The Effect of Curcumin on Acetaminophen-Induced Toxicity on Performance and some Blood Parameters of Japanese Quail from 0-37 Days of Age

Document Type : Research/Original Article


1 Department of Animal Science, Facultyof Agriculture,Payame Noor University, Tehran, Iran

2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran


This study was conducted to determine the effects of supplemented curcumin (CMN), derived from plant Curcuma longa, extract on performance and blood parameters of acetaminophen (ACT) induced quail hepatic injury. 240 quails from 0-37 days of age were used in a completely randomized design with 4 treatments of 4 replicates each and 15 chickens per replicate. All groups received 750 mg/kg body weight oral doses of ACT at 12 days of age. Japanese quails were divided into four groups: control (no CMN), commercial diet with 20 g CMN per ton, commercial diet with 40 g CMN per ton and commercial diet with 60 g CMN per ton. Feed intake and body weight gain were recorded weekly. Blood albumin, glucose, total protein, triglyceride, aspartate aminotransferase, alanine aminotransferase, bilirubin, uric acid and creatinine were determined at 28 and 35 days of age. Adding curcumin to the diets significantly increased serum aspartate aminotransferase at 28 days of age (P<0.05) and decreased triglyceride and bilirubin at 35 days of age (P<0.05). There were no significant differences in performance and other blood parameters among the treatment groups at 28 and 35 days of age. The data indicated that curcumin is a natural antioxidant hepatoprotective agent against hepatotoxicity induced by acetaminophen model. Thus, curcumin may have a therapeutic value in drug-induced hepatotoxicity as well as in acetaminophen therapy.


Ahmed H.H. and Mannaa F. (2004). Curcumin as an effective protective agent against etinylestradiol-induced hepatocellular cholestasis. Egyptian J. Med. Lab. Sci. 13, 1-15.
AL-Howiriny T.A., Adnan J., AL-Rehailyb A., Joanna R., Polsc J., Porter S. and Bahar A. (2005). Three new diterpenes and the biological activity of different extracts of Jasonia montana. Natur. Prod. Res. 19, 253-265.
Devi A.P. (1981). Studied on the toxicity of endosulfan to some fresh water fish with special reference to certain physiological changes induced in channa punctatus. Ph D. Thesis. Nagarjuna Univ., Nagarjuna, Nagar, South India.
Emadi M. and Kermanshahi H. (2007). Effect of turmeric rhizome powder on the activity of some blood enzymes in broiler chickens. Int. J. Poult. Sci. 6, 48-51.
Govindarajan V.S. (1980). Tumeric-chemistry, technology and quality. CRC critic review. Food Sci. Nutr. 12, 199-246.
Gowda N.K.S., Ledoux D.R, Rottinghaus G.E., Bermudez A.J. and Chen Y.C. (2008). Efficacy of Turmeric (Curcuma longa). Indian Materia Medica. Popular Book Depot. Bombay.
Hasheesh W.S., Maria M.A.S., Fakhary F.M. and Mohamed E.A.A. (2002). Influence of organophosphorus pesticide triazophos on some biochemical aspects in male albino rats. J. Egyptian German Soc. Zool. 37, 165-183.
Hussein M.A. (2008). Antidiabetic and antioxidant activity of Jasonia montana extract in Streptozotocininduced diabetic rats. Saudi Pharmaceut. J. 16, 214-221.
Hussein M.A. (2010). Protective effect of green tea against paraquat-induced hepatotoxicity in rats. Proc. 629-638 in 10th Sci. Vet. Med. Zagazic. Conf. Luxor.
Joe B., Vijaykumar M. and Lokesh B. (2004). Biological properties of curcumin-cellular and molecular mechanisms of action. Critic review. Food Sci. Nutr. 44, 97-111.
Kulkarni A.P. and Byczkowski J.Z. (1994). Hepatotoxicity. Pp. 459-490 in Introduction to Biochemical Toxicology. E. Hodgson and P. Levi, Eds. Appleton and Lange, Norwalk, CT.
Mahmoud H.M., Haggaga A.M. and El-Gebaly H.S. (2012). Toxicological studies of malathion on Japaness quail. Life Sci. J. 9, 1725-1732.
Malik G., Dahiya J.P. and Gera S. (2004). Biochemical studies on chlorpyrifos toxicity in broiler chickens. Indian J. Anim. Sci. 74, 473-476.
Masuda T., Isobe J., Jitoe A. and Nakatani N. (1992). Antioxidative curcuminoids from rhizomes of curcuma xanthorrhiza. Phytochemistry. 31, 3645-3647.
MasudaT., Hidaha K., Shinohara A., Mackawa T., Takeda Y. and Yamaguchi H. (1999). Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin. J. Agric. Food Chem. 47, 71-77.
Mathuria N. and Verma R. (2008). Ameliorative effect of curcumin on aflatoxin-induced toxicity in serum of mice. Acta Polon. Pharmaceut. Drug. Res. 65, 339-343.
Mc Lauchlan D.M. (1988). Creatinine, urate and urea. In Varleys Practical Clinical Biochemistry. A.D. Gowenlock, Ed. Heinemann medical books, London.
Murry M. (1994). Role of the liver in drug metabolism. Pp 3-21 in Drug-Induced Liver Disease. G.C. Farrell, Ed. Edinburg, Chuchill. Livigstone.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Okazaki Y., Iqbal M. and Okada S. (2005). Suppressive effects of dietary curcumin on the increased activity of renal ornithine decarboxylase in mice treated with a renal carcinogen, ferric nitrilotriacetate. Biochem. Biophys. Acta. Mol. Basis. Dis. 1740, 357-368.
Plaa G.L. and Hewitt W.R. (1982). Detection and evaluation of chemically induced liver injury. Pp. 407-440 in Principles and Methods of Toxicology. A.W. Hayes, Ed. Raven Press, New York.
Raymond B.B., Edward A.K. and Cohen S. (1991). Post-treatment protection with piperonyl butoxide against acetaminophen hepatotoxicity is associated with changes in selective but not total covalent binding. Adv. Exp. Med. Biol. 283, 689-692.
Reed D.J. (1994). Mechanism of chemically induced cell injury and cellular protection mechanisms. Pp. 265-295 in Introduction to Biochemical Toxicology. E. Hodgson and P. Levi, Eds. Wiley.
Sahu S.C. and Washington M.C. (1992). Effect of ascorbic acid and curcumin on quercetin-induced nuclear DNA damage, lipid peroxidation and protein degradation. Cancer Letter. 63, 237-241.
SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC., USA.
Slotkin T.A., Brown K.K. and Seidler F.J. (2005). Developmental exposure of rats to chloropyrifos elicitssex-selective hyperlipidemia and hyperinsulinemia in adulthood. Environ. Health Persepect. 113, 1291-1204.
Stacey N.H., Haschek W.M. and Winder C. (1993). Systemic toxicology. Pp. 37-76 in Occupational Toxicology. N.H. Statacey, Ed. Londom: Taylor and Francis Ltd.
Toda S., Miyase T., Arichi H., Tanizawa H. and Takino Y. (1985). Natural antioxidants III, antioxidant compounds isolated from rhizomes of curcuma longa. Chem. Pharmaceut. Bullet. 33, 1725-1728
Volume 5, Issue 1 - Serial Number 1
March 2015
Pages 203-207
  • Receive Date: 26 January 2014
  • Revise Date: 18 April 2014
  • Accept Date: 30 April 2014
  • First Publish Date: 01 March 2015