1Department of Animal Production, National University of Rwanda, University Avenue, Butare, Rwanda
2Department of Pure and Applied Chemistry, National University of Rwanda, University Avenue, Butare, Rwanda
Receive Date: 11 September 2013,
Revise Date: 20 October 2013,
Accept Date: 30 October 2013
An eight week study was conducted to investigate the effect of inclusion of tomato waste meal in layer diets on laying performance, egg quality and lipid profile as a means to reduce feed cost and pollution. Four laying diets containing tomato waste meal (TWM) at four levels of 0, 3, 6 or 9% were assigned randomly to 4 groups of 40 birds divided among four replicates of 10 birds in each dietary treatment in a completely randomized experimental design. Collected on egg production, egg quality, yolk lipid profile and its carotenoid content which were analyzed byanalysis of variance using the general linear model procedure of SAS. Statistical results showed no differences in hen-day production at either 0, 3 or 6% inclusion level (68.30%, 67.80% and 68.10% respectively) but at the 9% (63.45% hen-day production) of inclusion level a significant (P<0.05) decrease was observed. Feed conversion ratio was significantly higher at the 9% inclusion level. Egg weight, Haugh unit and yolk index were not affected by dietary treatments. Yolk colour increased significantly with increasing levels of TWM in the diets. Yolk lipids (including cholesterol) were at the lowest amounts at 6% and 9% inclusion level. Yolk carotenoid content (including lycopene) of the birds that were fed diets containing TWM was significantly increased with increasing dietary content of TWM. It was concluded that up to 6% TWM can be included in layer diets without any adverse effect on egg quality and compromising egg production rate. This inclusion level also showed to be beneficial via enhancing yolk colour score and lycopene concentration and reducing egg yolk cholesterol content.
A.O.A.C. (1990). Official methods of analysis 15th ed. Association of Official Analytical Chemist, Washington D.C. Agah M.J., Nassiri-Moghaddam H., Tahmasbi A.M. and Lotfollahian H. (2010). Performance and fatty acid composition of yolk lipid from laying hens fed with locally produced canola seed (Brassica napus). Res. J. Biol. Sci. 5, 228-232. Agarwal A., Shen H., Agarwal S. and Rao A.V. ( 2001). Lycopene content of tomato products: its stability, bioavailability and in vivo antioxidant properties. J. Med. Food. 4, 9-15. Alvarado A., Pacheco-Delahaye E. and Hevia P. (2001). Value of a tomato by-product as a source of dietary fiber in rat. Plant Foods Hum. Nutr. 56, 335-348. Barber N.J. and Barber J. (2002). Lycopene and prostate cancer. Prost.Cancer Prost. Dis.5,6-12. FAOSTATA (2011). AFood and Agriculture Organization of the United Nations. Tomato production quantity. from: http://faostat3.fao.org/home/index.html. Accessed Sep. 2013. Giovannucci E., Rimm E.B., Liu Y., Stampfer M.J. and Willett W.C. (2002). A prospective study of tomato byproducts, lycopene and prostate cancer risk. J. Natl. Cancer Inst. 94, 391-398. Hart D.J. and Scott K.J. (1995). Development and evaluation of HPLC method for the analysis of carotenoids in foods and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chem. 54, 101-111. Haugh R.R. (1937). The Haugh unit for measuring egg quality. US Egg. Poult. Mag.43, 522-555. Ijarotimi O.S. and Olopade A.J. (2009). Determination of amino acid content and protein quality of complementary food produced from locally available food materials in Ondo State, Nigeria. Mal. J. Nutr.15, 87-95. Jenkins D., Wolever T., Rao A.V., Hegele R.A., Mitchell S.J., Ransom T., Boctor D.L., Spadafora P.J., Jenkins A.L., Mehling C., Relle L.K., Connelly P.W., Story J.A., Furumoto E., Corey J. and Wursch P. (1993). Effect of blood lipids of very high intakes of fiber in diets low in saturated fat and cholesterol. N. Engl. J. Med. 329, 21-26. Kivini H., Jarvenpaa E.P., Aro H., Huopalahti R. and Ryhanen E.L. (2004). Qualitative and quantitative liquid chromatographic analysis methods for the determination of the effects of feed supplements on hen egg yolk phospholipids.J. Agric. Food Chem. 52, 4289-4295. Knoblich M., Anderson B. and Latshaw D. (2005). Analyses of tomato peel and seed by-products and their use as a source of carotenoids. J. Sci. Food Agric. 85,1166-1170. Li F., Zhang L.M., Wu X.H., Lic Y., Yang X.J., Dong Y., Lemme A., Han J.C. and Yao J.H. (2013). Effects of metabolizable energy and balanced protein on egg production, quality and components on Lohmann Brown laying hen. J. Appl. Poult. Res. 22, 36-46. Lira R.C., Robello C.B., Ludke M.M.M., Ferreira P.V., Lana G.R.Q. and Lana S.R.V. (2010). Productive performance of broiler chicken fed tomato waste. R. Brasilian Zootec. 39, 1074-1081. Liu L.Y., Yang M.H., Lin J.H. and Lee M.H. (2005). Lipid profile and oxidative stability of commercial egg products. J. Food Drug. Analysis.13, 78-83. McNaughton J.L. (1978). Effect of dietary fiber on yolk, liver and plasma cholesterol concentrations of the laying hen.J. Nutr. 108, 1842-1848. Monika K., Brandi A. and David L. (2005). Analyses of tomato peel and seed byproducts and their use as a source of carotenoids. J. Sci. Food Agric. 85, 1166-1170. Oladunjoye I.O. and Ojebiyi O.O. (2010). Performance characteristics of broiler chicken (Gallus gallus) fed rice (Oriza sativa) bran with or without Roxazyme G2G. Int. J. Anim. Vet. Adv. 2, 135-140. Pasin G., Smith G.M. and Mahony M.O. (1998). Rapid determination of total cholesterol in egg yolk using commercial diagnostic cholesterol reagent. Food Chem. 61, 255-259. Rezaei M. and Hafezian M. (2007). Use of different levels of high fibre sunflower meal in commercial leghorn type layer diets. Int. J. Poult. Sci.6, 431-433. Rehman A., Bourne L.C. and Halliwell B. (1999).Tomato consumption modulates DNA damage in humans. Biochem. Biophys. Res. Commun. 262, 828-831. Safamehr A., Malek H. and Nobakhat A. (2011). The effect of different levels of tomato pomace with without multi-enzyme on performance and egg traits of laying. Iranian J. Appl. Anim. Sci.1, 39-47. SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC.