Investigation of Kappa Casein Gene Polymorphism by PCR-RFLP in Najdi Cattle Breed Population in Khuzestan Province

Document Type: Research Article

Authors

Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran

Abstract

More than 80% of the total milk protein contains caseins that are to forms α-s1, α-s2, β-casein and kappa casein. Kappa casein is smaller than other milk proteins but due to have a role in size and stability of micelles. The present study describes polymorphism of kappa casein gene. This is the first study of kappa casein gene polymorphism in Najdi cattle of Iran. We used the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique to screen DNA polymorphism in cattle. We amplified the 453 fragment consisting on part of exon 4. The amplified fragment was digested with HinfI restriction endonuclease and subjected to electrophoretic separation in 3% agarose gel. Two alleles were observed, A and B. It gives frequencies of 0.54 and 0.46 for A and B alleles. Also, genotypes AA, AB and BB with frequencies of 35%, 37.5% and 27.5% of the total study population were diagnosed. Existence of Hardy-Weinberg equilibrium indicated that there was not any choice in order to increase or decrease the frequency of genotypes in the kappa-casein population in this herd. Also, in this population kappa-casein gene has medium level of heterozygosity.

Keywords


Alexander L.J., Stewart A.F., Mackinlay A.G. and Kapelinskaya T.V. (1988). Isolation and characterization of the bovine kappa-casein gene. European J. Biochem. 178, 395-401.
Azevodo A.L.S., Nassimento C.S., Steinberg R.S., Carvalho M.R.S., Peixoto M.G.G.D., Teodoro R.L., Guuimaraes S.E.F. and Machado M.A. (2008). Genetic polymorphism of the kappa casein gene in Brazilian cattle. Genet. Mol. Res. 7(3), 623-630.
Boettcher P.J., Tixier-Boichard M., Toro M.A., Simianer H., Eding H., Gandini G., Joost S., Garcia D., Colli L. and Ajmone-Marsan P. (2010). Objectives, criteria and methods for using molecular genetic data inpriority setting for conservation of animal genetic resources. J. Anim. Genet. 41(1), 64-77.
Brka M., Hodzic A., Reinsch N., Zecevic E., Dokso A., Djedovic R., Rukavina D., Kapur L., Vegara M., Sabanovic M. and Ravic I. (2010). Polymorphism of the kappa-casein gene in two Bosnian autochthonous cattle breeds. Arch. Tierz. 53, 277-282.
Dromuller C., Hamann H. and Distl O. (2001). Candidate gene markers for litter size in different German pig lines. J. Anim. Sci. 79, 2565-2570.
Dromuller C., Hamann H. and Distl O. (2001). Candidate gene markers for litter size in different German pig lines. J. Anim. Sci. 79, 2565-2570.
Farrell H.M., Jimenez-Falores R., Bleck R., Brown G.T. and Butler E.M. (2004). Nomenclature of the proteins of cows milk-sixth revision. J. Dairy Sci. 87, 1614-1674.
Ferretti L., Leone P. and Sgaramella V. (1990). Long range restriction analysis of the bovine casein genes. Nucl. Acid Res.18, 6829-6833.
Galila A.S.E. and Darwish S.F. (2008). A PCR-RFLP assay to detect genetic variants of kappa-casein in cattle and buffalo. Arab J. Biotechnol. 11, 11-17.
Jann O.C., Prinzenberg F.M., Luikart G., Caroli A. and Erhardt G. (2004). High polymorphism in the kappa-casein (CSN3) gene from wild and domestic caprine species revealed by DNA sequencing. J. Dairy Res. 71, 188-195.
Komisarek J., Waskowicz K. and Dorynek Z. (2006). Analysis of the relationship between two single nucleotide polymorphisms of the butyrophilin (BTN1A1) gene and milk production traits in Jersey cattle. Anim. Sci. 1, 45-52.
Konovalova E.N., Seltcov V.I. and Zinoveva N.A. (2004). Polymorphism gene kappa casein and its effects on production traits cows Simmental breeds. Pp. 24-25 in Proc. 4th Conf., Prob. Biotechnol. Farm Anim. VIJ. Dobrovitsy. Russia.
Kumar D., Gupta N., Ahlawat S.P.S., Satyanarayana R., Sunder S. and Gupta S.C. (2006). Single strand confirmation polymorphism (SSCP) detection in exon I of the α-lactalbumin gene of Indian Jamunapri milk goats (Capra hircus). Genet. Mol. Biol. 29, 271-74.
Lara M.A.C., Gama L.T., Bufarah G., Sereno J.R.B., Celegato E.M.L. and de Abreu U.P. (2002). Genetic polymorphisms at the kappa-casein locus in Pantaneiro cattle. Arch. Zootech. 51, 99-105.
Lien S. and Rogne S. (1993). Bovine casein haplotypes: number, frequencies and applicability as genetic markers. Anim. Genet. 24, 373-376.
Shende T.C., Sawane M.P. and Pawar V.D. (2009). Genotyping of Andharpuri buffalo for k-casein using PCR-RFLP. Tamilnadu J. Vet. Anim. Sci. 5(5), 174-178.
Tinaev A.S. (2003). Effects of polymorphic milk protein genes on milk yield at heifer black-pied breed. All-Russian Research Institute of Animal Breeding. Moscow. Russia.
Wu X.L., Mac Neil M.D., De S. and Xiao Q.J. (2005). Evaluation of candidate gene effects for beef backfat via Bayesian model selection. Genet. 125, 103-113.