Effect of Dietary Organic Zinc and Prebiotic on Productive Performance and Immune Response of Growing Quails

Document Type : Research/Original Article


1 Department of Animal Production, National Research Centre, Dokki, Cairo, Egypt

2 Department of Poultry Production, Faculty of Agriculture, Ain Shams University, Cairo, Egypt


The aim of the present study was to investigate the possibility of improvingproductive performance and immunity of growing Japanese quailreared during summer in Egypt by supplementing the diets with organic zinc (Bioplex zinc) and prebiotic (mannan oligosaccharides). Diets were a basal diet or the basal diet supplemented with 20 mg Bioplex zinc (3.0 mg Zn) (Zn1), 40 mg Bioplex zinc (6.0 mg Zn) (Zn2), 1.0 g prebiotic (P), P + Zn1 or P + Zn2. Ambient temperature and relative humidity ranged between 34 to 36 ˚C and between 45 to 51%, respectively. No significant difference was observed in body weight, body weight gain, feed intake, feed conversion and mortality of the treated quails when compared to the control group. The effect of treatments on the carcass traits and relative weights of the lymphoid organs and thyroid gland was not significant. The primary and secondary immune responses were the highest in the treated quails. The results showed that plasma total protein of the (P) and (P+Zn2) treated quails increased significantly when compared to the control group. The level of plasma total lipids and cholesterol decreased significantly (P<0.05) in quails fed the supplemented diets. No significant change was observed in the plasma AST and ALT enzyme activities and triiodothyronine concentration. It is concluded that, supplementing diets of growing Japanese quailsreared during summer in Egypt with 20 or 40 mg Bioplex zinc/kg each alone or in combination with 1.0 g/kg prebiotic (mannan oligosaccharides) had no significant effect onproductive performancebutimproved  their immune response.


