Ala Noshahr, F., Rafat, A. (2014). Genetic Polymorphism of GDF9 Gene in Iranian Moghani Sheep Breed. Iranian Journal of Applied Animal Science, 4(4), 887-890.
F. Ala Noshahr; A. Rafat. "Genetic Polymorphism of GDF9 Gene in Iranian Moghani Sheep Breed". Iranian Journal of Applied Animal Science, 4, 4, 2014, 887-890.
Ala Noshahr, F., Rafat, A. (2014). 'Genetic Polymorphism of GDF9 Gene in Iranian Moghani Sheep Breed', Iranian Journal of Applied Animal Science, 4(4), pp. 887-890.
Ala Noshahr, F., Rafat, A. Genetic Polymorphism of GDF9 Gene in Iranian Moghani Sheep Breed. Iranian Journal of Applied Animal Science, 2014; 4(4): 887-890.
Genetic Polymorphism of GDF9 Gene in Iranian Moghani Sheep Breed
Department of Animal Science, University of Tabriz, Tabriz, Iran
Receive Date: 07 November 2013,
Revise Date: 15 January 2014,
Accept Date: 30 January 2014
Abstract
The families of TGF-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. Three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (GDF9), BMP15 and BMPR-IB have been shown to be essential for follicular growth and ovulation. Different mutations in the GDF9 gene cause increased ovulation rate and infertility in a dosage-sensitive manner in sheep. In this study, blood samples were collected from 150 Moghani sheep breed using venojects treated with the anti-clot substance (EDTA) and subsequently their DNA content were salted out and extracted. The quantity and quality of extracted DNA was examined using spectrophotometery and gel electrophoresis, respectively. With using a pair of specific primers, the DNA fragment was amplified from exon 1 of GDF9 (462 bp). Digested PCR products with HhaI enzyme showed a G to A substiuation in GDF9 locus. The wild type allele of this gene (G/+) with two restriction site resulted DNA fragments of 156, 52 and 254 bp while the mutant allele (G/-) with one restriction site resulted two DNA fragments with the size of 52 and 410 bp. Genotype frequencies for G (+/+), G (+/-) and G (-/-) were 0.66, 0.24 and 0.1, respectively. From studied luci, GDF9 was polymorphic in Iranian Moghani sheep breed.
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