep. J. Scie. Food Agric. 89, 232-237.