Effects of Coenzyme Q10 Supplementation on Growth Performance, some Hematological Parameters, Plasma Enzymes Activities in Broilers with Pulmonary Hypertension Syndrome (PHS)

Document Type: Research Article

Author

Department of Animal Science, Facultyof Agriculture, Payame Noor University, Tehran, Iran

Abstract

This study investigated the effects of coenzyme Q10 (CoQ10) supplementation on hematological, enzymes activities and biochemical parameters in broilers with pulmonary hypertension syndrome (PHS).Two hundred and forty 1-d-old Ross 308 male broiler chicks were randomly allocated into 3 groups with 4 replicates. From d 14, the diets were supplemented with CoQ10 at levels of 0, 20 and 40 mg/kg, respectively, while exposing them to low ambient temperature (10 to 15 ˚C) to induce PHSuntil d 42.Mortality was inspected to determine cause of death and diagnose of PHS. Hematological, biochemical and pathological tests were used to determine the incidence of PHS: total red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), plasma protein and glucose, activity of alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH).Blood samples were taken at d 21 and 42. At the end of the experiment (wk 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum and the ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated.Average BW gain and average feed intake were measured weekly from d 15 and average feed conversion ratio was calculated and reported weekly.The results showed that 40 mg/kg CoQ10 supplementationimprovedfeed conversion ratio(FCR)(P<0.05). Moreover, RBC count and plasma protein and glucose level were significantly decreased by 40 mg/kg CoQ10 compared to the other groups, but no significant changes were observed in HGB and HCT. The LDH activity decreased by CoQ10 supplementation (P<0.05). Measurement of malondialdehyde (MDA) content in plasma, RV / TV and mortality due to PHS decreased by 40 mg/kg CoQ10 supplementation (P<0.05). In conclusion, CoQ10 exerted preventive roles in PHS, possible through delivering antioxidant effect of cardiac myocytes and erythrocytes.

