Molecular Study on the Exon 2 Region of the Ovis Bone Morphology Protein 15 (BMP-15) Gene in Iranian Bluchi Sheep Breed by PCR-SSCP Technique

Document Type: Research Article

Authors

1 Department of Animal Science, Technical and Vocational University, Tehran, Iran

2 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran

4 Department of Agriculture, Payame Noor University, Tehran, Iran

Abstract

Litter size is one of the most important economical traits in sheep breeding industry. In addition to minor genes, litter size is under the influence of major genes. Bone morphogenetic protein 15 (BMP-15), a member of the transforming growth factor beta (TGF-ß) superfamily, which is specifically expressed in oocytes, plays a dramatic role in sheep prolificacy. Reported mutations in this gene cause increased ovulation rate and infertility in a dosage-sensitive manner. Six different point mutations have been indicated in the BMP-15 gene of sheep, each having a major effect on prolificacy. The aim of the current study was to investigate the Lacaune (FecXL) mutation in the prolificacy of the Iranian Baluchi sheep breed. Blood samples were collected in ethylenediaminetetraacetic acid(EDTA) coated tubes from jugular vein and genomic DNA was extracted from whole blood samples. Single nucleotide polymorphism of FecXL loci in BMP-15 gene were determined using polymerase chain reactionsingle-strand conformation polymorphism(PCR-SSCP) technique. No evidence of mutation in FecXL was observed, all of which were monomorphic for exon 2 BMP-15 gene.

Keywords

References

  1. Bodin L., Di Pasquale E., Fabre S., Bontoux M., Monget P., Persani L. and Mulsant P. (2007). A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep.Endocrinology. 148, 393-400.
  2. Burland T.G. (2000). DNA STAR's Lasergene sequence analysis software.Methods Mol. Biol. 132, 71-91.
  3. Chena D., Ming Z. and Gregory R. (2004). Bone morphogenetic proteins. Grow. Fact. 22, 233-241.
  4. Davis G.H., McEwan J.C., Fennessy P.F., Dodds K.G. and Farquhar P.A. (1991). Evidence for the presence of a major gene influencing ovulation rate on the X-chromosome of sheep.Biol. Reprod. 44, 620-624.
  5. Davis G.H., Balakrishnan L., Ross I.K., Wilson T., Galloway S.M., Lumsden B.M., Hanrahan J.P., Mullen M., Mao X.Z., Wang G.L., Zhao Z.S., Zeng Y.Q., Robinson J.J., Mavrogenis A.P., Papachristoforou C., Peter C., Baumung R., Cardyn P., Boujenane I., Cockett N.E., Eythorsdottir E., Arranz J.J. and Notter D.R. (2006). Investigation of the Booroola (FecB) and Inverdale (FecX (I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries.Anim. Reprod. Sci. 92, 87-96.
  6. Deldar-Tajangookeh A., Zare Shahneh A., Zamiri M.J., Daliri M., Kohram H. and Nejati-Javaremi A. (2009). Study of BMP-15 gene polymorphism in Iranian goats. African J. Biotechnol. 8,2929-2932.
  7. Di Pasquale E., Beck-Peccoz P. and Persani L. (2004). Hypergonadotropic ovarian failure associated with an inherited mutation of human bone morphogenetic protein-15 (BMP15) gene. Am. J. Human Gen. 75, 106-111.
  8. Elvin J.A., Yan C. and Malsuk M.M. (2000). Oocytes-expressed TGF-beta superfamily members in female fertility. Mol. Cell. Endocrinol. 159,1-5.
  9. Galloway S.M., McNatty K.P., Cambridge L.M., Laitinen M.P.E., Juengel J.L., Jokiranta T.S., McLaren R.J., Luiro K., Dodds K.G. and Montgomery G.W. (2000). Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner.Nat. Genet. 25, 279-283.
  10. Gholibeikifard A., Aminafshar M. and Hosseinpour Mashhadi M. (2013). Polymorphism of IGF-I and ADRB3 genes and their association with growth traits in the Iranian Baluchi sheep. J. Agric. Sci. Technol. 15, 1153-1162.
  11. Hanrahan J.P., Gergan S.M., Mulsant P., Mullen M., Davis G.H., Powell R. and Galloway S.M. (2004). Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in cambridge and belclare sheep (Ovis aries). Biol. Reprod. 70, 9000-9009.
  12. Kareta W., Korman K. and Cegla M. (2006). Ovulation level and prolificacy in ewes depending on their age, birth type and percentage of prolific genotype. Reprod. Biol. 6, 73-78.
  13. Knight P.G. and Glister C. (2006). Focus on TGF-ßsignalling: TGF-ßsuperfamily members and ovarian follicle development. Reproduction. 132, 191-206.
  14. McNatty K.P., Smith P., Moore L.G., Reader K., Lun S., Hanrahan J.P., Groome N.P., Laitinen M., Ritvos O. and Juengel J.L. (2005). Oocyte expressed genes affecting ovulation rate. Mol. Cell. Endocrinology. 234, 57-66.
  15. Monteagudo L.V., Ponz R., Tejedor M.T., Laviña A. and Sierra I. (2009). A 17bp deletion in the bone morphogenetic protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed. Anim. Reprod. Sci. 110, 139-146.
  16. Montgomery G.W., Crawford A.M., Penty J.M., Dodds K.G., Ede A.J., Henry H.M., Pierson C.A., Lord E.A., Galloway S.M., Schmack A.E., Sise J.A., Swarbrick P.A., Hanrahan V., Buchanan F.C. and Hill D.F. (1993). The ovine Booroola fecundity gene (FecB) is linked to markers from a region of human chromosome 4q. Nat. Genet. 4, 410-414.
  17. Montgomery G.W., Galloway S.M., George H., Davis G.H. and McNatty K.P. (2001). Genes controlling ovulation rate in sheep. Reproduction. 121, 843-852.
  18. Nejati-Javaremi A., Rahimi G. and A.A. (2007). Detection of polymorphism in FecXL gene associated with twinning in Lori-Bakhtiari sheep breed using PCR-SSCP. Pp. 520 in Proc. 5th Natio. Biotechnol. Cong. Iran. Tehran, Iran.
  19. Souza C.J.L., MacDougall C., Campbell B.K., McNeilly A.S. and Baird D.T. (2001). The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene. J. Endocrinol. 169, 1-6.
  20. Zare Y., Nejati-Javaremi A. and Rahimi G. 2007. Detection of polymorphisms in two point of gene associated with Twinning (BMP-15) in Shal sheep. Pp. 483 in Proc. 5th Natio. Biotechnol. Cong. Iran. Tehran, Iran.
 

Iranian Journal of Applied Animal Science

Volume 4, Issue 4
December 2014
Pages 769-773

Files

History

  • Receive Date: 27 December 2013
  • Revise Date: 18 February 2014
  • Accept Date: 01 March 2014

Share

How to cite

Statistics