Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
Receive Date: 26 April 2014,
Revise Date: 03 June 2014,
Accept Date: 15 June 2014
This study was conducted to compare digestibility of wheat straw (WS) by fungi and whole rumen microorganisms (WRM). Dry matter (DM), neutral and acid detergent fiber (NDF and ADF) digestibility of WS were compared with in vitro digestion (IVD), gas production (GP) and specific rumen anaerobic fungi culture (SRAFC). Dry matter, NDF and ADF digestibility of WS by WRM of buffalo (60.80, 49.93 and 17.45%, respectively) were more than cattle (53.00, 38.63 and 10.62%, respectively) (P<0.05). Regardless the type of microorganisms, digestibility of DM (P>0.05), NDF and ADF by buffalo (51.03, 44.41 and 12.09%) was more than cattle (48.04, 36.34 and 8.76) (P<0.05). Potential of GP (B) by fungi and WRM in cattle was more than buffalo (P<0.05). Rate of GP (C) for WRM and fungi of cattle was less than buffalo (P<0.05). Regardless the type of microorganisms, C in buffalo was more than cattle (P<0.05) and was vice versa for B (P<0.05). Regardless the type of animal species, WRM had higher digestibility and B than fungi (P>0.05), but rate of GP of them was same. In SRAFC, DM digestibility of WS by fungi of buffalo at days 3 and 12 was more than cattle (P<0.05). The number of fungi in cattle rumen was more than buffalo (P<0.05). The potential of fungi and WRM of buffalo were more than cattle. Therefore, the results were shown the advantage and supremacy of buffalo in usage the low quality roughages.
Akin D.E. and Borneman W.S. (1990). Role of rumen fungi in fiber degradation. J. Diary Sci. 73, 3023-3032. Agarwal N., Kewalramani N., Kamra D.N., Agarwal D.K. and Nath K. (1991). Hydrolytic enzymes of buffalo rumen comparison of cell free rumen fluid, bacterial and protozoal fractions. Buffalo J. 7, 203-207. Bahatia S.K., Kumar S. and Sangwan D.C. (2004). Advances in Buffalo-Cattle Nutrition and Rumen Ecosystem. International Book Distributing Co. Bauchop T. and Clarke T.J. (1976). Attachment of the ciliate epidinium crawley to plant fragments in the sheep rumen. Appl. Environ. Microbiol. 32, 417-422. Broderick G.A. and Kang J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Diary Sci. 63, 64-75. Bryant M.P. and Small N. (1960). Observations on the ruminal microorganisms of isolated and inoculated calves. J. Diary Sci.43, 654-67. BryantM.P. (1973). Nutritional requirements of the predominant rumen cellulolytic bacteria. Federation Proc.32(7), 1809-1813. Chaji M. and Mohammadabadi T. (2011). The investigation of in vitro fermentation of sugarcane pith treated with low temperature steam and sulfuric acid by isolated rumen microbial fractions. Anim. Nutr. Feed Technol. 11, 185-193. Chaudhary L.C., Srivastava A. and Singh K.K. (1995). Rumen fermentation pattern and digestion of structural carbohydrates in buffalo (Bubalus bubalis) calves as affected by ciliate protozoa. Anim. Feed Sci. Technol. 56, 111-117. Chen X.L. and Wang J.K. (2008). Effects of chemical treatments of rice straw on rumen fermentation characteristics, fibrolytic enzyme activities and populations of liquid and solid associated ruminal microbes in vitro. Anim. Feed Sci. Technol. 141, 1-14. Davies D.R., Theodorou M.K., Lawrence M.I. and Trinci A.P.J. (1993). Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces. J. Gen. Microbiol. 139, 59-64. Dayanand T.L., Nagpal R., Puniya A.K., Sehgal J.P. and Singh K. (2007). Biodegradation of urea-NH3treated wheat straw using anaerobic rumen fungi. J. Anim. Feed Sci. 16, 484-489. Dehority B.A. (2003). Rumen Microbiology. London, UK. Nottingham University, Academic Press. UK. Dey A., Sehgal J.P., Puniya A.K. and Singh K. (2004). Influence of anaerobic fungal culture (Orpinomycessp) administration on growth rate, ruminal fermentation and nutrient digestion in calves. Asian-australs J. Anim. Sci. 17, 820-824. Erfle J.D., Boila R.J., Teather R.M., Mahadevan S. and Sauer F.D. (1982). Effect of pH fermentation characteristics and protein degradation by rumen micro-organisms in vitro. J. Diary Sci. 65, 1451-1464. Hobson P.N. and Stewart C.S. (1997). The Rumen Microbial Ecosystem. London: Chapman and Hall. Hungate R.E. (1966). The Rumen and Its Microbes. London: Academic Press. UK. Jabbari S. (2010). The comparison digestibility of steam treated sugarcane pith and wheat straw by rumen microorganisms of cattle and buffalo in Khuzestan. MS Thesis. Khuzestan Ramin Agricultural and Natural Resources Univ., Ahvaz, Iran. Jabbari S., Eslami M., Chaji M., Mohammadabadi T. and Bojarpour M. (2011). The Comparison of in vitro digestibility of wheat straw by rumen microorganism of khuzestani buffalo and hostein cow in vitro digestibility by Khuzestani buffalo. Pp. 266-268 in Proc. Int. Conf. Biol., Environ. Chem.Hongkong, China. Jabbari S., Eslami M., Chaji M., Mohammadabadi T. and Bojarpour M. (2012). Acomparison between water buffalo (Khuzestani) and