Abou EL-Wafa S., Sayed M.A.M., Ali S.A. and Abdallah A.G. (2003). Performance and immune response of broiler chicks as affected bymethionine and zinc or commercial zinc-methionine supplementations.Egypt. Poult. Sci. 23, 523-540.
Akiba Y., Jensen L.S., Bart C.R. and Kraeling R.R. (1982). Plasma estradiol, thyroid hormones and liver lipids determination in birds. J. Nutr. 112, 299-308.
Al-Kassi G.A.M. and Witwit N.M. (2010). A comparative study on diet supplementation with a mixture of herbal plants and dandelion as a source of prebiotics on the performance of broilers. Pakistan J. Nutr. 9, 67-71.
Beisel W.R. (1982). Single nutrients and immunity. Am. J. Clin. Nutr. 35, 442-451.
Bonos E.M., Christaki E.V. and Florou-Paneri P.C. (2010). Effect of dietary supplementation of mannan oligosaccharides and acidifier calcium propionate on the performance and carcass quality of Japanese quail (Coturnix japonica). Int. J. Poult. Sci. 9, 264-272.
Bozkurt M., Küçükyilmaz K., Çatli A.U. and Çinar M. (2008). Growth performance and slaughter characteristics of broiler chickens fed with antibiotic, mannan oligosaccharide and dextran oligosaccharide supplemented diets. Int. J. Poult. Sci. 7, 969-977.
Bulbul T. and Küçükersan S. (2004). Yumurta tavugu rasyonlarina organik ve inorganic çinko katılmasının yumurta verimi ve kalitesi ile bazi kan parametreleri üzerineetkisi. Vet. Bilimleri. Dergisi. 20, 53-60.
Burns R.B. (1983). Antibody production suppressed in the domestic fowl by zinc deficiency. Avian Pathol. 12, 140-146.
Cao J., Henry P.R. and Guo R. (2000). Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants. J. Anim. Sci. 78, 2039-2054.
Defra. (2005). Heat stress in poultry solving the problem. http://www.defra.gov.uk. htm. Accessed Mar. 2005.
Dumas B.T. and Biggs H.G. (1972). Standard Methods of Clinical Chemistry. Academic press, New York, USA.
Duncan D.B. (1955). Multiple range and multiple F-test. Biometrics. 11, 1-42.
El-Kaiaty A.M., Ragab F.A. and Riad S.A. (2001). The effect of dietary zinc and / or methionine on some productive and immunological responses in two strains of chicks.Egypt. Poult. Sci. 21, 441-464.
Ensminger M.E., Oldfield J.E. and Heinemann W. (1990). Feeds and Nutrition. Ensminger Publishing Company, USA.
Feng J., Ma W.Q., Niu H.H., Wu X.M., Wang Y. and Feng J. (2010). Effects of zinc glycine chelate on growth, hematological and immunological characteristics in broilers. Biol. Trace. Elem. Res. 133, 203-211.
Ferket P.R. (2004). Alternatives to antibiotics in poultry production: response, practical experience and recommendations. Pp 57-66in Proc. 20th Alltech’s Annu. Symp. Nottingham Univ. Press, UK.
Geraert P.A., Padilha J.C.F. and Guillaumin S. (1996). Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: growth performance, body composition and energy retention. Brit. J. Nutr. 75, 195-204.
Gibson G.R. and Roberfroid M.B. (1995). Dietary modulation of the human colonic microbiota. Introducing the concept of prebiotics. J. Nutr. 125, 1401-1412.
Henry R.J. (1964). Clinical Chemistry: Principles and Techniques. Harper and Raw, New York, USA.
Hudson B.P., Fairchild B.D. and Wilson J.L. (2004). Breeder age and zinc source in broiler breeder hen diets on progeny characteristics at hatching. J. Appl. Poult. Res. 13, 55-64.
Ibs K.H. and Rink L. (2003). Zinc alter immune function. J. Nutr. 133,1452-1456.
Kaya S., Kececi T. and Haliloglu S. (2001). Effects of zinc and vitamin A supplements on plasma levels of thyroid hormones, cholesterol, glucose and egg yolk cholesterol of laying hens. Res. J. Vet. Sci. 71, 135-139.
Khalaji S., Zaghari M. and Nezafati S. (2011). The effects of manan-oligosaccharides on cecal microbial populations, blood parameters, immune response and performance of broiler chicks under controlled condition. African J. Biochem. Res. 5, 160-164.
Kidd M.T., Qureshi M.A., Ferket P.R. and Thomas L.N. (1994). Dietary zinc-methionine enhances mononuclear-phagocytic function in young turkeys. Biol. Trace. Elem. Res. 42, 217-229.
Klaver F.A.M. and Van Der Meer R. (1993). The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity. Appl. Environ. Microbiol. 59, 1120-1124.
Knight J.A., Anderson S. and Rawle J.M. (1972). Chemical base of the sulfo-phospho-vanilin reaction for estimation total serum lipids. Clin. Chem. 18, 199.
Mashaly M.M., Hendricks G.L., Kalama M.A., Gehad A.E., Abbas A.O. and Pattersont P.H. (2004). Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83, 889-894.
McDowell L.R. (2003). Minerals in Animal and Human Nutrition. Elsev. Sci., Amsterdam, the Netherlands.
