Association of Kappa-Casein Gene Polymorphism with some Biochemical Blood Indicators in Guilan Native Cattle of Iran (Bos indicus)

Document Type : Research Article


1 Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran

2 Department of Animal Science, Faculty of Agriculture Science, University of Agriculture and Natural Resource, Sari, Iran

3 Guilan Agriculture and Natural Resources Research Center, Rasht, Iran


Evaluation of the blood and milk biochemical parameters always is considered as effective key factor for animal health and dairy products. In order to assess the association of kappa-casein (K-CN) genetic variants with blood biochemical indicators, blood samples were randomly and individually collected from 126 Iranian Guilan native cattle. Blood plasma was used to measure blood glucose, urea, cholesterol, triglycerides and thyroxine concentrations. The genomic DNA was extracted from the whole blood by the modified Salting Out method. The 350 bp fragment of kappa-casein gene was amplified using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and a pair of specific primers was digested by HinfI restriction enzyme. In general, two alleles of A and B with the frequencies of 0.726 and 0.274 and two genotypes of AA and AB with frequencies of 0.452 and 0.548 were detected, respectively. The chi-square test result showed that the studied population was not in Hardy-Weinberg equilibrium. Results of statistical analysis revealed that there was significant difference between glucose, cholesterol and thyroxin levels in both sexes which are associated with the greater levels of testosterone and thyroid hormones in bulls than cows. However, there was no significant difference between males and females for urea and triglyceride levels (P>0.05). There was no significant difference between AA and AB genotypes of kappa-casein (K-CN) gene in Guilan native cattle for the blood parameters measured (P>0.05). According to the current results, kappa casein gene singly cannot be an appropriate marker to study the blood parameters in native cattle of Guilan.


Akers R.M. (2000). Selection for milk production from a lactation biology viewpoint. J. Dairy Sci. 83, 1151-1158.
Alipanah M. and Kalashnikova L.M. (2007). Influence of kapa-casein genetic variant on cheese making ability. J. Anim. Vet. Adv. 6(7), 855-857.
Alipanah M., Kalashnikova L. and Rodionov G. (2005). Kappa-casein genotypic frequencies in Russian breeds black and Red Pied cattle. Iranian J. Biotechnol. 3(3), 191-194.
Bittante B., Buttazzoni L., Spanghero M. and Aleandri R. (1987). Heritability of some blood variables and relationship with the performance traits of Italian Simmental young bulls. Pp. 146-149 in Proc.Euro. Assoc. Aviation Psychol. Semin., Wageningen, Netherland.
Bovenhius H., Arcndo V. and Korvea S. (1992). Association between milk protein polymorphism and milk production trait. J. Dairy Sci. 75, 2549-2559.
Buchberger J. and Dovc P. (2000). Lactoprotein genetic variants in cattle and cheese making ability. Food Technol. Biotechnol. 38, 91-98.
Galila A. and Darwish F. (2008). A PCR-RFLP assay to detect genetic variants of kappa-casein gene in cattle and buffalo. Arab J. Biotechnol. 11(1), 11-18.
Graml R., Olbrich-bludau A., Schwab M., Schallenberger E., Schams D. and Pirchner F. (1995). Relationship between plasma hormone and metabolite levels and breeding values of bulls. J. Anim. Breed. Genet. 112, 313-326.
Ijaz A., Lodhi L.A., Qureshi Z.I. and Yaunis M. (2004). Studies on blood golocuse, total proteine, urea and cholestrol levels in cyclic, non-cyclic and endometritic crossbred cows. Pakistan Vet. J. 24(2), 92-95.
Javanrouh A., Banabazi M.H., Esmaeilkhanian S., Amirinia C., Seyedabadi H.R. and Emrani H. (2006). Optimization on salting out method for DNA extraction from animal and poultry blood cells. Pp. 21-28 in Proc. 57th Ann. Meet. Europ. Asso. Anim. Product. Antalya, Turkey.
Klauzinska M., Zwierzchowski L., Siadkowska E., Szymanowska M., Grochowska R. and Urkowski M. (2000). Comparison of selected gene polymorphisms in polish red cattle and polish black-and-white cattle. Anim. Sci. Pap. Rep. 18(2), 107-116.
Lin C.Y., Sabour M.P. and Lee A.J. (1992). Direct typing to milk proteins as an aid for genetic improvement of dairy bulls and cows: a review. Anim. Breed. Abs. 60, 1-10.
Medrano J.F. and Aquilar-Cordova E. (1990). Genotyping of bovine kappa-casein loci following NA sequence amplification. Biotechnology. 8, 144-146.
Min S.H., Mccutcheon S.N., Mackenzie D.D.S. and Wickham B.W. (1993). Plasma metabolite and hormone concentrations in Friesian calves of low or high genetic merit effects of sex and age. Anim. Prod. 56, 17-27.
Nozad S., Ramin A.G. and Moghadam G. (2011). Diurnal variations in milk urea, protein and lactose concentration Holstein dairy cows. Acta Vet. Beograd. 61, 3-12.
Nozad S., Ramin A.G., Moghadam G., Asri-Rezaei S., Babapour A. and Ramin S. (2012). Relationship between blood urea, protein, creatinine, triglycerides and macro-mineral concentrations with the quality and quantity of milk in dairy Holstein cows. Vet. Res. Forum. 3(1), 55-59.
Oprzadek J., Tukaszewicz M., Dymnicki E. and Zwierzchowski L. (2003). Relationship between growth hormone, kappa-casein and ß-lactoglobulin genotype and selected biochemical blood indicators in young Friesian cattle. Anim. Sci. Pap. Rep. 21(4), 223-231.
Oprzaedek J., Dymnicki E., Zwierzchowski L. and Ukaszewicz M. (1999). The effect of growth hormone (GH), kappa-casein (CSN3) and ß-lactoglobulin (LGB) genotypes on carcass traits in Friesian bulls. Anim. Sci. Pap. Rep. 17, 85-92.
Qureshi M.S. (1998). Relationship of pre and postpartum nutritional status with reproductive performance in Nili-Ravi buffaloes under the conventional farming system in NWFP, Pakistan. Ph D. Thesis. Agriculture Univ., Faisalabad, Pakistan.
Robinson D.L., Hammond K., Graser H.U. and Mcdowell G.H. (1992). Relationships between breeding values and physiological responses to fasting and refeeding in dairy bulls. J. Anim. Breed. Genet. 109, 26-36.
SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC.
Schams D., Graf F., Meyer J., Graule B. and Mauthner M. (1991). Changes in hormones, metabolites and milk after treatment with sometribove (recombinant methionylbST) in Deutschesfleckvieh and German black and white cows. J. Anim. Sci. 69, 1583-1592.
Woolliams J.A. and Lovendahl P. (1991). Physiological attributes of male and juvenile cattle differing in genetic merit for milk yield: a review. Liv. Prod. Sci. 29, 1-16.
Zwierzchowski L., Elazowska B. and Grochowska R. (1995). Genotyping of kappa casein and growth hormone alleles in Polish black-and-white and Piedmonts cattle using PCR-RFLP technique. Anim. Sci. Pap. Rep. 13, 13-20.