cow rumen fluids in terms ofthe in vitro digestibility of steam treated sugarcane pith. Pp. 405-406 in Proc. WCDS Adv. Dairy Technol., Univ. Alberta, Canada. Krause D.O., Denman S.E., Mackie R.I., Morrison M., Rae A.L., Attwood G.T. and McSweency C.S. (2003). Opportunities to improve fiber degradation in the rumen: microbiology, ecology and genomics. FEMS Microbiol. Rev. 27, 663-693. Kumar S., Singh S. and Bhatia S.K. (2002). Microbial and biochemical changes in the rumen of cattle and buffalo fed oat hay concentrate diet. Indian J. Anim. Nutr. 19,78-79. Lee S.S., Choi C.K., Ahn B.H., Moon Y.H., Kim C.H. and Ha J.K. (2004). In vitro stimulation of rumen microbial fermentation by a rumen anaerobic fungal culture. Anim. Feed Sci. Technol. 115, 215-226. Lowe S.E. (1987). Cellulases and xylanase of an anaerobic rumen fungusgrown on wheat straw, wheat straw holocellulose, cellulose and xylan. Appl. Environ. Microbiol. 53, 216-1223. Malakar D. and Walli T.K. (1995). Relative fibre degradation in vitro by bacteria and fungi using inoculum from cow and buffalo rumen. Indian J. Diary Sci. 48, 295-301 Mansouri H., Nik-Khah A., Rezaeian M. and Mirhadi S.A. (2005). Comparison of microbial population in ruminal fluid of Sistani and Holstein cattle fed different roughages. Pajouhesh and Sazandegi. 72, 66-73. McDougall E.L. (1948). Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochem. J. 43, 99-106. Menk K.H. and Stenigass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 6-55. NRC. (1996). Nutrient Requirements for Beef Cattle. 7th Ed. National Academy Press, Washington, DC, USA. Osorio H. and Cruz D.L. (1990). Steam treated bagasse for fattening cattle. Effect of supplementation with Giricidia sepium and urea / molasses. Lives. Res. Rural Dev. 2(2), 77-91. Paul S.S., Kamra D.N., Sastry V.R.B. and Agarwal N. (2004). Effect of administration of an anaerobic gut fungus isolated from wild blue bull to buffaloes on in vivo ruminal fermentation and digestion of nutrients. Anim. Feed Sci. Technol. 115, 143-157. Rafiei M., Chaji M., Mohammadabadi T. andSari S. (2013). The comparison digestibility of steamtreated sugarcane pith by rumen bacteria or rumen microorganisms of Holstein cow and buffalo of Khuzestan. J. Rumin. Res. 1(1), 53-75. Rezaeian M., Beakes G.W. and Chaudhry A.S. (2005). Relative fibrolytic activities of anaerobic rumen fungi on untreated and sodium hydroxide treated barley straw in in vitro culture. Anaerobe.11, 163-175. Russell J.B. (1986). Ecology of rumen microorganism: energy use. Pp. 256 in Aspect of Digestive Physiology in Ruminants. A. Dabson and M.J. Dobson, Eds. London, Comstock Publishing Association, UK. Samanta A.K., Walli T.K., Batish V.K., Grover S., Rajput Y.S. and Mohanty A.K. (1999). Characterization of anaerobic fungi in rumen of riverine buffalo. Indian J. Anim. Nutr. 16(4), 275-278. SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC. USA. Sehgal J.P., Jit D., Puniya A.K. and Singh S. (2008). Influence of anaerobic fungal administration on growth, rumen fermentation and nutrient digestion in female buffalo calves. J. Anim. Sci. 17, 510-518. Singh S., Bhatia S.K. and Pradhan K. (2003). Relative ruminal ciliates distribution and physiology of bacteria isolated in buffalo and cattle fed wheat straw-preformed protein diets. Indian J. Anim. Sci. 73, 663-664. Singh S., Pradhan K., Bhatia S.K., Sangwan D.C. and Sagar V. (1992). Relative rumen microbial profile of cattle and buffalo fed wheat straw-concentrate diets. Indian J. Anim. Sci. 62, 1197-1198. Tewatia B.S. and Bhatia S.K. (1998). Comparative studies in rumen ammonia anabolizing enzymes, microbial and mineral profiles between buffalo and cattle fed fibrous diet. Buffalo J. 12, 169-170. Tilley J.M. and Terry R.A. (1963). A two staged technique for the in vitro digestion of forage crops. J. Br. Grassl. Soc. 10, 104-111. Theodorou M.K., Gill M., King-Spooner C. and Beever D.E. (1990). Enumeration of anaerobic chytridiomycetes as thallus-forming units: novel method for quantification of fibrolytic fungal populations from the digestive tract ecosystem. Appl. Environ. Microbiol.56(4), 1073-1078. Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Methods of dietary fiber, neutral detergentfiber and nonstarch polysaccharides in relation to animal nutrition. J. Diary Sci. 74, 3583-3597. Wallace R.J. and Joblin K.N. (1985). Proteolytic activity of a rumen anaerobic fungus. FEMS Microbiol. Lett. 29, 19-26. Wanapat M. (2001). Swamp buffalo rumen ecology and its manipulation. Pp. 155-187 in 1th Natio. Workshop on Swamp Buffalo Develop. Thailand. Wanapat M. and Pimpa O. (1999). Effect of ruminal NH3-N levels on ruminal fermentation purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian-australs J. Anim. Sci. 12, 904-907. Wanapat M., Pilagun R. and Kongmun P. (2009). Rominal ecology of swamp buffalo as influenced by dietary source. Anim. Feed Sci. Technol. 151, 205-214.