Receive Date: 07 July 2011,
Revise Date: 20 October 2011,
Accept Date: 21 October 2011
The aim of the experiment was to determine the effect of different dry matter content and addition of silage inoculant on the ruminal protein degradability. Alfalfa herbage was collected from two cuts and the fresh matter was sampled two times during each cut (with different dry matter content). During the preparation of experimental micro-silages, the chopped forage was treated with a microbial inoculant or it was left untreated (control). The in sacco method was used for the measurement of ruminal protein degradability. The different dry matter content significantly (P<0.05) affected the rate of ruminal protein degradation in alfalfa silages. The average values of ruminal protein degradability of alfalfa silages were at lower dry matter content of 70.72±1.243%, at higher dry matter content 73.69±0.554% in the first cut. The ruminal protein degradability was significantly (P<0.01) different in the second cut. The alfalfa silages had average value of ruminal protein degradability at lower dry matter content of 69.19±1.184%, at higher dry matter content of 72.74±0.637%. The applied ensiling additive had no statistically significant influence on the ruminal protein degradability in alfalfa silages. In first cut, it was measured in control silages as 72.08±1.620% and in inoculated silages as 72.33±1.901%. In second cut, the average values of ruminal protein degradability of control silages were 71.56±1.833% and of inoculated silages as 70.37±2.014%. The using of bacterial inoculant had no significant effect of ruminal protein degradability, but improved the fermentation process quality.
AOAC. (1990). Association of Official Analytical Chemists. Herlick K. (Ed) 15th Ed. Arlington, USA, 1230 Pp. Aufrère J.,Graviou D., Baumont R., Detour A. and Demarquilly C. (2000). Degradation in the rumen of proteins from fresh lucerne forage in various stagesof growth and conserved as silage or hay. Animal Research. http://www.scopus.com. BroderickG.A. (1995). Desirable characteristics of forage legumes for improving protein utilization in ruminants. J. Anim. Sci. 73, 2760-2773. Grabber J.H. (2009). Forage management effects on protein and fiber fractions, protein degradability, and dry matter yield of red clover conserved as silage. Anim. Feed Sci. Technol.154, 284-291. Griffin, T.S., Cassida K.A., Hesterman O.B. and Rust S.R.(1994). Alfalfa maturity and cultivar effects on chemical and in situ estimates of protein degradability. Crop.Sci. 34, 1654-1661. Hawkins D.R., Henderson H.E. and Purser D.B. (1970). Effect of dry matter levels of alfalfa silage on intake and metabolism in the ruminant. J. Anim. Sci. 31, 617-625. Hristov A.N. and Sandev S.G. (1998). Proteolysis and rumen degradability of protein in alfalfa preserved as silage, wilted silage or hay. Anim. Feed Sci. Technol. 72,175-181. Huhtanen P. (2010). Evaluation of protein value of forages. Pp 58-76 in Proc. Forage Conservation. Mendel University Brno. Kacerovsky O. (1990). Zkoušení a posuzování krmiv. Monograph. Praha. Kohn R.A. and Allen M.S. (1995). Effect of plant maturity and preservation method on in vitro protein degradation of forages. J. Dairy Sci.78,1544-1551. Kowalski Z.M., Gorka P. and Pisulewski P.M. (2010). The rumen and intestinal degestibility of rumen by-pass amino acids. Pp. 92-96 in Proc. Forage Conservation.Mendel University Brno. Kramer E., Mayrhuber E. and Mathies E. (2010). Control of protein degradation due to clostridia. Pp. 150-154 in Proc. BOKUSYMPOSIUM TIERERNÄHRUNG.Wien: Institut für Tierernährung, Tierische Lebensmittel und Ernährungsphysiologie. Luchini N.D.,Broderick G.A., Muck R.E., Vetter R.L. and MakoniN.F.(1997). Effect of storage system and dry matter content on the composition of alfalfa silage. J. Dairy Sci.80, 1827-1832. Muck R.E. (1990). Dry matter level effects on alfalfa silage quality.II. Fermentation products and starch hydrolysis. Trans. American Soci. Agric. Eng.33,373-381. Mustafa A.F., Christensen D.A. and Mckinnon J.J. (2000). Effects of pea, barley, and alfalfa silage on ruminal nutrient degradability and performance of dairy cows. J. Dairy Sci.83,2859-2865. Peltekova V.D. and Broderick G.A. (1996). In vitroruminal degradation and synthesis of protein of fractions extracted from alfalfa hay and silage. J. Dairy Sci.79, 612-619. Petit H.V. and Flipot P.M. (1990). Intake, duodenal flow, and ruminal characteristics of long or short chopped alfalfa-timothy silagewith or without inoculant. J. Dairy Sci.73, 3165-3171. Richter M. and Trinacty J. (2009). Assessment of Proteins in Dairy Cow Fodders According to INRA System. Handbook.Šumperk. Rizk C., Mustafa A.F. and Phillip L.E. (2005) Effects of inoculation of high dry matter alfalfa silage on ensiling characteristics, ruminal nutrient degradability and dairy cow performance. J. Sci. Food Agric.85, 743 - 750. Ruggieri A.C., Tonani F.L., Andrade P. and Silveira A.C.(2001). Effect of wilting and addition of corn meal on in situ degradability of alfalfa silage (Medicago sativa L.). Arquivo brasiliero de medicina veterinaria e zootecnia.51, 94-99. Tyrolova Y. and Vyborna A. (2008). Effect of the stage of maturity on the leaf percentage of lucerne and the effect of additives on silage. Czech. J. Anim. Sci. 53, 330-335. Verbic J., Orskov E.R., Zqainar J., Chen X.B. and Znidarsic-Ponqrac V. (1999). The effect of method of forage preservation on the protein degradability and microbial protein synthesis in the rumen. Anim. Feed Sci. Technol. 82, 195-212. Wilkinson J.M. (2005). Silage. Monograph. Lincoln : Chalcombe Publications. Yu P., Kristensen D.A. and Mckinnon J.J. (2004). In situ rumen degradation kinetics of timothy and alfalfa as affected by cultivar and stage of maturity. Canadian J. Anim. Sci. 84, 255-263.