Polymorphism in FSHβ and FSHRGenes and Their Relationship with Productive and Reproductive Performance in Iran Black, Arman and BaluchiSheep Breeds

Document Type: Research Article

Authors

Department of Animal Science, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran

Abstract

The objective of the present study was to detect polymorphism in follicle stimulating hormone receptor (FSHR) and beta subunit of follicle stimulating hormone (FSHβ) genes and their relation to litter size and body weight trait in Baluchi, Iran black and Arman sheep breeds. PCR-RFLP technique using ACCI and HinfI enzyme treatments employed to detection of polymorphism in FSHβ marker site but detected no polymorphism pattern in this locus. SSCP analysis used for more survey on detection of polymorphism in FSHβ marker site which resulted in AA and AC genotypes in Baluchi sheep, AA and AB genotypes in Iran black population and no polymorphism pattern detected in Arman breed and all samples showed AA genotype. Polymorphism in FSHR gene has been detected by treatment with MSCI restriction enzyme. Three genotypes of AA, AB and BB were observed among studied breeds. The B allele was found with the highest frequency in all tree breeds. Analysis of variance for the effects of theses marker loci was carried out using SAS program. Mean comparison by Duncan test showed that the effect of each FSHβ and FSHR marker sites on litter size has significant effect only in Baluchi sheep population. It seems mutant alleles can improve considerably mean of litter size than the wild type ones. Analysis of data for body weight and polymorphisms in different ages showed that there are no significant differences between genotypes of FSHβ locus and body weight trait in various ages in none of Baluchi, Iran-black and Arman breeds. Survey on polymorphisms of FSHR locus and body weight trait revealed significant difference in some ages only in Arman breed and wild type allele caused better performance. This information can be used to plan breeding programs for sheep breeds aimed at improving production and reproduction performance.