Keywords


Arab H.A., Jamshidi R., Rassouli A., Shams G. and Hassanzadeh M.H. (2006). Generation of hydroxyl radicals during ascites experimentally indued in broilers. Br. Poult. Sci. 47(2), 216-222.
Azuma J., Harada H., Sawamura A., Ohta H., Awata N., Yamauchi K., Kishimoto S. and Sperelakis N. (1985). Beneficial effect of coenzyme Q on myocardial slow action potentials in hearts subjected to decreased perfusion pressure-hypoxia- substrate-free perfusion. Basic Res. Cardiol. 80, 147-155.
Burton R.R., Besch E.L. and Smith A.H. (1968). Effect of chronic hypoxia on the pulmonary arterial blood pressure of the chicken. Am. J. Physiol. 214, 1438-1442.
Cawthon D., Beers K. and Bottje W.G. (2001). Electron transport chain defect and inefficient respiration may underlie pulmonary hypertension syndrome (ascites)-associated mitochondrialdysfunction in broilers. Poult. Sci. 80, 474-484.
Diaz-Cruz A., Nava C., Villanueva R., Serret M., Guinzberg R. and Pina E. (1996). Hepatic and cardiac oxidative stress and other metabolicchanges in broilers with the ascites syndrome. Poult. Sci. 75, 900-903.
Fathi M., Nazer Adl K., Ebrahimnezhad Y., Aghdam Shahryar H., daneshyar M. and Tanha T. (2011). The role of oxidative stress in development of congestive heart failure (CHF) in broilers with pulmonary hypertension syndrome (PHS). J. Anim. Vet. Adv. 10(20), 2724-2729.
Fathi M., Nazer Adl K., Ebrahimnezhad Y., Aghdam Shahryar H., Daneshyar M. and Tanha T. (2012). Plasma enzymes activities and antioxidant status characterization in pulmonary hypertension syndrome (PHS) in broilers. African J. Agric. Res. 7(5), 1414-1419.
Forsmark-Andre´e P., Lee C.P., Dallner G. and Ernster L. (1997). Lipid peroxidation and changes in the ubiquinone content and the respiratory chain enzymes of submitochondrial particles. Free Radic. Biol. Med. 22, 391-400.
Fujimoto S., Kurihara N., Hirata K. and Takeda T. (1993). Effects of CoQ10administration on pulmonaryfunction and exercise performance in patients with chroniclung diseases. Clin. Invest. 71, 162-166.
Geng A.L., Guo Y.M. and Yang Y. (2004). Reduction of ascites mortality in broilers by CoQ10. Poult. Sci. 83, 1587-1593.
Haramaki N., Stewart D.B., Aggarwal S., Ikeda H., Reznick A.Z. and Packer L. (1998). Networking antioxidants in the isolated rat heart are selectively depleted by ischemiareperfusion. Free Radic. Biol. Med. 25, 329-339.
Hemmin B. and Rajak C. (2006). CoQ10: absorption, tissue uptake, metabolism and pharmacokinetics. Free Radic. Res. 40(5), 445-453.
Ignarro L.J. (1989). Biological actions and properties of endothelium-derived nitricoxide formed and released from artery and vein. Circ. Res. 65, 1-21.
Julian R.J. (1993). Ascites in poultry. Avian Pathol. 22, 419-454.
Julian R.J., Friars G.W., French H. and Quinton M. (1987). The relationship of right ventricular hypertrophy, right ventricular failure, and ascites to weight gain in broiler and roaster chickens. Avian Dis. 31, 130-135.
Kale´n A., Norling B., Appelkvist E.L. and Dallner G. (1987). Ubiquinone biosynthesis by the microsomal fraction from rat liver. Biochim. Biophys. Acta. 926, 70-78.
Kato T. and Yoneda S. (1990). Reduction in blood viscosity by treatmentwith CoQ10in patients with ischemic heart disease. Int. J. Clin. Pharmacol. Ther. Toxicol. 28(3), 123-126.
Kumar A., Kaur H., Devi P. and Mohan V. (2007).Role of coenzyme Q10 (CoQ10) in cardiac disease, hypertension and Meniere-like syndrome. Pharmacol. Therapeut. 124, 259-268.
Li K., Qiao J., Zhao L., Dong S., Ou D., Wang J., Wang H. and Xu T. (2006). Increased calcium deposits and decreased Ca2 + -ATPase in right ventricular myocardium of ascitic broiler chickens. J. Vet. Med. 53, 458-463.
Littarru G.P. and Tiano L. (2007). Bioenergetic and antioxidant properties of CoQ10: recent developments. Mol. Biotechnol. 37, 31-37.
Maxwell M.H., Robertson G.W. and Moseley D. (1994). Potential role of serumtroponin T in cardiomyocyte injury in the broiler ascites syndrome. Br.Poult. Sci. 35, 663-667.
Maxwell M.H., Robertson G.W. and Mitchell M.A. (1993). Ultrastructuraldemonstration of mitochondrial calcium overload in myocardial cellsfrom broiler chickens with ascites and induced hypoxia. Res. Vet.Sci. 54, 267-277.
Mortensen S.A. (1993). Perspectives on therapy of cardiovascular diseases with CoQ10(ubiquinone). Clin. Invest. 71, 116-123.
Montaldo P.L., Fadda G., Salis S., Satta G., Tronci M., Cesare R.D., Reina R. and Concu A. (1991). Effects of the prolonged administration of CoQ10in borderline hypertensive patients: a hemodynamic study. Pp. 417-424 in Biomedical and Clinical Aspects of Coenzyme Q. K. Folkers, G.P. Littarru and T. Yamagami, Eds. Elsevier Science, Amsterdam.
Nakamura K., Noguchi K., Aoyama T., Nakajlma T. and Tanimura N. (1996). Protective effect of ubiquinone (CoQ9) on ascites in broiler chickens. Br. Poult. Sci. 37, 189-195.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Tankson J.D., Thaxton J.P. and Vizzier-Thaxton Y. (2002). Biochemicaland immunological changes in chickens experiencingpulmonary hypertension syndrome caused by Enterococcus faecalis.Poult. Sci. 81, 1826-1831.
Tribble D.L., Van Den Berg J.J., Motchnik P.A., Ames B.N., Lewis D.M., Chalt A. and Krauss R.M. (1994). Oxidative susceptibility of low density lipoprotein subfractions is related to their Q10 and alpha-tocopherol content. Proc. Natl. Acad. Sci. USA. 91, 1183-1187.
Upston J.M., Neuzil J., Witting P.K., Alleva R. and Stocker R. (1997). Oxidation of free fatty acids in low density lipoprotein by 15-lipoxygenase stimulates nonenzymic, α-tocopherol- mediated peroxidation of cholesterylesters. J. Biol. Chem. 272, 30067-30074.
Yokoyama H., Lingle D.M., Crestanello J.A., Kamelgard J., Kott B.R., Momeni R., Millili J., Mortensen S.A. and Whitman G.J. (1996). CoQ10protects coronary endothelial function from ischemia reperfusion injury via an antioxidant effect. Surgery. 120(2), 189-196.