Effect of Phosphorus Supplementation to Ammoniated Rice Straw on Rumen Fermentability, Microbial Protein Synthesis and in vitro Nutrient Degradability

Document Type: Research Articles

Authors

1 Departement of Animal Production, Faculty of Agriculture, Muaro Bungo University, Pendidikan Street Sungai Binjai km 6.5 Muaro Bungo Jambi, Indonesia

2 Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, 25163, Indonesia

3 Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Kampus Dramaga, Bogor, 16680, Indonesia

Abstract

The effect of phosphorus supplementation to ammoniated rice straw was studied. The in vitro experiment was carried out following the first stage of Tilley and Terry method. The treatments consisting of four diets were A= 50% ammoniated rice straw + 50% concentrate (control), B= A + 0.2% phosphorus (P) supplement, C= A + 0.4% phosphorus (P) supplement, and D= A + 0.6% phosphorus (P) supplement of dry matter. Completely randomized design was used as the experimental design with differences among treatment means were examined using Duncan’s multiple range test. Variables measured were total bacterial and cellulolytic bacterial population, cellulolytic enzyme activity, ammonia (NH3) and volatile fatty acid (VFA) concentrations, as fermentability indicators and synthesized microbial protein, as well as degradability indicators including dry matter (DM), organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and cellulose. The results indicated that fermentability and degradability of diets consisting ammoniated rice straw with P supplementation were significantly higher than the control diet (P<0.05). It is showed that P supplementation is important for rumen fermentation and growth of rumen microbes. Overall supplementation of phosphorus at 0.4% of dry matter to ammoniated rice straw shown best results in terms of rumen fermentation, microbial protein synthesis and in vitro degradability.

Keywords


AOAC. (1990). Official Methods of Analysis. Vol.I. 15th Ed., Association of Official Analytical Chemists, Arlington, Virginia.
Bravo D., Sanvant D., Bogaert C. and Meschy F. (2003). Quantitative aspect of phosphorus absorption in ruminant. Reprod. Nutr. Dev. 43, 271-284.
ChandlerP.T. (1996). Environmental challenges as related to animal agriculture-dairy. Pp. 7-9 in Nutrient Management of Food Animals to Enhance and Protect the Environment. E.T. Kornegay, Ed., CDR Lewis Publ, New York.
Durand M. and Kawashima R. (1980). Influence of minerals in rumen microbial digestion. Pp. 375-408 inDigestive Physiology and Metabolism in Ruminants. Ruckebush and Thivend Ed., MTP Press, Ltd., Lancaster, England.
Goering H.K. and Van Soest P.J. (1970). Forage Fiber Analysis (apparatus, reagents, procedure and some applications). Pp. 1-20 inAgricultural Handbook. United States Department of Agricultural Ed., US Government Printing Office, Washinggton D.C.
Gopar A.H. (1981). Pengaruh perubahan laju penggantian isi rumen terhadap kegiatan metabolisme mikrobanya dalam kondisi in vitro. Skripsi. Fakultas Peternakan. Institut Pertanian Bogor.
Knowlton K.F. and Herbein J.H. (2002). Phosphorus partitioning during early lactation in dairy cows fed diets varying in phosphorus contents. J. Dairy Sci. 85(5), 1227-1236.
Komisarczuk S. and Durand M. (1991). Effects of minerals on microbial metabolism. Pp. 179-198 inRumen Microbial Metabolism and Ruminant Digestion. J.P. Jouany Institut National de la Recherche Agronomique (INRA), Paris.
Komisarczuk S., Merry R.J. and McAllan A.B. (1987). Effect of different levels of phosphorus on rumen microbial fermentation and synthesis determined using a continuous culture technique. British J. Nutr. 57, 279- 290.
MiltonJ.T.B. and Ternouth J.H. (1984). The effects of phosphorus upon in vitro microbial digestion. Anim. Prod. Australia. 15, 472- 475.
PettersonJ. (2002). Defining the phosphorus requirements of cattle. Pp. 11-19 inProc. 37th Pac. NW. Anim. Nutr. Conf.
Steel R.G.D. and Torrie J.H. (1980). Principles and Procedure of Statistics. McGraw-Hill Book Co. Inc. New York.
Suryahadi. (1990). Penuntun Praktikum Ilmu Nutrisi Ruminansia. Pusat Antar Universitas Ilmu Hayat. IPB. Bogor.
Tilley J.M. and Terry R.A. (1969). A two stage technique for in vitro digestion of forage crops. J. British Grass. Soci. 18(2), Pp. 104-111.
Valk H. and Sebek L.B.J. (1999). Influence of long-term feeding of limited amounts of phosphorus on dry matter intake, milk production and body weight of dairy cows. J. Dairy Sci. 82, 2157-2163.
Van Soest P.J. (2006). Rice straw the role of silica and treatment to improve quality. J. Anim. Feed Sci. and Technol. Vol. 130, Pp. 137-171.
Weiss W.P. and Wyatt D.J. (2004). Macromineral digestion by lactating dairy cows: Estimating phosphorus excretion via manure. J. Dairy Sci. 87(7), 2158-2166.
Widyastuti A.T. (2005). Isolasi Dan Uji Kemampuan Bakteri Simbion Rayap Pendegradasi Serat. Skripsi. Fakultas Peternakan. Institut Pertanian Bogor.
Wu Z., Tallam S.K., Ishler V.A. and Archibald D.D. (2003). Utilization of phosphorus in lactating cows fed varying amounts of phosphorus and forage. J. Dairy Sci. 86, 3300-3308.