The Effect of Cinnamon Extract on Spermatogenesis Hormonal Axis of Pituitary Gonad in Mice

Document Type : Research Articles


1 Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran

2 Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran

3 Department of Biology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran


Cinnamon has many therapeutic effects, such as its impact the increase of sexual ability. This experiment was conducted to determine the effect of cinnamon extract on spermatogenesis and hormonal axis of pituitary gonad in mice. The animals used in this study are male adult mice (weighing about 30-34 g and 9-10 weeks old). Cinnamon was purchased from one of the most valid shops in Jahrom and then powdered. After preparation of cinnamon extract, it was used for injection. The concentration of cinnamon used in this research was 10 and 20 mg/kg BW. The control animals received only drinking water. The sham group received distilled water. The treatment animals received cinnamon extract (10 and 20 mg/kg BW) for two weeks, each day for the amount of 1 mL interaperitoneally. After the injection, the animals were sacrificed. The weights of testes were measursed, and the changes, including the number of Sertoli cells, spermatogonia, spermatid, and Leydig cells were calculated. Also, hormonal concentration changes (LH, FSH and Testosterone) were measured by special hormonal kits. The results revealed significant increase (P<0.05) in the number of spermatogonia, spermatocyte, spermatid, Sertoli and Leydig cells in treated animals. Also, the results showed significant increase (P<0.05) in the concentration of LH, FSH and Testosterone hormones among treated animals. Due to the increase in LH, FSH and Testosterone hormones and also the increase of the number of Leydig cells, it is concluded that cinnamon may cause an increase in sex cells within seminiferous tubules in mice.


AndersonR.A., Broadburst C.L., Polansky M.M., Schmidt W.F., Khan A. and Flanagan V.P. (2004). The effect of cinnamon on diabetes in adult male rats. Isolation Food Chem. 14, 52-70.
Bernard A.J., Damber E. and Widmark  A. (2005). Hormonal control of testicular blood flow. Mol. Cell. Endocrinol. 50, 123-133.
Kamath J.V., Rana A.C. and Chowdhury A.R. (2003). Pro healingeffect of cinnamon zeylanicum bark.Phytother Res.18, 970-972.
Kander B.G. and Hsueh A.J. (2009). Intragonadal hormonoes in rats. Endocrinology.118, 23-310.
Keys, A. and Anderson, T. (1995). Metabolism. 14, 747-775.
Khan A., Sfdar M., Ali Khan M.M., Khattak K.N. and Anderson R.A. (2003). Cinnamon improves glucose and lipids of people with type 2 diabetes. Diabetes Car. 26, 3215-3218.
Kosior K.U. and Bobowiec R. (2006). Leptin effect on nitric oxide and GnRH-induced FSH secretion from ovine pituitary cells in vitro. J. Physiol. Pharmacol. 57, 637-647.
Lee W., Mason A.R. and Mather J.P. (2004). Interestitial cell cultures secrete and activity with characteristics of the hormones. Mol. Cell.Endocrinol. 50, 21-27.
Lindly Did V.L. and Miler J. (1984). Cembridge Elemantary Statistical Table (CEST). CembridgeUniversity.
Nir Y., PotasmanI., Stermer E., Tabak M. and NeemanI.(2000). Controlled trial of the effect of cinnamon extract on Helicobacter pylori. Helicobater. 5, 94-97.
NormanJ.F. and Way J.M. (1998). Some ecological observations in hypothalamic hormones. Endocrinolology. 2, 532-548.
Parivzi N. and Ellendorff F. (1982). Further evidence on dual effects of norepinphrine on LH Secretion. Neuro Endocrinol. 35, 48-55.
Parvinen M.K., Vinko K. and Topari J. (2007). Cell interaction during the seminiferous epithelial cycle. Cytology. 104, 114-129.
Sato Y. and Tsukanmamoto T. (2000). Effects of nitric oxide stimulation on the brain. Drugs Today. 36, 38.
Shagauo R.B. and Davidson A.M. (2006). The effect of Cinnamomum zeylanicum on histological structure of testis in rats. Endocrinology.63, 241-252.
Sharp R.M. (2009). Interatesticular factors of controlling of testicular function. Biol.  Rep.30, 29-49.
Shing G., Maurya S., Deamopna M.P., Catanlan C.A.and Cesar A.N. (2007). A comparision of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oil. Food Chem. Toxicol.45, 1650-1661.
Skidmore R.L. (2002). Handbook of Herbs and Netura Supplemnts, 2th Ed. St Louis, Mosey, 38.
Vinkovsky G.J., Canilleau J. and Billuau A. (2003). Interleukin- 1 and Leydig cells. Mol. Cell. Endocrinol. 57, 51-60.
Xia Z.F. and Richard R.A. (2006). The brain-Pituitary adipocyte axis role of leptin in modulating neuroendocrine function. J. Anim. Sci.77, 12349-12357.