Mohanna C. and Nys Y. (1999). Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens. Brit. Poult. Sci. 40, 108-114.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Research Council, National Academy Press, Washington, DC., USA.
Osman A.M.R. and Ragab M.S. (2007). Performance and carcass characteristics of broiler chicks fed diets supplemented with commercial zinc-methionine. Pp. 347-365 in Proc. 4th World Poult. Conf. Sharm El-Sheikh, Egypt.
Pelicano E.R.L., Souza P.A., Souza H.B.A., Leone l.F.R., Zeola N.M.B.L. and Boiago M.M. (2004). Productive traits of broiler chickens fed diets containing different growth promoters. Brazilian J. Poult. Sci. 6, 177-182.
Puvadolpirod S. and Thaxton J.P. (2000). Model of physiological stress in chickens. Response parameters. Poult. Sci. 79, 363-369.
Rahman M.M., Wahed M.A., Fchs G.J., Baqui A.H. and Alvarez J.O. (2002). Synergetic effect of zinc and vitamin A on the biochemical index of vitamin A nutrition in children. Am. J. Clin. Nutr. 1, 92-98.
Reitman S. and Frankel S. (1957). Calorimetric determination of AST and ALT activity. Am. J. Clin. Path. 28, 56-63.
Riad S.A., Safaa H.M., Fatma R. Mohamed Salwa S.S. and El-Minshawy H.A. (2010). Influence of probiotic, prebiotic and / or yeast supplementation in broiler diets on the productivity, immune response and slaughter traits. J. Anim. Poult. Prod. 1, 45-60.
Richmond W. (1973). Preparation and properties of bacterial cholesterol oxidase from Nocardia and its application to the enzymatic assay of total cholesterol in serum. Clin. Chem. 19, 1350-1356.
Rossi P., Rutz F., Anciuti M.A., Rech J.L. and Zauk N.H.F. (2007). Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. J. Appl. Poult. Res. 16, 219-225.
Sarica S., Corduk M., Yarim G.F., Yenisehirli G. and Karatas U. (2009). Effects of novel feed additives in wheat based diets on performance, carcass and intestinal tract characteristics of quail. South African J. Anim. Sci. 39, 144-157.
SAS. (1994). SAS/STAT®User's Guide: Statistics Ver. 6.04, 4th ed. SAS Institute Inc., Cary, NC.
Shashidhara R.G. and Devegowda G. (2003). Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity. Poult. Sci. 82, 1319-1325.
Silva V.K., Della Torre da Silva J., Torres K.A.A., de Faria Filho D.E., Hirota Hada F. and Barbosa de Moraes V.M. (2009). Humoral immune response of broilers fed diets containing yeast extract and prebiotics in the prestarter phase and raised at different temperatures. J. Appl. Poult. Res. 18, 530-540.
Spears J.W., Grimes J., Lloyd K. and Ward T.L. (2003). Efficacy of a novel organic selenium compound (zinc-l-selenomethionine, Availa Se) in broiler chicks. Pp. 197-198 in Proc. 1st Latin Am. Cong. Anim. Nutr., Cancun, Maxico.
Taherpour K., Moravej H., Shivazad M., Adibmoradi M. and Yakhchali B. (2009). Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. African J. Biotechnol. 8, 2329-2334.
Tizard I.R., Carpenter R.H., McAnalley B.H. and Kemp M.C. (1989). The biological activities of mannans and related` complex carbohydrates. Mol. Biother. 1, 290-296.
Uyanik F., Eren M., Atasever A., Tunçoku G. and Kolsuz A.H. (2001). Changes in some biochemical parameters and organs of broilers exposed to cadmium and effect of zinc on cadmium induced alterations. Israel J. Vet. Med. 56, 128-134.
Van Der Zijpp A.J., Frankena K., Boneschauscher J. and Nieuwland M.G.B. (1983). Genetic analysis of primary and secondary immune responses in the chicken. Poult. Sci. 62, 565-572.
Vytautas S., BobinieneR., GudaviciuteD., cepulieneR., Semaška V., Vencius D. and KepalieneI. (2006). Influence of a prebiotic feed additive on some biochemical indices of blood and intestinal microbiota of broiler chickens. Lietuvos. Mokslu. Akademija. 4, 57-62.
Waldroup P.W., Fritts C.A. and Yan F. (2003). Utilization of Bio-Mos® mannan oligosaccharide and Bioplex® copper in broiler diets. Int. J. Poult. Sci. 2, 44-52.
Yalçinkaya H., Gungori T., Bafialani M. and Erdem E. (2008). Mannan oligosaccharides (MOS) from Saccharomyces cerevisiae in broilers: effects on performance and blood biochemistry. Turk. J. Vet. Anim. Sci. 32, 43-48.
Yalçinkaya I., Çınar M., Yildirim E., Erat S., Basalan M. and Güngör T. (2012). The effect of prebiotic and organic zinc alone and in combination in broiler diets on the performance and some blood parameters. Italian J. Anim. Sci. 11, 298-301.
Yang Y., Iji P.A., Kochert A., Mikkelsen L.L. and Choct M. (2007). Effects of mannanoligosaccharide on growth performance, the development of gut microflora and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res. 16, 280-288.
Volume 3, Issue 4 - Serial Number 4
December 2013
Pages 761-767
  • Receive Date: 25 September 2012
  • Revise Date: 24 December 2012
  • Accept Date: 31 December 2012
  • First Publish Date: 01 December 2013