Keywords


Alinaghizadeh H., Mohammadabadi M.R. and Zakizadeh S. (2010). Exon 2 of BMP15 gene polymorphismin Jabal Barez red goat. J. Agric. Biotechnol. 2(1), 69-80.
An X.P., Han D., Hou J.X., Li G., Wang Y.N., Li L., Zhu G.Q., Wang J., Son Y.X. and Cao B.Y.(2010). Polymorphism of exon 2 of FSHβ gene and its relationship with reproduction performance in two goat breeds. Agricu. Sci. China. 9(6), 880-886.
Bassam B.J., Caetano-Anolles G. and Gresshoff P.M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196, 80-83.
Chappel S.C. and Howles C. (1991). Revaluation of the roles of luteinizing hormone and follicle-stimulating hormone in the ovulatory process. Hum. Reprod. 6, 1206-1212.
Chen K.F., Li N., Huang L.S., Zhang Q., Zhang J.S., Sun S.Q., Luo M. and Wu C.X. (2001). The combined genotypes effect ofESR and FSH beta genes on litter size traits in five different pig breeds. Chinese Sci. Bull. 46, 140-143.
Chu M.X., Jiao C.L., He Y.Q., Wang J.Y., Liu Z.H. and Chen G.H. (2007). Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Anim. Biotechnol. 18, 263-274.
De Castro F., Ruiz R., Montoro L., Hernandez D., Padilla E., Real L.M. and Ruiz A. (2003). Role of follicle-stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle stimulating hormone. Fertil. Steril. 80, 571-576.
Dickerson G.E. (1970). Efficiency of animal production molding the biological components. J. Anim. Sci. 30, 849-859.
Faure M.O., Nicol L., Fabre S., Fontaine J., Mohoric N., McNeilly A. and Taragnat C. (2005). BMP-4 inhibits follicle stimulating hormone secretion in ewe pituitary. J. Endocrinol. 186, 109-121.
Fogarty N.M. (2009). A review of the effects of the Booroola gene (FecB) on sheep production. Small Rumin. Res. 85, 75-84.
Gemzell C. (1975). Induction of ovulation in infertile women with pituitary tumors. Am. J. Obstet. Gynecol. 121, 311-315.
Hadizadeh M., Mohammadabadi M., Niazi A., Esmailizadeh A., Mehdizadeh Gazooei Y. and Molaei Moghbeli S. (2014). Use of bioinformatics tools to study exon 2 of GDF9 gene in Tali and Beetal goats. Mod. Genet. J. 8(3), 283-288.
Hanrahan J.P., Gergan S.M., Mulsant P., Mullen M., Davis G.H., Powell R.and Galloway S.M. (2004). Mutations in the genes for oocyte derived growth factors GDF 9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol. Reprod. 70, 900-909.
Hua G.H. (2006). Polymorphism analysis of five candidate genes related to reproduction traits and their effects on litter size in four goat breeds. MS Thesis. Huazhong Agric. Univ., Wuhan, Chinese.
Hunter M.G., Robinson R.S., Mann G.E. and Webb R. (2004). Endocrine and paracrine control of follicular development and ovulation rate in farm species. Anim. Reprod. Sci. 82, 461-477.
Lan Y.X., Chen H., Chen C.Y., Pan C.Y., Lie C.Z., Zhang Y.D. and Yu J. (2006). PCR-SSCP detection and DNA sequence analysis of exon 10 goat follicle-stimulating hormone receptor (FSHR) gene. J. Agric. Biotecnol. 14, 484-488.
Li J., Yang R.Q., Meng H. and Pan Y.C. (2004). Study on four candidate genes of litter size in Min pig. J. Shanghai Jiaotong Univ. Agric. Sci. 22(1), 74-77.
Li Y.J., Zhang L., Shang L.Q., Wang H.F., ZOU H., Zhang H. and Ji D.J. (2010). Genetic polymorphisms at three loci of PRLR and FSHR gene correlate with litter size in Chinese Haimen goat. J. Anim. Vet. Adv. 9(22), 2835-2838.
Liang C., Chu M.X., Zhang J.H., Liu W.Z., Fang L. and YE S.C. (2006). PCR-SSCP polymorphism of FSHß gene and its relationship with prolificacy of jining Grey goats. Hereditas. 28, 1071-1077.
Lotfi Farokhad M., Roshanfekr R., Amiri S., Mohamadi K. and Mirzadeh K.H. (2011). Genetic trends estimation for some of the growth traits in Arman sheep. J. Anim. Vet. Adv. 10(14), 1801-1803.
McGee E.A. and Hsueh A.J. (2000). Initial and cyclic recruitment of ovarian follicles. Endocrinol. Rev. 21, 200-214.
McNatty K.P., Smith P., Moore L.G., Reader K., Lun S., Hanrahan J.P., Groome N.P., Laitinen M., Ritvos O. and Juengel J.L. (2005). Oocyte-expressed genes affecting ovulation rate. Mol. Cell. Endocrinol. 234(1), 57-66.
Miller S.A., Dykes D.D. and Polesky H.F. (1988(. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids. Res. 16, 1215-1219.
Montgomery G.W., Tate M.L., Henry H.M., Penty J.M. and Rohan R.M. (1995). The follicle-stimulating hormone receptor and luteinizing hormone receptor genes are closely linked in sheep and deer. J. Mol. Endocrinol. 15, 259-265.
Rashidi A. (2012). Genetic parameter estimates of body weight traits in Iran-Black sheep. J. Livest. Sci. Technol. 1(1), 54-60.
SAS Institute. (2006). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Segaloff D.L. and Ascoli M. (1993). The lutropin / choriogonadotropin (LH.CG) receptor 4 years later. Endocrinol. Rev. 14, 324-347.
Simoni M. and Nieschlag E. (1995). FSH in therapy: physiological basis, new preparations and clinical use. Reprod. Med. Rev. 4, 163-177.
Simoni M., Gromoll J. and Nieschlag E. (1997). The follicle-stimulating hormone receptor biochemistry, molecular biology, physiology and pathophysiology. Endocrinol. Rev. 18, 739-773.
Tahmoorespur M. and Sheikhloo M. (2011). Pedigree analysis of the closed nucleus of Iranian Baluchi sheep. Small Rumin. Res. 99(1), 1-6.
Wang X., Wang A., Fu J. and Lin H. (2006). Effects of ESR1, FSHB and RBP4 genes on litter size in a Large White and a Landrace Herd. Arch. Tierz. Dummerstorf. 49(1), 64-70.
Xu N.Y., Zhang S.Q. and Peng S.H. (2003). Investigation on the distribution and their effects on reproduction traits of three major genes in Jinhua pigs. Acta Genet. Sin. 30, 1090-1096.
Yang W.C., Li S.J., Tang K.Q., Hua G.H., Zhang C.Y., YU J.N., Han L. and Yang L.G. (2010). Polymorphisms in the 5´ upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows. Anim. Reprod. Sci. 119, 172-177.
Yaofeng Z., Ning L.I., Lu X., CaoGengsheng C., Yizhen C., Shun Z., Yongfu C., Changxin W., Jiansheng Z., Shiquan S. and Xueqing X. (1998). FSHΒ subunit gene is associated with major gene controlling litter size in commercial pig breeds. Sci. China. 41, 665-668
Yun T., Bin J.I. and Yun C. (2007). Effect of the 5'-flanking region of goat follicole-stimulating hormone receptor gene on yean trail. J. Northwest A F Univ. 35, 9-14.
Zhang C.Y., Wu C.J., Zeng W.B., Huang K.K., Li X., Feng J.H., Wang D., Xu D.Q., Wen Q.Y., Yang L.G. and Hua G.H. (2011). Polymorphism in exon 3 of follicle stimulating hormone beta (FSHB) subunit gene and its association with litter traits and superovulation in the goat. Small Rum. Res. 96, 53-57.
Zhao L.H., Chen J.L., Xu H., Liu J.W. and Xu R.F. (2010). Cloning and expression of FSHb gene and the effect of FSH on the mRNA level of FSHR in the local chicken. Asian-australas J. Anim. Sci. 23(3), 287-416.